Search results for "Base"

showing 10 items of 8362 documents

Functional comparison of bacteria from the human gut and closely related non-gut bacteria reveals the importance of conjugation and a paucity of moti…

2016

International audience; The human GI tract is a complex and still poorly understood environment, inhabited by one of the densest microbial communities on earth. The gut microbiota is shaped by millennia of evolution to co-exist with the host in commensal or symbiotic relationships. Members of the gut microbiota perform specific molecular functions important in the human gut environment. This can be illustrated by the presence of a highly expanded repertoire of proteins involved in carbohydrate metabolism, in phase with the large diversity of polysaccharides originating from the diet or from the host itself that can be encountered in this environment. In order to identify other bacterial fun…

0301 basic medicine[SDV]Life Sciences [q-bio]lcsh:MedicineGut floraPathology and Laboratory Medicinemedicine.disease_causeBiochemistryDatabase and Informatics MethodsRNA Ribosomal 16SMedicine and Health SciencesDNA metabolismlcsh:SciencePhylogenyProtein MetabolismClostridium BotulinumMultidisciplinarybiologyChemotaxisGastrointestinal Microbiomedigestive oral and skin physiologyHuman microbiomeGenomicsBacterial Physiological PhenomenaGenomic DatabasesAdaptation PhysiologicalBacterial PathogensNucleic acidsMedical MicrobiologyConjugation GeneticPathogensBacteroides thetaiotaomicronResearch ArticleCell PhysiologyBacterial Physiological PhenomenaResearch and Analysis MethodsBiosynthesisMicrobiologydigestive systemMicrobiology03 medical and health sciencesBacterial ProteinsGeneticsmedicineHumansMicrobial PathogensEscherichia coliClostridiumBacteria030102 biochemistry & molecular biologyGut Bacterialcsh:ROrganismsBiology and Life SciencesComputational BiologyChemotaxisCell BiologyDNAGenome Analysisbiology.organism_classificationGastrointestinal MicrobiomeCell MetabolismBiological DatabasesMetabolism030104 developmental biologyEvolutionary biologylcsh:QGenome BacterialBacteria
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Age‐related ultrastructural changes of the basement membrane in the mouse blood‐brain barrier

2018

Abstract The blood‐brain barrier (BBB) is essential for a functional neurovascular unit. Most studies focused on the cells forming the BBB, but very few studied the basement membrane (BM) of brain capillaries in ageing. We used transmission electron microscopy and electron tomography to investigate the BM of the BBB in ageing C57BL/6J mice. The thickness of the BM of the BBB from 24‐month‐old mice was double as compared with that of 6‐month‐old mice (107 nm vs 56 nm). The aged BBB showed lipid droplets gathering within the BM which further increased its thickness (up to 572 nm) and altered its structure. The lipids appeared to accumulate toward the glial side of the BM. Electron tomography …

0301 basic medicineblood‐brain barrierAgingElectron Microscope TomographyMyocytes Smooth Muscleelectron tomographyBlood–brain barrierMuscle Smooth Vascular03 medical and health sciencesMice0302 clinical medicineMicroscopy Electron TransmissionLipid dropletmedicineAnimalsBasement membraneChemistryNeurodegenerationBrainLipid metabolismBiological TransportCell BiologyOriginal ArticlesLipid Dropletsmedicine.diseaseLipid Metabolismbasement membraneCell biologyCapillariesMice Inbred C57BL030104 developmental biologymedicine.anatomical_structureElectron tomographyAgeingageingBlood-Brain Barrier030220 oncology & carcinogenesisAstrocytesUltrastructureMolecular MedicineOriginal ArticleNeuroglia
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Elderly Onset Celiac Disease: A Narrative Review.

2016

Celiac sprue is a chronic disease, which usually occurs in children and young adults. However, it can develop in any age group, and the prevalence is increasing even in the elderly population. The atypical patterns of clinical presentation in this age group sometimes can cause a delay in diagnosis. Given the lower sensitivity and specificity of serological tests in the aged population, clinical suspect often arises in the presence of complications (autoimmune disorders, fractures, and finally, malignancy) and must be supported by endoscopic and imaging tools. In this review, we highlight the incidence and prevalence of celiac disease in the elderly, the patterns of clinical presentation, d…

0301 basic medicinecardiovascular riskPediatricsmedicine.medical_specialtyEvidence-based practiceSettore MED/09 - Medicina InternaDiseaseReviewMalignancyBioinformaticselderlySprue03 medical and health sciences0302 clinical medicinemedicineYoung adultlcsh:RC799-869cardiovascular risk; celiac disease; elderly; presentationsSettore MED/12 - Gastroenterologiapresentationsbusiness.industryIncidence (epidemiology)Gastroenterologymedicine.disease030104 developmental biologyInformatics030211 gastroenterology & hepatologylcsh:Diseases of the digestive system. GastroenterologyPresentation (obstetrics)businessceliac diseaseClinical medicine insights. Gastroenterology
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SANS (USH1G) Molecularly Links the Human Usher Syndrome Protein Network to the Intraflagellar Transport Module by Direct Binding to IFT-B Proteins.

2019

The human Usher syndrome (USH) is a retinal ciliopathy, characterized by profound congenital deafness, variable vestibular dysfunction and pre-pubertal onset of retinitis pigmentosa. In the effected sensory cells, USH protein networks are assumed to function in ciliary transport processes. The USH1G protein SANS is a scaffold of the ciliary/periciliary USH protein network of photoreceptor cells. Moreover, SANS is associated with microtubules, the transport routes for protein delivery toward the cilium. To enlighten the role of SANS in ciliary transport processes, we aimed to identify transport related proteins associated with SANS. The intraflagellar transport (IFT) system is a conserved me…

0301 basic medicineciliary transportIFTPhotoreceptor cell570 Life sciences03 medical and health sciencesCell and Developmental Biology0302 clinical medicineprimary ciliaMicrotubuleIntraflagellar transportRetinitis pigmentosamedicinephotoreceptor celllcsh:QH301-705.5USH interactomeOriginal ResearchChemistryCiliumCell Biologymedicine.diseaseCell biologyCiliopathy030104 developmental biologymedicine.anatomical_structureciliopathylcsh:Biology (General)030220 oncology & carcinogenesisUSH1GAnkyrin repeatsense organsCiliary baseUsher syndrome570 BiowissenschaftenDevelopmental BiologyFrontiers in cell and developmental biology
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The still under-investigated role of cognitive deficits in PML diagnosis

2017

Background: Despite cognitive deficits frequently represent the first clinical manifestations of Progressive Multifocal Leukoencephalopathy (PML) in Natalizumab-treated MS patients, the importance of cognitive deficits in PML diagnosis is still under-investigated. The aim of the current study is to investigate the cognitive deficits at PML diagnosis in a group of Italian patients with PML. Methods: Thirty-four PML patients were included in the study. The demographic and clinical data, the lesion load and localization, and the longitudinal clinical course was compared between patients with (n = 13) and without (n = 15) cognitive deficit upon PML suspicion (the remaining six patients were asy…

0301 basic medicinecognitionmedicine.medical_specialtyPediatricscognition; italian database; natalizumab; neuropsychological impairment; progressive multifocal leukoencephalopathy; neurology (clinical); neurology; immunology; immunology and allergy; natalizumab; cognition; neuropsychological impairment; italian databaseNeurologySettore MED/17 - Malattie InfettiveAsymptomaticApraxiaprogressive multifocal leukoencephalopathyimmunology03 medical and health sciences0302 clinical medicinenatalizumabitalian databasemedicineDementiaimmunology and allergyPsychiatryCognitive deficitneurology (clinical)Progressive multifocal leukoencephalopathyneurologyNeuropsychologyCognitionProgressive multifocal leukoencephalopathy Natalizumab Cognition Neuropsychological impairment Italian databasemedicine.disease030104 developmental biologyCognition; Italian database; Natalizumab; Neuropsychological impairment; Progressive multifocal leukoencephalopathyCognition Italian database Natalizumab Neuropsychological impairment Progressive multifocal leukoencephalopathyCognition; Italian database; Natalizumab; Neuropsychological impairment; Progressive multifocal leukoencephalopathy; Immunology and Allergy; Immunology; Neurology; Neurology (clinical)Settore MED/26 - Neurologiamedicine.symptomPsychologyneuropsychological impairment030217 neurology & neurosurgery
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Coincident Activation of Glutamate Receptors Enhances GABAA Receptor-Induced Ionic Plasticity of the Intracellular Cl−-Concentration in Dissociated N…

2019

Massive activation of γ-amino butyric acid A (GABAA) receptors during pathophysiological activity induces an increase in the intracellular Cl−-concentration ([Cl−]i), which is sufficient to render GABAergic responses excitatory. However, to what extent physiological levels of GABAergic activity can influence [Cl−]i is not known. Aim of the present study is to reveal whether moderate activation of GABAA receptors mediates functionally relevant [Cl−]i changes and whether these changes can be augmented by coincident glutamatergic activity. To address these questions, we used whole-cell patch-clamp recordings from cultured cortical neurons [at days in vitro (DIV) 6–22] to determine changes in t…

0301 basic medicinedissociated cell cultureKCC2StimulationGABA(A) receptorsreversal potentiallcsh:RC321-57103 medical and health sciencesCellular and Molecular NeuroscienceGlutamatergicchemistry.chemical_compound0302 clinical medicinerheobaseReversal potentialionic plasticitylcsh:Neurosciences. Biological psychiatry. NeuropsychiatrymouseOriginal ResearchChemistryGABAA receptorGlutamate receptor030104 developmental biologyMuscimolCellular NeuroscienceBiophysicsExcitatory postsynaptic potentialCl−-homeostasisGABAergic030217 neurology & neurosurgeryFrontiers in Cellular Neuroscience
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In.Line: A Navigation Game for Visually Impaired People

2017

Part 3: Serious Games; International audience; In.line is a novel game based on a navigation system, called ARIANNA (pAth Recognition for Indoor Assisted NavigatioN with Augmented perception, [1]), primarily designed for visually impaired people permitting to navigate and find some points of interests in an indoor and outdoor environment by following a path painted or stuck on the floor. The aim of the game is twofold: (1) let the users learn and familiarize with the system, (2) improve blind people spatial skills to let them learn and acquire an allocentric spatial representation. The impact stands in the possibility of enhancing the social inclusion of a large part of the society that is …

0301 basic medicineeducation.field_of_studyComputer scienceVisually impairedmedia_common.quotation_subjectPopulationNavigation systemGameNavigation03 medical and health sciences030104 developmental biology0302 clinical medicineAssistive technologyHuman–computer interactionPerceptionLine (geometry)Path (graph theory)Spatial representationGame based[INFO]Computer Science [cs]Visually impaired people10. No inequalityeducation030217 neurology & neurosurgerymedia_common
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Genome-wide association meta-analysis of corneal curvature identifies novel loci and shared genetic influences across axial length and refractive err…

2020

Corneal curvature, a highly heritable trait, is a key clinical endophenotype for myopia - a major cause of visual impairment and blindness in the world. Here we present a trans-ethnic meta-analysis of corneal curvature GWAS in 44,042 individuals of Caucasian and Asian with replication in 88,218 UK Biobank data. We identified 47 loci (of which 26 are novel), with population-specific signals as well as shared signals across ethnicities. Some identified variants showed precise scaling in corneal curvature and eye elongation (i.e. axial length) to maintain eyes in emmetropia (i.e. HDAC11/FBLN2 rs2630445, RBP3 rs11204213); others exhibited association with myopia with little pleiotropic effects …

0301 basic medicinegenetic structuresMedicine (miscellaneous)EmmetropiaGenome-wide association studyVARIANTSGenome-wide association studiesSensory disorders Donders Center for Medical Neuroscience [Radboudumc 12]Cornea0302 clinical medicineRisk FactorsCorneaDatabases GeneticMULTIPLEMyopiaGene Regulatory NetworksEXPRESSION PATTERNS10. No inequalitylcsh:QH301-705.5POPULATIONGeneticseducation.field_of_studymedicine.diagnostic_testHERITABILITYCorneal DiseasesAsian Continental Ancestry Group ; Axial Length Eye ; Cornea ; Corneal Topography ; Databases Genetic ; European Continental Ancestry Group ; Gene Regulatory Networks ; Genetic Loci ; Genetic Predisposition to Disease ; Genome-Wide Association Study ; Humans ; Myopia ; Phenotype ; Polymorphism Single Nucleotide ; Refractometry ; Risk Assessment ; Risk FactorsCorneal topographyEYE SIZE3. Good healthAxial Length EyePhenotypemedicine.anatomical_structureGeneral Agricultural and Biological SciencesExtracellular matrix organizationKeratoconusCorneal diseasesPopulationBiologyPolymorphism Single NucleotideRisk AssessmentArticleWhite PeopleGeneral Biochemistry Genetics and Molecular BiologyOCULAR COMPONENT DIMENSIONS03 medical and health sciencesSPHERICAL EQUIVALENTAsian PeoplemedicineHumansGenetic Predisposition to DiseaseKERATOCONUS3125 Otorhinolaryngology ophthalmologyeducationCorneal Topographymedicine.diseaseCOLLAGENeye diseasesRefractometry030104 developmental biologylcsh:Biology (General)Genetic LociRE3111 Biomedicinesense organs030217 neurology & neurosurgeryGenome-Wide Association StudyCommunications Biology
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Cost-Effective, Safe, and Personalized Cell Therapy for Critical Limb Ischemia in Type 2 Diabetes Mellitus.

2019

The Collaborative Working Group “Noma Project Team”.

0301 basic medicinelcsh:Immunologic diseases. Allergycritical limb ischemiaCell typecost-effectivecell-based therapyImmunologyBioinformaticsCell therapy03 medical and health sciences0302 clinical medicineImmunology and AllergyMedicineOriginal Researchclinical trialsdiabetesbusiness.industryMesenchymal stem cellType 2 Diabetes MellitusCritical limb ischemiacellular medicamentsClinical trial030104 developmental biologyStem cellmedicine.symptombusinesslcsh:RC581-607030215 immunologyAdult stem cell
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The shell of the invasive bivalve species Dreissena polymorpha: biochemical, elemental and textural Investigations.

2016

28 pages; International audience; The zebra mussel Dreissena polymorpha is a well-established invasive model organism. Although extensively used in environmental sciences, virtually nothing is known of the molecular process of its shell calcification. By describing the microstructure, geochemistry and biochemistry/proteomics of the shell, the present study aims at promoting this species as a model organism in biomineralization studies, in order to establish a bridge with ecotoxicology, while sketching evolutionary conclusions. The shell of D. polymorpha exhibits the classical crossed-lamellar/complex crossed lamellar combination found in several heterodont bivalves, in addition to an extern…

0301 basic medicinelcsh:MedicineInvasive Species010501 environmental sciencesProteomicsEcotoxicology01 natural sciencesBiochemistrychemistry.chemical_compoundDatabase and Informatics MethodsMaterials PhysicsLectinsMusselslcsh:ScienceMicrostructureGel ElectrophoresisStainingMineralsMultidisciplinarybiologyOrganic CompoundsPhysicsMonosaccharidesBiological EvolutionEuropeChemistry[ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]Physical SciencesFranceSequence AnalysisResearch ArticleSilver StainingBivalvesMaterials ScienceShell (structure)CarbohydratesSequence DatabasesElectrophoretic StainingResearch and Analysis MethodsDreissenaDreissenaCoomassie Blue staining03 medical and health sciencesElectrophoretic TechniquesSpecies ColonizationAnimal Shells[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]Botany[SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular BiologyEcotoxicologyAnimals[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology14. Life underwaterShell calcificationMolecular Biology TechniquesSequencing Techniques[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/BiomaterialsMolecular Biology[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry Molecular Biology0105 earth and related environmental scienceslcsh:ROrganic ChemistryEcology and Environmental SciencesOrganismsChemical CompoundsBiology and Life SciencesProteinsMolluscs[ SDV.IB.BIO ] Life Sciences [q-bio]/Bioengineering/Biomaterialsbiology.organism_classificationInvertebrates030104 developmental biologyCalcium carbonateBiological DatabaseschemistrySpecimen Preparation and TreatmentZebra mussellcsh:QIntroduced SpeciesBiomineralization
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