Search results for "BREAST"

showing 10 items of 1871 documents

Limitations of Immunohistochemistry in Diagnosis of a Primary Mucinous Carcinoma of the Skin and Its Metastasis

2020

We describe the case of a 67-year-old female patient who presented with an unclear swelling on the right supraclavicular neck, two years after resection of a mucinous carcinoma on the right mastoid. Two pathological reports from separate universities diagnosed the primary mastoid skin lesion as a metastatic adenocarcinoma. Strikingly, GATA binding protein 3 (GATA3) and mammaglobin, both immunomarkers found in breast cancer, were positive. An urgent search for the primary tumor most likely in the breast was commenced. However, as no other primary tumor could be detected at that time, a Primary Mucinous Carcinoma of the Skin (PMCS) was also debated. Two years later neck node enlargement was s…

Pathologymedicine.medical_specialtyContext (language use)ImmunostainingMetastasisLesion030207 dermatology & venereal diseases03 medical and health sciences0302 clinical medicineMammaglobinBreast cancerDiagnosisCarcinomaMedicineMucinous carcinomabiologybusiness.industryCarcinomaAsploro Journal of Biomedical and Clinical Case ReportsGeneral Medicinemedicine.diseaseImmunohistochemistryPrimary tumor030220 oncology & carcinogenesisbiology.proteinmedicine.symptombusinessAsploro Journal of Biomedical and Clinical Case Reports
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Computer-aided diagnosis in digital mammography: comparison of two commercial systems

2014

Aim: Within this work, a comparative analysis of two commercial computer-aided detection or diagnosis (CAD) systems, CyclopusCAD® mammo (v. 6.0) produced by CyclopusCAD Ltd (Palermo, Italy) and SecondLook® (v. 6.1C) produced by iCAD Inc. (OH, USA) is performed by evaluating the results of both systems application on an unique set of mammographic digital images routinely acquired in a hospital structure. Materials & methods: The two CAD systems have been separately applied on a sample set of 126 mammographic digital cases, having been independently diagnosed by two senior radiologists. According to the human diagnosis, the cases in the sample reference set are divided into 61 negatives and 6…

Pathologymedicine.medical_specialtyDigital mammographyRadiological and Ultrasound Technologymedicine.diagnostic_testbusiness.industryCad systemSettore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)Digital imageComputer-aided diagnosisBreast cancer clusters computer-assisted diagnosis FFDM FROC curve mammografy mass lesions microcalcifications performancemedicineMammographyRadiology Nuclear Medicine and imagingRadiologybusiness
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Unmasking epithelial-mesenchymal transition in a breast cancer primary culture: a study report

2012

Abstract Background Immortalized cancer cell lines are now well-established procedures in biomedicine for a more complete understanding of cellular processes in cancer. However, they are more useful in preparation of fresh tumour tissue, in order to obtain cancer cells with highly preserved individual tumour properties. In the present study we report an analytical investigation on a breast cancer primary cell culture isolated from a surgical specimen obtained from a patient with an infiltrating ductal carcinoma. The objective of the research was to reveal unrecognized aspects of neoplastic cells, typical of the tumour from where the cells were derived, but masked in fixed tissue sections, i…

Pathologymedicine.medical_specialtyEpithelial-Mesenchymal TransitionImmunocytochemistryShort Reportlcsh:MedicineBreast NeoplasmsBiologyGeneral Biochemistry Genetics and Molecular BiologyCell LineBreast cancerBreast cancerCarcinomamedicineBiomarkers TumorTumor Cells CulturedHumansVimentinMicroscopy Phase-ContrastEpithelial–mesenchymal transitionPrimary cell culturelcsh:Science (General)lcsh:QH301-705.5Medicine(all)Biochemistry Genetics and Molecular Biology(all)Reverse Transcriptase Polymerase Chain Reactionlcsh:RMesenchymal stem cellCarcinoma Ductal BreastCancerMuscle SmoothBreast cancer Primary cell culture Epithelial-mesenchymal transition (EMT)General Medicinemedicine.diseaseCadherinsImmunohistochemistryActinsGene Expression Regulation NeoplasticEpithelial-mesenchymal transition (EMT)lcsh:Biology (General)Cell cultureCancer cellKeratinsFemalelcsh:Q1-390
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Skin involvement as the first manifestation of breast implant-associated anaplastic large cell lymphoma

2016

Breast implant-associated anaplastic large cell lymphoma (ALCL) is a newly described clinical and pathologic entity that typically presents as seroma in the fibrous scar around the implant. Less frequently, it presents as a solid peri-implant mass, and there have been no reports to date of cutaneous lesions as the presenting manifestation. We report the case of a 56-year-old woman with a history of bilateral breast reconstruction following breast cancer of the right breast who consulted with several papules on the right breast suggestive of metastasis. Histopathology showed a proliferation of large epithelioid lymphocytes with highly pleomorphic cells and nuclei. The neoplastic cells were C…

Pathologymedicine.medical_specialtyHistologyCD30business.industryDermatologyCD15Gene rearrangement030230 surgerymedicine.diseasePathology and Forensic MedicineMetastasislaw.invention03 medical and health sciences0302 clinical medicineBreast cancerlawhemic and lymphatic diseases030220 oncology & carcinogenesisBreast implantmedicineAdenocarcinomabusinessAnaplastic large-cell lymphomaJournal of Cutaneous Pathology
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Nodular morphea keloidal type: A rare case with paradigmatic histopathology significantly accompanied by a flawless surgical scar

2020

Nodular morphea is a rare variant of localized scleroderma, clinically and histopathologically characterized by cutaneous nodules or plaques associated or superimposed to the flat lesions of classic morphea. Accordingly, the association of such outgrowths with systemic sclerosis is designated as nodular scleroderma. Sometimes these lesions appear as firm, erythematous and irregularly curvy plaques resembling keloids or hypertrophic scars, thus characterizing keloidal morphea or keloidal scleroderma. These mystifying features can make the diagnosis challenging, especially in the absence of a well‐documented medical history. Here we report a case of keloidal morphea with multiple histopatholo…

Pathologymedicine.medical_specialtyHistologybusiness.industryBreast NeoplasmsDermatologyMiddle Agedunusual immunohistochemical featuremedicine.diseasePathology and Forensic MedicineScleroderma LocalizedKeloidRare casemedicineHumansFemaleHistopathologySurgical scarbusinessbland surgical scarMorphea
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CD1a down-regulation in primary invasive ductal breast carcinoma may predict regional lymph node invasion and patient outcome

2007

Aims: CD1a is a molecule belonging to the highlyconserved group of CD1 proteins. Its expression indendritic cells is related to the presentation of tumour-derived glycolipid antigens to T cells and, consequently,the development of a successful antitumour response.The aim was to investigate the presence of CD1a+ cellsin both primary tumours and lymph nodes (LN) ofa series of 35 invasive ductal carcinomas by bothimmunohistochemistry and reverse transcription-poly-merase chain reaction.Methods and results: CD1a antigen was more expressedin N0 than N1 breast cancer (P < 0.0001) in bothprimary lesions and LN metastases and correlatedpositively and significantly with oestrogen (ER) (P =0.0025) and…

Pathologymedicine.medical_specialtyHistologybusiness.industryCancerGeneral MedicineDendritic cellDuctal carcinomamedicine.diseasePathology and Forensic Medicinemedicine.anatomical_structureBreast cancerProgesterone receptormedicineCarcinomaskin and connective tissue diseasesAntigen-presenting cellbusinessLymph nodeHistopathology
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Cytokeratin 8 immunostaining pattern and E-cadherin expression distinguish lobular from ductal breast carcinoma.

2000

Immunohistochemistry using antibodies to cytokeratin 8 can serve as a valuable diagnostic tool for the differentiation of lobular from ductal carcinomas of the breast. In contrast with ductal carcinomas, which exhibit a peripheral-predominant immunostaining pattern, adjacent tumor cells “molding” to each other, lobular carcinomas exhibit a ring-like perinuclear immunostaining pattern, creating a “bag of marbles” appearance with neighboring tumor cells. This immunostaining pattern is stable even in the tumors that otherwise do not exhibit characteristic histomorphologic features (ie, solid or pleomorphic type of a lobular carcinoma) and tumors that mimic growth patterns characteristic of the…

Pathologymedicine.medical_specialtyLobular carcinomaIntermediate FilamentsBreast NeoplasmsBiologyDiagnosis DifferentialImmunoenzyme TechniquesCytokeratinBreast cancerCarcinomamedicineHumansskin and connective tissue diseasesCell NucleusCarcinoma Ductal BreastGeneral MedicineDuctal carcinomamedicine.diseaseCadherinsDuctal Breast CarcinomaCarcinoma LobularInvasive lobular carcinomaKeratin 8KeratinsFemaleAmerican journal of clinical pathology
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Automated Measurement of Microcirculatory Blood Flow Velocity in Pulmonary Metastases of Rats

2014

Because the lung is a major target organ of metastatic disease, animal models to study the physiology of pulmonary metastases are of great importance. However, very few methods exist to date to investigate lung metastases in a dynamic fashion at the microcirculatory level, due to the difficulty to access the lung with a microscope. Here, an intravital microscopy method is presented to functionally image and quantify the microcirculation of superficial pulmonary metastases in rats, using a closed-chest pulmonary window and automated analysis of blood flow velocity and direction. The utility of this method is demonstrated to measure increases in blood flow velocity in response to pharmacologi…

Pathologymedicine.medical_specialtyLung NeoplasmsIntravital MicroscopyGeneral Chemical EngineeringBreast NeoplasmsGeneral Biochemistry Genetics and Molecular BiologyMicrocirculationNeovascularizationRats NudeCell Line TumormedicineAnimalsHumansCancer BiologyLungNeovascularization PathologicGeneral Immunology and Microbiologybusiness.industryMicrocirculationGeneral NeuroscienceBlood flowmedicine.diseaseRatsmedicine.anatomical_structureHeterograftsFemaleSarcoma ExperimentalSarcomamedicine.symptombusinessPulmonary tumorBlood Flow VelocityTarget organIntravital microscopyJournal of Visualized Experiments
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Antiamphiphysin-positive stiff-person syndrome associated with small cell lung cancer

2006

The paraneoplastic amphiphysin(+) stiff-person syndrome (SPS) has so far only been described in women with breast adenocarcinoma. Here, we describe the rare case of a female patient with antiamphiphysin(+) SPS due to small cell cancer of the lung.

Pathologymedicine.medical_specialtyLung NeoplasmseducationNerve Tissue ProteinsStiff-Person SyndromeBreast AdenocarcinomaSmall-cell carcinomafluids and secretionsmedicineCarcinomaHumansCarcinoma Small CellLung cancerAutoantibodiesLungElectromyographybusiness.industryfungiRespiratory diseaseMiddle Agedequipment and suppliesmedicine.diseasemedicine.anatomical_structureNeurologyAmphiphysinFemaleNeurology (clinical)businessStiff person syndromeMovement Disorders
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Identifying the primary site of origin of MRI brain metastases from lung and breast cancer following a 2D radiomics approach

2017

Detection of brain metastases in patients with undiagnosed primary cancer is unusual but still an existing phenomenon. In these cases, identifying the cancer site of origin is non-feasible by visual examination of magnetic resonance (MR) images. Recently, radiomics has been proposed to analyze differences among classes of visually imperceptible imaging characteristics. In this study we analyzed 46 T1-weighted MR images of brain metastases from 29 patients: 29 of lung and 17 of breast origin. A total of 43 radiomics texture features were extracted from the metastatic lesions. Support vector machine (SVM) and k-nearest neighbors (k-NN) classifiers were implemented to evaluate the classificati…

Pathologymedicine.medical_specialtyLungmedicine.diagnostic_testbusiness.industryFeature extractionCancerMagnetic resonance imagingmedicine.disease030218 nuclear medicine & medical imagingSupport vector machine03 medical and health sciences0302 clinical medicineBreast cancermedicine.anatomical_structureRadiomicsmedicineRadiologybusinessQuantization (image processing)030217 neurology & neurosurgery2017 IEEE 14th International Symposium on Biomedical Imaging (ISBI 2017)
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