Search results for "Bacterium"

Prevalence of Mycobacterium bovis in agroecosystems : analysis of potential environmental reservoirs (soil, fresh water, soil fauna and aquatic fauna…

2016

Bovine tuberculosis is an infectious disease caused by Mycobacterium bovis. This disease affects cattle, and many species of domestic and wild mammals, and humans. The circulation of the bacteria in various multi-host systems promotes the maintenance of the disease and the contamination of cattle in the vicinity. Beside direct transmission of the bacteria through the respiratory route, indirect transmission, through inhalation or ingestion of environmental matrices contaminated by an infected animal excretory, is suspected in several countries. Environmental contamination with M. bovis appears to be a crucial factor in the persistence of the infection in multi-host systems. In Côte d'Or, a …

Antibiotrophie : une fonction émergente favorisant l’implantation des bactéries antibiorésistantes dans les sols

2021

National audience; La fonction antibiotrophique, permettant l’utilisation d’antibiotiques comme source nutritive, arécemment été découverte chez des bactéries antibiorésistantes environnementales. Pourvérifier si leur avantage sélectif en environnements pollués s’en trouvait augmenté, nous avonsconduit une expérience en microcosmes dans laquelle Microbacterium sp. C448 (C448),présentant des capacités de résistance et de dégradation vis-à-vis des sulfamides, a été inoculéedans quatre sols supplémentés ou non par l’antibiotique sulfaméthazine (SMZ) et/ou du lisier.Après un mois d’incubation, C448 n’a été détectée que dans les sols traités avec la SMZ,indiquant une faible compétitivité de la s…

Selection of probiotic strains and improving the survival and functionality of an academic strain, Bifidobacterium bifidum, by changing the redox pot…

2012

The aim of this work was to select rationally a probiotic strain by setting up a screening method as well as study and understand the impact of the redox potential (Eh) and gas bubbling on the survival of Bifidobacterium bifidum in a fermented dairy product. In a first time, we have developed selection techniques of probiotic bacteria on the criteria of viability / vitality (flow cytometry analysis) and on the criteria of functionality (antioxidant). We were able to select strains of industrial interest as well as an academic strain, Bifidobacterium bifidum.Secondly, we have studied effect of modifying redox potential by gas bubbling on the survival of B. bifidum in a fermented dairy produc…

A mathematical method for determining genome divergence and species delineation using AFLP.

2002

The delineation of bacterial species is presently achieved using direct DNA-DNA relatedness studies of whole genomes. It would be helpful to obtain the same genomically based delineation by indirect methods, provided that descriptions of individual genome composition of bacterial genomes are obtained and included in species descriptions. The amplified fragment length polymorphism (AFLP) technique could provide the necessary data if the nucleotides involved in restriction and amplification are fundamental to the description of genomic divergences. Firstly, in order to verify that AFLP analysis permits a realistic exploration of bacterial genome composition, the strong correspondence between …

Vers la validation fonctionnelle du gène WEE1 dans l’acquisition de la résistance au stress abiotique chez des protéagineux

2017

Détection culturale et moléculaire de Mycobacterium bovis, l'agent de la tuberculose bovine, dans les matrices environnementales

2013

Etude comparative de deux méthodes de transformation et de deux milieux de culture chez Agrobacterium tumefaciens souche LBA4404

2013

Rapport de stage de master 1 BAP GEAPSI

Transcriptomic and proteomic responses of Microbacterium sp. C448 exposed to sulfamethazine antibiotic

2022

International audience; The Microbacterium sp. C448 was isolated from a soil regularly exposed to sulfamethazine(SMZ), for its ability to partly mineralise this antibiotic and other related sulfonamides.The aim of our study was to explore its metabolic adaptation towards exposure to SMZenvironmental (10 mg/L) and medicinal (250 mg/L) concentrations. Its transcriptomic andproteomic responses were analysed by focusing on the degradation regulon (sad genes) andresistance genes (folP and sul1).The transcriptomic and proteomic results were essentially congruent whatever theconcentrations tested. In culture conditions, exposure to the highest concentration of SMZ led tothe highest sad expression …

Discovering lactic acid bacteria by genomics

2002

International audience; This review summarizes a collection of lactic acid bacteria that are now undergoing genomic sequencing and analysis. Summaries are presented on twenty different species, with each overview discussing the organisms fundamental and practical significance, environmental habitat, and its role in fermentation, bioprocessing, or probiotics. For those projects where genome sequence data were available by March 2002, summaries include a listing of key statistics and interesting genomic features. These efforts will revolutionize our molecular view of Gram-positive bacteria, as up to 15 genomes from the low GC content lactic acid bacteria are expected to be available in the pu…

Immunization of rainbow trout, Oncorhynchus mykiss (Walbaum), with a low molecular mass fraction isolated from Flavobacterium psychrophilum.

2008

Flavobacterium psychrophilum, the causative agent of rainbow trout fry syndrome has become a widespread fish pathogen in freshwater aquaculture worldwide. In this study, a low molecular mass fraction (P25-33), with an approximate weight of 25-33 kDa, was identified among F. psychrophilum strains in an immunoblotting analysis with anti-F. psychrophilum sera. The immunogenic efficacy of the isolated and extracted P25-33 was investigated in two intraperitoneal immunization trials with rainbow trout, Oncorhynchus mykiss (Walbaum). The first trial included immunizations using P25-33 with Freund's complete adjuvant (FCA) and the second trial included immunizations using P25-33, formalin-inactivat…