Search results for "BST"

showing 10 items of 10311 documents

Cyclophosphamide plus vincristine and prednisone in the treatment of severe pemphigus vulgaris refractory to conventional therapy

1992

Five patients with severe pemphigus vulgaris refractory to conventional therapy with azathioprine and corticosteroids were treated with cyclophosphamide, vincristine and prednisone. One patient was not evaluable, while the remaining four patients showed a complete response. Duration of response was in the range of 13-94 months. Toxicity was mainly represented by alopecia, myelosuppression and gastrointestinal side-effects such as nausea/vomiting. Although cyclophosphamide and vincristine may induce severe side-effects, this association may be useful in controlling severe disease resistant to previous conventional therapies.

0301 basic medicineMalemedicine.medical_specialtyVincristineCyclophosphamideAdolescentNausea030106 microbiologyAzathioprinemacromolecular substancesGastroenterology03 medical and health sciences0302 clinical medicineRefractoryPrednisoneInternal medicinemedicineHumansPharmacology (medical)CyclophosphamideAgedPharmacologybusiness.industryPemphigus vulgarisMiddle Agedmedicine.diseaseInfectious DiseasesOncologyVincristine030220 oncology & carcinogenesisVomitingPrednisoneDrug Therapy CombinationFemalemedicine.symptombusinessPemphigusmedicine.drug
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Decrease in αβ/γδ T-cell ratio is accompanied by a reduction in high-fat diet-induced weight gain, insulin resistance, and inflammation.

2018

The implication of αβ and γδ T cells in obesity-associated inflammation and insulin resistance (IR) remains uncertain. Mice lacking γδ T cells show either no difference or a decrease in high-fat diet (HFD)-induced IR, whereas partial depletion in γδ T cells does not protect from HFD-induced IR. αβ T-cell deficiency leads to a decrease in white adipose tissue (WAT) inflammation and IR without weight change, but partial depletion of these cells has not been studied. We previously described a mouse model overexpressing peroxisome proliferator-activated receptor β (PPAR-β) specifically in T cells [transgenic (Tg) T-PPAR-β] that exhibits a partial depletion in αβ T cells and no change in γδ T-ce…

0301 basic medicineMalemedicine.medical_specialtymedicine.medical_treatmentT cellReceptors Antigen T-Cell alpha-betaT-LymphocytesAdipose tissueInflammationWhite adipose tissueDiet High-FatWeight GainBiochemistry03 medical and health sciencesMice0302 clinical medicineImmune systemInsulin resistanceInternal medicineGlucose IntoleranceGeneticsmedicineAnimalsObesityMolecular BiologyInflammationChemistryInsulinWeight changeBody Weightfood and beveragesnutritional and metabolic diseasesReceptors Antigen T-Cell gamma-deltamedicine.diseaseMice Inbred C57BL030104 developmental biologyEndocrinologymedicine.anatomical_structurelipids (amino acids peptides and proteins)medicine.symptomInsulin Resistancehormones hormone substitutes and hormone antagonists030217 neurology & neurosurgeryBiotechnologyFASEB journal : official publication of the Federation of American Societies for Experimental Biology
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Influence of endogenous glucagon-like peptide-2 on lipid disorders in mice fed a high-fat diet

2016

Aim: The purpose of the present study was to investigate the influence of endogenous glucagon-like peptide-2 (GLP-2) on lipid profile in mice fed a standard diet (STD) or a high-fat diet (HFD). Materials and methods: HFD- and age-matched STD mice were injected once a day with GLP-2 (3-33), a GLP-2 receptor (GLP-2R) antagonist, or vehicle for 4 weeks. Results: HFD mice displayed increased intrahepatic lipid concentration and hepatic steatosis and higher plasma concentrations of cholesterol, LDL, AST, and ALT than STD mice. No difference was observed in lipid fecal elimination. In STD mice, the chronic treatment with GLP-2 (3-33) did not affect any parameter, while in HFD mice, it enhanced pl…

0301 basic medicineMalemedicine.medical_specialtyobesityEndogenyBiologyDiet High-FatliverSettore BIO/09 - Fisiologia03 medical and health scienceschemistry.chemical_compoundMice0302 clinical medicineInsulin resistanceEndocrinologyMetabolic DiseaseslipidInternal medicineinsulin resistancemedicineGlucagon-Like Peptide 2AnimalsReceptormedicine.diagnostic_testCholesterolSettore BIO/16 - Anatomia Umanadigestive oral and skin physiologyAntagonistGeneral Medicinemedicine.diseaseGlucagon-like peptide-2LipidsPeptide FragmentsMice Inbred C57BL030104 developmental biologyEndocrinologychemistrylipids (amino acids peptides and proteins)030211 gastroenterology & hepatologySteatosisLipid profileGLP-2hormones hormone substitutes and hormone antagonists
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IL-17A-associated IKK-α signaling induced TSLP production in epithelial cells of COPD patients.

2018

Thymic stromal lymphopoietin (TSLP) is a cytokine expressed in the epithelium, involved in the pathogenesis of chronic disease. IL-17A regulates airway inflammation, oxidative stress, and reduction of steroid sensitivity in chronic obstructive pulmonary disease (COPD). TSLP and IL-17A were measured in induced sputum supernatants (ISs) from healthy controls (HC), healthy smokers (HS), and COPD patients by enzyme-linked immunosorbent assay. Human bronchial epithelial cell line (16HBE) and normal bronchial epithelial cells were stimulated with rhIL-17A or ISs from COPD patients to evaluate TSLP protein and mRNA expression. The effects of the depletion of IL-17A in ISs, an anticholinergic drug,…

0301 basic medicineMalemedicine.medical_treatmentClinical Biochemistrylcsh:MedicineCell CountBiochemistryCholinergic AntagonistsPathogenesisHistonesPulmonary Disease Chronic Obstructivelcsh:QD415-436COPDKinaseInterleukin-17AcetylationI-kappa B KinaseRespiratory Function TestsCytokinemedicine.anatomical_structureMolecular MedicineCytokinesFemaleProtein BindingSignal TransductionThymic stromal lymphopoietinEnzyme-Linked Immunosorbent AssayRespiratory MucosaArticleCell Linelcsh:Biochemistry03 medical and health sciencesThymic Stromal LymphopoietinmedicineGene silencingCOPDHumansMolecular Biologybusiness.industrylcsh:RSputumEpithelial Cellsmedicine.diseaseEpitheliumrespiratory tract diseases030104 developmental biologyCell cultureCancer researchbusinessBiomarkersExperimentalmolecular medicine
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Acute and chronic nicotine exposures differentially affect central serotonin 2A receptor function : focus on the lateral habenula

2020

Nicotine addiction is a serious public health problem causing millions of deaths worldwide. Serotonin (5-hydroxytryptamine

0301 basic medicineMalenucleus accumbensdorsal raphe nucleusmedicine.medical_treatmentstriatumPharmacologySmoking cessationSettore BIO/09 - FisiologiaSerotonin -- Receptorslcsh:ChemistryNicotineRats Sprague-DawleyNicotine addiction -- Treatment0302 clinical medicine5-HT2Asubstantia nigra pars compactaReceptor Serotonin 5-HT2Adentate gyruslcsh:QH301-705.5Spectroscopymedia_commonNeuronsGeneral MedicineDorsal raphe nucleu5-HT<sub>2C</sub>RComputer Science ApplicationsVentral tegmental areamedicine.anatomical_structuredepressionaddiction5-HT2CR; Addiction; Dentate gyrus; Depression; Dorsal raphe nucleus; Medial prefrontal cortex; Nucleus accumbens; Striatum; Substantia nigra pars compacta; Ventral tegmental area; Animals; Habenula; Male; Neurons; Nicotine; Rats; Rats Sprague-Dawley; Receptor Serotonin 5-HT2A; SerotoninReceptormedicine.drugAgonistNicotineSerotoninmedicine.drug_classmedia_common.quotation_subjectCentral nervous systemventral tegmental area substantia nigra pars compactaventral tegmental areaNucleus accumbensDentate gyruCatalysisArticleInorganic Chemistry03 medical and health sciencesmental disordersmedicineAnimalsNucleus accumbenPhysical and Theoretical ChemistryMolecular BiologyHabenulabusiness.industryAddictionOrganic ChemistryRats5-HT2CR030104 developmental biologylcsh:Biology (General)lcsh:QD1-999Smoking cessationSprague-DawleySerotoninbusinessReceptors Serotonin 5-HT2030217 neurology & neurosurgerymedial prefrontal cortex
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Monocyte preseeding leads to an increased implant bed vascularization of biphasic calcium phosphate bone substitutes via vessel maturation

2016

The present study analyzes the influence of the addition of monocytes to a biphasic bone substitute with two granule sizes (400-700 μm and 500-1000 μm). The majority of the added monocytes was detectable as mononuclear cells, while also low amounts of (chimeric) multinucleated giant cells (MNGCs) were found. No increase in the total number of MNGCs was established, but a significantly increased percent vascularization. Altogether, the results show that the added monocytes become involved in the tissue response to a biomaterial without marked changes in the overall reaction. Monocyte addition enables an increased implant bed vascularization especially via induction of vessel maturation and, …

0301 basic medicineMaterials scienceBone substituteMonocyteGranule (cell biology)Metals and AlloysBiomedical EngineeringBiomaterialBiphasic calcium phosphatePeripheral blood mononuclear cellCell biologyBiomaterials03 medical and health sciences030104 developmental biology0302 clinical medicinemedicine.anatomical_structureGiant cell030220 oncology & carcinogenesisImmunologyCeramics and CompositesmedicineImplantJournal of Biomedical Materials Research Part A
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Surface structure promoted high-yield growth and magnetotransport properties of Bi2Se3 nanoribbons

2019

AbstractIn the present work, a catalyst-free physical vapour deposition method is used to synthesize high yield of Bi2Se3 nanoribbons. By replacing standard glass or quartz substrates with aluminium covered with ultrathin porous anodized aluminium oxide (AAO), the number of synthesized nanoribbons per unit area can be increased by 20–100 times. The mechanisms of formation and yield of the nanoribbons synthesized on AAO substrates having different arrangement and size of pores are analysed and discussed. It is shown that the yield and average length of the nanoribbons can base tuned by adjustment of the synthesis parameters. Analysis of magnetotransport measurements for the individual Bi2Se3…

0301 basic medicineMaterials scienceYield (engineering)Nanowirelcsh:Medicinechemistry.chemical_elementSubstrate (electronics)TOPOLOGICAL INSULATORArticleInorganic Chemistry03 medical and health scienceschemistry.chemical_compound0302 clinical medicineBI2TE3AluminiumMaterials ChemistryElectronic devices[CHIM]Chemical SciencesTopological insulatorsDEPOSITIONlcsh:ScienceNANOWIRESurface statesMultidisciplinaryAnodizingPOROUS ALUMINAlcsh:ROrganic ChemistrySynthesis and processingCondensed Matter PhysicsARRAYS030104 developmental biologychemistryChemical engineeringAluminium oxidelcsh:QLayer (electronics)030217 neurology & neurosurgeryScientific Reports
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Mucoadhesive solid lipid microparticles for controlled release of a corticosteroid in the chronic obstructive pulmonary disease treatment.

2017

Therapeutic efficacy of inhaled drugs is limited by rapid clearance from the site of action due to absorption into systemic circulation or metabolic degradation by alveolar macrophages. Drug delivery systems offer new solutions to clinical problems especially in the treatment of pulmonary diseases. In particular, Solid Lipid Microparticles (SLM) in the range of 3-5 µm are suggested as systems for delivery of therapeutics to the lung as, because of their size, they are able to deposit into secondary bronchi, avoiding systemic absorption typical of alveolar regions. Here, we describe two novel different SLMs prepared with chitosan and alginate for sustained release of fluticasone propionate (…

0301 basic medicineMedicine (miscellaneous)Biocompatible Materials02 engineering and technologyPharmacologymedicine.disease_causeChitosanPulmonary Disease Chronic Obstructivechemistry.chemical_compoundDrug StabilityGlucuronic AcidAdrenal Cortex HormonesMucoadhesive Solid Lipid Nanoparticles (SLMs);Aerodynamic diameter;Chronic obstructive pulmonary disease (COPD)General Materials Sciencechronic obstructive pulmonary disease (COPD)LungChromatography High Pressure LiquidDrug CarriersHexuronic Acidsaerodynamic diameter; chronic obstructive pulmonary disease (COPD); mucoadhesive solid lipid microparticles (SLMs)021001 nanoscience & nanotechnologyLipidsControlled releasemucoadhesive solid lipid microparticles (SLMs)Microspheresmedicine.anatomical_structureDrug deliveryCorticosteroid0210 nano-technologymedicine.drugBiocompatibilityAlginatesCell SurvivalSurface Propertiesmedicine.drug_classBiomedical EngineeringBioengineeringDevelopmentFluticasone propionate03 medical and health sciencesAdministration InhalationmedicineHumansParticle Sizeaerodynamic diameterChitosanLungbusiness.industryEpithelial CellsDrug LiberationOxidative Stress030104 developmental biologychemistryDelayed-Action PreparationsImmunologyMicroscopy Electron ScanningFluticasonebusinessOxidative stress
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Molecular Mechanism of Inhibition of DNA Methylation by Zebularine

2017

In this work, we have analyzed the molecular mechanism of inhibition of a C5-DNA methyltransferase by zebularine using classical and QM/MM simulations. We found that the reaction proceeds with the addition of an unprotonated cysteine to the C6 position of the ring followed by methyl transfer to the C5 position. However, while the first step is reversible and presents a moderate free-energy barrier, the second step presents a large free-energy barrier, preventing the formation of the methylated complex. This mechanistic proposal agrees with recent experimental observations that point to the formation of a reversible covalent complex between DNA containing zebularine and methyltransferases. T…

0301 basic medicineMethyltransferaseStereochemistrySubstrate (chemistry)General ChemistryCatalysisQM/MM03 medical and health scienceschemistry.chemical_compound030104 developmental biologychemistryZebularineCovalent bondDNACytosineCysteineACS Catalysis
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The diagnosis of chronic endometritis in infertile asymptomatic women: a comparative study of histology, microbial cultures, hysteroscopy, and molecu…

2017

Background Chronic endometritis is a persistent inflammation of the endometrial mucosa caused by bacterial pathogens such as Enterobacteriaceae, Enterococcus, Streptococcus, Staphylococcus, Mycoplasma, and Ureaplasma. Although chronic endometritis can be asymptomatic, it is found in up to 40% of infertile patients and is responsible for repeated implantation failure and recurrent miscarriage. Diagnosis of chronic endometritis is based on hysteroscopy of the uterine cavity, endometrial biopsy with plasma cells being identified histologically, while specific treatment is determined based on microbial culture. However, not all microorganisms implicated are easily or readily culturable needing …

0301 basic medicineMicrobiological cultureBiopsyStaphylococcusChlamydia trachomatismedicine.disease_causeGastroenterologyUreaplasmaEndometriumGonorrhea0302 clinical medicineGardnerella vaginalisPathology MolecularAsymptomatic InfectionsEscherichia coli Infections030219 obstetrics & reproductive medicinebiologymedicine.diagnostic_testObstetrics and GynecologyHigh-Throughput Nucleotide SequencingBacterial InfectionsMiddle AgedStaphylococcal InfectionsGardnerella vaginalisMycoplasma hominisKlebsiella pneumoniaeFemaleEndometritisInfertility FemaleAdultDNA Bacterialmedicine.medical_specialtyPlasma CellsMycoplasma hominisHysteroscopyReal-Time Polymerase Chain ReactionSensitivity and Specificity03 medical and health sciencesYoung AdultMolecular microbiologyInternal medicineCulture TechniquesStreptococcal InfectionsmedicineEscherichia coliHumansMycoplasma InfectionsGram-Positive Bacterial Infectionsbusiness.industryStreptococcusSequence Analysis DNAChlamydia Infectionsbiology.organism_classificationNeisseria gonorrhoeaeKlebsiella Infections030104 developmental biologyChronic DiseasebusinessChronic EndometritisChlamydia trachomatisEnterococcusEndometrial biopsyAmerican journal of obstetrics and gynecology
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