Search results for "Bacteria"
showing 10 items of 4919 documents
Supramolecular Hydro- and Ionogels: A Study of Their Properties and Antibacterial Activity.
2017
Diimidazolium-based organic salts, bearing peptides or amino acids as anions have been synthesised and tested for their gelling ability in biocompatible solvents. These low molecular weight salts were successfully used as gelators in phosphate buffered saline (PBS) solution and ionic liquids. Then, the properties of the obtained soft materials were analysed in terms of melting temperature and gel strength as accounted for by rheological investigations. The gel-phase formation was studied by using UV/Vis and resonance light scattering measurements, whereas the morphology of the soft materials was analysed by using polarised optical microscopy and scanning electron microscopy. To get informat…
Advanced Strategies for Food-Grade Protein Production: A New
2019
Food-grade production of recombinant proteins in Gram-positive bacteria, especially in LAB (i.e., Lactococcus, Lactobacillus, and Streptococcus), is of great interest in the areas of recombinant enzyme production, industrial food fermentation, gene and metabolic engineering, as well as antigen delivery for oral vaccination. Food-grade expression relies on hosts generally considered as safe organisms and on clone selection not dependent on antibiotic markers, which limit the overall DNA manipulation workflow, as it can be carried out only in the expression host and not in E. coli. Moreover, many commercial expression vectors lack useful elements for protein purification. We constructed a “sh…
Midbiotics : conjugative plasmids for genetic engineering of natural gut flora
2019
ABSTRACTThe possibility to modify gut bacterial flora has become an important goal, and various approaches are used to achieve desirable communities. However, the genetic engineering of existing microbes in the gut, which are already compatible with the rest of the community and host immune system, has not received much attention. Here, we discuss and experimentally evaluate the possibility to use modified and mobilizable CRISPR-Cas9-endocing plasmid as a tool to induce changes in bacterial communities. This plasmid system (briefly midbiotic) is delivered from bacterial vector into target bacteria via conjugation. Compared to, for example, bacteriophage-based applications, the benefits of c…
Efficacy of different concentrations of sodium hypochlorite and chlorhexidine in disinfection of contaminated Resilon cones.
2011
Objectives: The aim of this study was to evaluate the effectiveness of different concentrations of Chlorhexidine (CHX) and sodium hypochlorite (NaOCl) in disinfecting contaminated Resilon cones within one minute. Study design: Fifty Resilon cones were divided into seven experimental groups and three control groups of 5 cones each. The cones of experimental groups were contaminated with Entrococcus faecalis and subsequently disinfected with different concentrations of NaOCl or CHX. The cones were then transferred into glass tubes containing thioglycollate media and incubated for 7 days. The tubes were examined for turbidity every 24 hours, and if bacterial growth occurred, samples were plate…
Silver nanoparticles in resin luting cements: antibacterial and physiochemical properties
2016
Background Silver has a long history of use in medicine as an antimicrobial and anti-inflammatory agent. Silver nanoparticles (NAg) offer the possibility to control the formation oral biofilms through the use of nanoparticles with biocidal, anti-adhesive, and delivery abilities. This study aims to evaluate the antibacterial effect of resin luting cements with and without NAg, and their influence on color, sorption and solubility. Material and methods NAg were incorporated to two dual-cured resin cements (RelyX ARC (RA) color A1 and RelyX U200 (RU) color A2) in two concentrations (0.05% and 0.07%, in weight), obtaining six experimental groups. Disc specimens (1x6mm) were obtained to verify t…
Additional file 2: Table S1. of Coinfection outcome in an opportunistic pathogen depends on the inter-strain interactions
2017
Effect of the treatment (single or coinfection) on the growth parameters of Flavobacterium columnare. (DOC 29Â kb)
of A multilocus sequence analysis scheme for characterization of Flavobacterium columnare isolates
2019
of A multilocus sequence analysis scheme for characterization of Flavobacterium columnare isolates
of A multilocus sequence analysis scheme for characterization of Flavobacterium columnare isolates
2019
of A multilocus sequence analysis scheme for characterization of Flavobacterium columnare isolates
of A multilocus sequence analysis scheme for characterization of Flavobacterium columnare isolates
2019
of A multilocus sequence analysis scheme for characterization of Flavobacterium columnare isolates
of A multilocus sequence analysis scheme for characterization of Flavobacterium columnare isolates
2019
of A multilocus sequence analysis scheme for characterization of Flavobacterium columnare isolates