Search results for "Bioaerosol"

Metaproteomic analysis of atmospheric aerosol samples.

2016

Metaproteomic analysis of air particulate matter provides information about the abundance and properties of bioaerosols in the atmosphere and their influence on climate and public health. We developed and applied efficient methods for the extraction and analysis of proteins from glass fiber filter samples of total, coarse, and fine particulate matter. Size exclusion chromatography was applied to remove matrix components, and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied for protein fractionation according to molecular size, followed by in-gel digestion and LC-MS/MS analysis of peptides using a hybrid Quadrupole-Orbitrap MS. Maxquant software and the Swiss-…

Bioaerosols in the Amazon rain forest: temporal variations and vertical profiles of Eukarya, Bacteria, and Archaea

2021

The Amazon rain forest plays a major role in global hydrological cycling, and biogenic aerosols are likely to influence the formation of clouds and precipitation. Information about the sources and altitude profiles of primary biological aerosol particles, however, is sparse. We used fluorescence in situ hybridization (FISH), a molecular biological staining technique largely unexplored in aerosol research, to investigate the sources and spatiotemporal distribution of Amazonian bioaerosols on the domain level. We found wet season bioaerosol number concentrations in the range of 1–5 × 105 m−3 accounting for > 70 % of the coarse mode aerosol. Eukaryotic and bacterial particles predominated, …

Biotechnology and Conservation of Cultural Heritage

2017

Over the past few decades, biotechnology has provided innovative techniques useful for diagnosing cultural heritage deterioration induced by microbiological systems and for defining efficient conservation/restoration strategies. Seen from this per- spective, the International Congress on Molecular Biology and Cultural Heritage held in Seville (Spain) in 2003 represents a milestone. This book comes from the experience gained in the last decade of basic and applied research, developed in the Laboratory of Biology and Biotechnology for Cultural Heritage (LaBBCH), within the framework of national and international research projects and in collaboration with other Italian and foreign research in…

BIOAEROSOL IN INDOOR ENVIRONMENTS FOR CONSERVATION OF CULTURAL HERITAGE: POTENTIAL RISKS FOR HISTORICAL-ARTISTIC MANIFACTS ANO HUMAN HEALTH

2014

An integrated approach was applied for the characterization of the biological airborne particulate in four Sicilian sites with peculiar structure, thermo-hygrometric and lighting parameters. We focalized the attention on: ,) Crypt in the Cathedral Treasury Museum (Palermo), an underground environment (lithic and stone artifact), daily visited by tourist and characterized by a reduced indoor-outdoor exchange; it) the Saints Cave in Licodia Eubea (Catania), a semi-confined environment (fresco), strongly influenced by the surrounding countryside and by a continuous airflow; iit) the Diocesan Historic Archive in Palermo, (documentary funds, IX-XXsec.), characterized by low air change rate and r…

Abundance of cellular material and proteins in the atmosphere.

2005

Suspended atmospheric particles play a crucial role in any global climate scenario: They can both enforce and suppress radiative forcing. In developing climate modeling further, a deeper understanding of atmospheric aerosol is needed. Because of extreme local and temporal variations, proper incorporation of aerosols into models requires modeling of the aerosol itself. It turns out that cellular material and proteins compose up to 25% of the atmospheric aerosol. Consequently, the source strength of the biogenic aerosol in general must be corrected and should be estimated on the order of other major aerosol sources.

Développement et évaluation d'une méthode fondée sur la PCR temps réel pour la caractérisation des bioaérosols : application au groupe des actinomycè…

2013

Actinomycetes are ubiquitous bacteria and some can be potentially pathogen for Humans in the air of some working areas. It’s notably the case in composting plants where bacteria concentrations can reach high values. Workers exposure to these inhalable bioaerosols can be source of various diseases (hypersensitivity pneumonitis notably). Although this problem is admitted, bibliography reveals a lack of knowledge about risk assessment: currently, none global method for bioaerosols sampling and analysis is standardized. So much that neither dose-effects relationship for most of these bacteria, nor Threshold Limit Value exists. Traditional methods, that are used, have some drawbacks (concentrati…

Occupational hygiene in a Finnish drum composting plant

2005

Bioaerosols (microbes, dust and endotoxins) and volatile organic compounds (VOCs) were determined in the working air of a drum composting plant treating source-separated catering waste. Different composting activities at the Oulu drum composting plant take place in their own units separated by modular design and constructions. Important implication of this is that the control room is a relatively clean working environment and the risk of exposure to harmful factors is low. However, the number of viable airborne microbes was high both in the biowaste receiving hall and in the drum composting hall. The concentration (geometric average) of total microbes was 21.8 million pcs/m3 in the biowaste…

Bacterial bioaerosol in the warehousing area of food industry facility

2014

Branża spożywcza zwraca coraz większą uwagę na etap magazynowania surowców i produktów gotowych. Mikrobiologiczne zanieczyszczenie surowca czy produktu gotowego w wyniku błędów przy magazynowaniu może generować straty ekonomiczne dla przedsiębiorstwa. Celem pracy była ocena stanu bioaerozolu bakteriologicznego magazynów w wybranym zakładzie przemysłu spożywczego. Materiał badawczy stanowiła mikroflora bakteryjna wyizolowana z powietrza w strefie magazynowej wybranego zakładu przemysłu spożywczego. Analizie zostały poddane cztery pomieszczenia: magazyny produktów (MPI, MPII), magazyn techniczny (MT) oraz magazyn opakowań jednostkowych (MPJ). Badania wykonano metodą sedymentacyjną Kocha w trz…

Dobór metody oceny czystości mikrobiologicznej powietrza w zakładach przemysłu spożywczego

2016

Aerosol Health Effects from Molecular to Global Scales.

2017

Poor air quality is globally the largest environmental health risk. Epidemiological studies have uncovered clear relationships of gaseous pollutants and particulate matter (PM) with adverse health outcomes, including mortality by cardiovascular and respiratory diseases. Studies of health impacts by aerosols are highly multidisciplinary with a broad range of scales in space and time. We assess recent advances and future challenges regarding aerosol effects on health from molecular to global scales through epidemiological studies, field measurements, health-related properties of PM, and multiphase interactions of oxidants and PM upon respiratory deposition. Global modeling combined with epide…