Search results for "Begomovirus"

showing 3 items of 13 documents

Spread of tomato yellow leaf curl virus in Sicily: partial displacement of another geminivirus originally present.

2006

The geminivirus Tomato yellow leaf curl virus (TYLCV) was reported for the first time in Italy in 2002. We have followed its spread in Sicily, where Tomato yellow leaf curl Sardinia virus (TYLCSV), another tomato-infecting geminivirus, is endemic and has been causing severe crop losses since 1989. The presence of the two viruses was monitored in the main tomato growing area, the Ragusa province, analyzing samples with yellow leaf curling symptoms. At first (spring-summer 2002) both viruses were always found in mixed infections, but in 2003 and 2004 18-35% of plants were found infected by TYLCV alone and 8-28% by TYLCSV alone, with 41-69% carrying both viruses. TYLCV has spread quickly in th…

Veterinary medicinegeminivirusvirusesBegomovirusfungiinfectious clonespreadSettore AGR/12 - Patologia VegetaleVirulencefood and beveragesnucleotide sequencePlant ScienceHorticultureBiologybiology.organism_classificationVirologyVirusCropTYLCSVGeminiviridaeTomato yellow leaf curl virusAgronomy and Crop SciencegeminiviruSolanaceaeMixed infection
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The complete nucleotide sequence of an isolate of Tomato yellow leaf curl Sardinia virus found in Sicily

2010

Partial sequences of Tomato yellow leaf curl Sardinia virus (TYLCSV) derived from tomato samples collected in Sicily in 1999, 2002 and 2004 indicated the presence of a TYLCSV different from the one previously described as the Sic strain. Here, we report a complete DNA sequence that is classified as belonging to the TYLCSV type strain (Sar strain), confirming the co-existence in Sicily of virus populations of both strains. Moreover, comparisons between this new sequence and those of the two recombinants recently described in Sicily revealed unequivocally (99% identity) that their TYLCSV-derived portion originated from the Sar strain. © 2010 Springer-Verlag.

biologyStrain (chemistry)Base SequencefungiBegomovirusMolecular Sequence DataNucleic acid sequencefood and beveragesSettore AGR/12 - Patologia VegetaleGeneral Medicinebiology.organism_classificationVirologyDNA sequencingVirusSolanum lycopersicumPhylogeneticsVirologyPlant virusBegomovirusBotanyGeminiviridaeSicilyPhylogenyTYLCS ItalyPlant Diseases
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A single tube PCR assay for detecting viruses and their recombinants that cause tomato yellow leaf curl disease in Mediterranean basin

2007

Tomato yellow leaf curl disease (TYLCD) is well known in Mediterranean countries, where it has been causing severe losses in tomato crops for decades. Until recently, two viruses (with several isolates) in the genus Begomovirus, family Geminiviridae, have been associated with the epidemics: Tomato yellow leaf curl virus (TYLCV) and Tomato yellow leaf curl Sardinia virus (TYLCSV). However, recombinants between these, such as Tomato yellow leaf curl Malaga virus (TYLCMalV), are spreading, and new methods for detecting all viruses present in the region are needed. By considering all DNA sequences available of viruses causing TYLCD in the Mediterranean basin, a PCR/RFLP protocol was developed t…

virusestomatoPolymerase Chain ReactionViruslaw.inventionIntergenic regionSolanum lycopersicumlawVirologyGeminiviridaeTomato yellow leaf curl virusrecombinantPolymerase chain reactionPlant DiseasesbiologyMediterranean RegionfungiBegomovirusSettore AGR/12 - Patologia VegetaleTYLCDfood and beveragesmultiplex PCRbiology.organism_classificationVirologyPlant LeavesgeminivursBegomovirusLeaf curlRestriction fragment length polymorphism
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