Search results for "Biochemical engineering"
showing 10 items of 227 documents
CAPILLARY ELECTROPHORESIS | Crop-Protecting Agents
2007
Capillary eletrophoresis (CE) is becoming an advantageous technique for determining crop protecting agents as a result of its simplicity and high separation efficiency. Controversely, inappropriate limits of detection (LODs) and a lack of selective detectors restrict its applications. The present article provides a general idea of recent advances in off-column and on-column approaches to trace enrichment in analyzing real samples, and recapitulates the determination of crop-protecting agents by conventional CE as well as by emerging techniques, such as CE-laser induced fluorescence (LIF) or CE-mass spectrometry (MS). The usefulness of these approaches in food and environmental applications …
Analytical Techniques for Furosemide Determination
2006
Abstract Due to the clinical importance of furosemide, a large number of analytical procedures to detect the presence of this drug in pharmaceutical and physiological samples has been developed. In this manuscript, a review of the most frequent analytical techniques described to determine furosemide is presented. Special attention has been paid to spectrophotometric and chromatographic techniques, but also to relevant methods using capillary electrophoresis or flow‐injection analysis, as well as the detection modes coupled to these techniques. The review also focuses on the different degradation pathways of the drug and cautions to prevent it, otherwise rarely or confusedly mentioned in the…
Sample preparation methods for the determination of pesticides in foods using CE-UV/MS.
2010
Much progress has been made in pesticide analysis over the past decade, during this time hyphenated techniques involving highly efficient separation with sensitive detection have become the techniques of choice. Among these, methods based on separation with mass spectrometric detection have resulted in greater likelihood of identification and are acknowledged to be extremely useful and authoritative methods for the determination of pesticide residues but the inherent advantages of the use of CE as a separation technique are well-known and can be summarized as high separation efficiency, low analysis time, high resolution power, and low consumption of samples and reagents. Although UV is the…
Analytical Methods for Pesticide Residue Determination in Bee Products
2002
Monitoring pesticide residues in honey, wax, and bees helps to assess the potential risk of these products to consumer health and gives information on the pesticide treatments that have been used on the field crops surrounding the hives. The present review seeks to discuss the basic principles and recent developments in pesticide analysis in bee products and their application in monitoring programs. Consideration is given to extraction, cleanup, chromatographic separation, and detection techniques.
Aerobic Metabolism: Benefits from an Oxygenated World
2010
In the preceding chapter, we have emphasized the dangers that the advent of dioxygen presented to the existing anaerobic organisms, and the ways they evolved to deal with the problems. However, this is only part of the story and were it to have ended here, we and the world we know would not exist. What happened instead was quite remarkable; for life seized upon an opportunity presented by the presence of free dioxygen to become many-fold more efficient in extracting energy from foodstuffs. As we shall see, this aerobic, oxidative metabolism opened in turn a multitude of new opportunities for growth and diversification.
Innovative “Green” and Novel Strategies for the Extraction of Bioactive Added Value Compounds from Citrus Wastes—A Review
2017
Citrus is a major processed crop that results in large quantities of wastes and by-products rich in various bioactive compounds such as pectins, water soluble and insoluble antioxidants and essential oils. While some of those wastes are currently valorised by various technologies (yet most are discarded or used for feed), effective, non-toxic and profitable extraction strategies could further significantly promote the valorisation and provide both increased profits and high quality bioactives. The present review will describe and summarize the latest works concerning novel and greener methods for valorisation of citrus by-products. The outcomes and effectiveness of those technologies such a…
Applications of xylochemistry from laboratory to industrial scale
2020
Xylochemicals and their utilisation in total synthesis and industrial applications are highlighted in this review. This class of chemicals consists of bio-based molecules or building blocks derived from renewable feedstocks like plant-biomass, lignocellulose as well as waste-streams. The advantages over petroleum-based chemicals are presented with the aid of specific examples ranging from the total synthesis of natural products, relevant industrial applications to the exploitation of CO2 as a C1 feedstock. This review contributes to the trend of a “greener” and a more sustainable chemistry.
A multi-phase multi-objective genome-scale model shows diverse redox balance strategies in yeasts
2021
Yeasts constitute over 1500 species with great potential for biotechnology. Still, the yeastSaccharomyces cerevisiaedominates industrial applications and many alternative physiological capabilities of lesser-known yeasts are not being fully exploited. While comparative genomics receives substantial attention, little is known about yeasts’ metabolic specificity in batch cultures. Here we propose a multi-phase multi-objective dynamic genome-scale model of yeast batch cultures that describes the uptake of carbon and nitrogen sources and the production of primary and secondary metabolites. The model integrates a specific metabolic reconstruction, based on the consensus Yeast8, and a kinetic mod…
A multi-phase multi-objective dynamic genome-scale model shows different redox balancing among yeast species in fermentation
2021
ABSTRACTYeasts constitute over 1500 species with great potential for biotechnology. Still, the yeastSaccharomyces cerevisiaedominates industrial applications and many alternative physiological capabilities of lesser-known yeasts are not being fully exploited. While comparative genomics receives substantial attention, little is known about yeasts’ metabolic specificity in batch cultures. Here we propose a multi-phase multi-objective dynamic genome-scale model of yeast batch cultures that describes the uptake of carbon and nitrogen sources and the production of primary and secondary metabolites. The model integrates a specific metabolic reconstruction, based on the consensus Yeast8, and a kin…
Challenges in the determination of engineered nanomaterials in foods
2016
Detection, characterization, and quantification of engineering nanomaterials (ENMs) in foods is still a pending issue that needs to be tackle to protect consumers and to fix some related aspects (e.g. labelling or control). The global challenge for the analytical sciences is that ENMs are a new sort of analytes, involving both chemical (composition, mass and number concentration) and physical information (e.g. size, shape, aggregation). In this critical review, we evaluate and compare the procedures involved in the analytical methods and studies developed thus far for the identification and quantification of ENMs in food. We discuss advantages and limitation as well as prospects. We pointed…