Search results for "Biochemical"

showing 10 items of 870 documents

The Regulatory T Cell Family: Distinct Subsets and their Interrelations

2003

The immune system, a highly effective and dynamic cellular network, protects a host from pathogens. Therefore, the immune system must distinguish self from nonself structures, but also between harmful and innocuous foreign Ags to prevent nonessential and self-destructive immune responses. The

CD4-Positive T-LymphocytesImmunity CellularRegulatory T cellanimal diseasesImmunologyReceptors Interleukin-2chemical and pharmacologic phenomenaCell Communicationbiochemical phenomena metabolism and nutritionBiologyImmunity Innatemedicine.anatomical_structureImmune systemT-Lymphocyte SubsetsImmunityImmunologymedicineAnimalsHumansbacteriaImmunology and AllergyReceptorThe Journal of Immunology
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Oral microbiome in Proliferative Verrucous Leukoplakia exhibits loss of diversity and enrichment of pathogens.

2021

Abstract Objectives Oral microbiome plays an important role in oral diseases. Among them, proliferative verrucous leucoplakia (PVL) is an uncommon form of progressive multifocal leukoplakia with a worryingly rate of malignant transformation. Here, we aimed to characterize the oral microbiome of PVL patients and compare it with those of healthy controls. Material and methods Oral biopsies from ten PVL patients and five healthy individuals were obtained and used to compare their microbial communities. The sequence of the V3-V4 region of 16S rRNA gene was used as the taxonomic basis to estimate and analyze the composition and diversity of bacterial populations present in the samples. Results O…

Cancer ResearchBiopsymedicine.disease_causeCampylobacter jejuniRNA Ribosomal 16SmedicineProliferative verrucous leukoplakiaHumansEubacteriumLeukoplakiaMouthbiologyCampylobacterMicrobiotabiochemical phenomena metabolism and nutritionrespiratory systembacterial infections and mycosesbiology.organism_classificationmedicine.disease16S ribosomal RNAstomatognathic diseasesCell Transformation NeoplasticOncologyImmunologyOral MicrobiomeOral SurgeryLeukoplakia OralDysbiosisOral oncology
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Evolutionary convergence and nitrogen metabolism in Blattabacterium strain Bge, primary endosymbiont of the cockroach Blattella germanica.

2009

Bacterial endosymbionts of insects play a central role in upgrading the diet of their hosts. In certain cases, such as aphids and tsetse flies, endosymbionts complement the metabolic capacity of hosts living on nutrient-deficient diets, while the bacteria harbored by omnivorous carpenter ants are involved in nitrogen recycling. In this study, we describe the genome sequence and inferred metabolism of Blattabacterium strain Bge, the primary Flavobacteria endosymbiont of the omnivorous German cockroach Blattella germanica. Through comparative genomics with other insect endosymbionts and free-living Flavobacteria we reveal that Blattabacterium strain Bge shares the same distribution of functio…

Cancer Researchfood.ingredientlcsh:QH426-470NitrogenBlochmanniaZoologyCockroachesEvolution Molecular03 medical and health sciencesBlattabacteriumfoodSymbiosisEnterobacteriaceaePhylogeneticsAmmoniabiology.animalBotanyGeneticsAnimalsAmino AcidsSymbiosisMolecular BiologyGenetics (clinical)Ecology Evolution Behavior and SystematicsPhylogeny030304 developmental biology0303 health sciencesGerman cockroachCockroachbiologyPhylogenetic treeEvolutionary Biology/Evolutionary and Comparative Genetics030306 microbiologyAntsBacteroidetesfungiGenomicsbiochemical phenomena metabolism and nutritionbiology.organism_classificationGenetics and Genomics/Microbial Evolution and Genomicslcsh:GeneticsGenetics and Genomics/Genome ProjectsEvolutionary Biology/Microbial Evolution and GenomicsHost-Pathogen InteractionsBacteriaGenome BacterialMetabolic Networks and PathwaysResearch ArticlePLoS genetics
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[The activation of methionine for transmethylation in regenerating rat liver and hepatoma].

1958

The activity of methionine-activating enzyme has been measured in normal and regenerating rat liver and in hepatoma cells.

Cancer Researchmedicine.medical_specialtyCarcinoma HepatocellularMethionine metabolismBiochemical Phenomenachemistry.chemical_compoundMethionineInternal medicinemedicineAnimalschemistry.chemical_classificationMethionineHematologyChemistryLiver NeoplasmsGeneral Medicinedigestive system diseasesRatsLiver metabolismEnzymeOncologyBiochemistryLiverRat liverHepatoma cellTransmethylationZeitschrift fur Krebsforschung
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Differences in Kaposi sarcoma-associated herpesvirus-specific and herpesvirus-non-specific immune responses in classic Kaposi sarcoma cases and match…

2011

Kaposi sarcoma (KS) might develop because of incompetent immune responses, both non-specifically and specifically against the KS-associated herpesvirus (KSHV). Peripheral blood mononuclear cells from 15 classic (non-AIDS) KS cases, 13 KSHV seropositives (without KS) and 15 KSHV-seronegative controls were tested for interferon-γ T-cell (enzyme-linked immunospot [Elispot]) responses to KSHV-latency-associated nuclear antigen (LANA), KSHV-K8.1 and CMV/Epstein-Barr virus (EBV) peptide pools. The forearm and thigh of each participant was also tested for delayed-type hypersensitivity (DTH) against common recall antigens. Groups were compared with Fisher exact test and multinomial logistic regress…

Cancer ResearchvirusesT-LymphocytesEnzyme-Linked Immunosorbent AssayBiologyAntibodies ViralSettore MED/42 - Igiene Generale E ApplicataPeripheral blood mononuclear cellArticleInterferon-gammaViral ProteinsImmune systemAntigenInterferonmedicineHumansInterferon gammaHypersensitivity DelayedAntigens ViralSarcoma KaposiSicilyGlycoproteinsKSHV SicilyClassic Kaposi SarcomaELISPOTvirus diseasesNuclear ProteinsAntigens NuclearGeneral Medicinebiochemical phenomena metabolism and nutritionmedicine.diseaseVirologyImmunity InnateKaposi sarcoma; HHV8; ELISPOT; immune responseOncologyCase-Control StudiesImmunologyHerpesvirus 8 HumanLeukocytes MononuclearSarcomamedicine.drug
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Small but Powerful, the Primary Endosymbiont of Moss Bugs, Candidatus Evansia muelleri, Holds a Reduced Genome with Large Biosynthetic Capabilities

2014

International audience; Moss bugs (Coleorrhyncha: Peloridiidae) are members of the order Hemiptera, and like many hemipterans, they have symbiotic associations with intracellular bacteria to fulfill nutritional requirements resulting from their unbalanced diet. The primary endosymbiont of the moss bugs, Candidatus Evansia muelleri, is phylogenetically related to Candidatus Carsonella ruddii and Candidatus Portiera aleyrodidarum, primary endosymbionts of psyllids and whiteflies, respectively. In this work, we report the genome of Candidatus Evansia muelleri Xc1 from Xenophyes cascus, which is the only obligate endosymbiont present in the association. This endosymbiont possesses an extremely …

Candidatus Carsonella ruddiimutualism[SDV]Life Sciences [q-bio]GenomeEvolution MolecularHemiptera03 medical and health sciencesMicroscopy Electron TransmissionBotanyGeneticsAnimalsColeorrhynchaPeloridiidaeSymbiosisgenome reductionGenome sizePhylogenyEcology Evolution Behavior and Systematics030304 developmental biologyGene RearrangementGenetics0303 health sciencesbiology030306 microbiologyfungiGene rearrangementbiochemical phenomena metabolism and nutritionbiology.organism_classificationHemipterametabolic complementationHalomonadaceaeCandidatusbacteriaendosymbiontResearch Article
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Carbapenem-Susceptible OXA-23-Producing Proteus mirabilis in the French Community

2019

International audience; Nineteen Proteus mirabilis isolates producing the carbapenemase OXA-23 were recovered over a 2-year period in 19 French hospitalized patients, of whom 12 had community onset infections. The isolates exhibited a slightly reduced susceptibility to carbapenems. Whole-genome analysis revealed that all 19 isolates formed a cluster compared to 149 other P. mirabilis isolates. Because of its susceptibility to carbapenems, this clone may be misidentified as a penicillinase producer while it constitutes a reservoir of the OXA-23-encoding gene in the community.

CarbapenemHospitalized patientsspreadclonalityMicrobial Sensitivity Testsbeta-LactamasesEpidemiology and SurveillanceMicrobiology03 medical and health sciencescarbapenemasemedicinepolycyclic compoundsHumansPharmacology (medical)Proteus mirabilis030304 developmental biologyCommunity onsetPharmacology0303 health sciencesbiologyOXA-23030306 microbiologybiochemical phenomena metabolism and nutritionbiology.organism_classificationProteus mirabilisAnti-Bacterial AgentsInfectious DiseasesReduced susceptibility[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyCarbapenemsbacteriaFranceProteus Infectionsmedicine.drug
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An Update of the Evolving Epidemic of blaKPC Carrying Klebsiella pneumoniae in Sicily, Italy, 2014: Emergence of Multiple Non-ST258 Clones

2015

Background: In Italy, Klebsiella pneumoniae carbapenemase producing K. pneumoniae (KPC-Kp) strains are highly endemic and KPC producing CC258 is reported as the widely predominating clone. In Palermo, Italy, previous reports have confirmed this pattern. However, recent preliminary findings suggest that an epidemiological change is likely ongoing towards a polyclonal KPC-Kp spread. Here we present the results of molecular typing of 94 carbapenem non susceptible K. pneumoniae isolates detected during 2014 in the three different hospitals in Palermo, Italy. Methods and Results: Ninety-four consecutive, non replicate carbapenem non susceptible isolates were identified in the three largest acute…

CarbapenemKlebsiella pneumoniaelcsh:MedicineGene ExpressionDrug resistancePlasmidbeta-LactamaseDisease OutbreaksMolecular typingFluoroquinoloneDrug Resistance Multiple Bacterialpolycyclic compoundslcsh:ScienceCarbapenemMembrane ProteinDisease OutbreakMultidisciplinarybiologyMedicine (all)IncidenceHospitalsAnti-Bacterial AgentsElectrophoresis Gel Pulsed-FieldKlebsiella pneumoniaeItalyEpidemiological MonitoringHumanFluoroquinolonesPlasmidsResearch Articlemedicine.drugBacterial ProteinAminoglycosides; Anti-Bacterial Agents; Bacterial Proteins; Carbapenems; Clone Cells; Colistin; Drug Resistance Multiple Bacterial; Electrophoresis Gel Pulsed-Field; Epidemiological Monitoring; Fluoroquinolones; Gene Expression; Hospitals; Humans; Incidence; Italy; Klebsiella Infections; Klebsiella pneumoniae; Membrane Proteins; Multilocus Sequence Typing; Mutation; Plasmids; beta-Lactamases; Disease Outbreaks; Agricultural and Biological Sciences (all); Biochemistry Genetics and Molecular Biology (all); Medicine (all)beta-LactamasesMicrobiologyClone CellHospitalAntibiotic resistanceBacterial ProteinsAnti-Bacterial AgentmedicineHumansBiochemistry Genetics and Molecular Biology (all)AminoglycosideColistinlcsh:RMembrane ProteinsCarbapenemase producingbiochemical phenomena metabolism and nutritionbacterial infections and mycosesbiology.organism_classificationVirologyClone CellsKlebsiella InfectionsAminoglycosidesAgricultural and Biological Sciences (all)CarbapenemsMutationColistinMultilocus sequence typinglcsh:QKlebsiella InfectionMultilocus Sequence TypingPLOS ONE
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Carbon based nanomaterials for the detection of narrow therapeutic index pharmaceuticals

2020

Precise detection of important pharmaceuticals with narrow therapeutic index (NTI) is very critical as there is a small window between their effective dose and the doses at which the adverse reactions are very likely to appear. Regarding the fact that various pharmacokinetics will be plausible while considering pharmacogenetic factors and also differences between generic and brand name drugs, accurate detection of NTI will be more important. Current routine analytical techniques suffer from many drawbacks while using novel biosensors can bring up many advantages including fast detection, accuracy, low cost with simple and repeatable measurements. Recently the well-known carbon Nano-allotrop…

Carbon nanostructuresBrand namesNanotubes CarbonChemistrytechnology industry and agricultureBiosensing TechniquesNanostructuresAnalytical ChemistryTherapeutic IndexPharmaceutical PreparationsCarbon based nanomaterialsGraphiteBiochemical engineeringSmall windowTalanta
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Immune Control in Hepatocellular Carcinoma Development and Progression: Role of Stromal Cells

2014

Immune control of hepatocellular carcinoma (HCC) is executed by effector immune cells, which efficiently eliminate malignant transformed cells. However, progression of HCC clearly documents failure of tumor immune control, which led to the concept of immune subversion by the tumor environment. Particularly tumor-associated stromal cells cooperate within an inflammatory network, which is responsible for immune privilege. The stromal cell composition matures during tumor growth and is derived from surrounding noncancerous tissue or from circulating cells recruited to the tumor site. Therefore, immunosuppressive stromal cells represent heterogeneous cell lineages, including myeloid cells, lymp…

Carcinoma HepatocellularStromal cellmedicine.medical_treatmentAdaptive ImmunityBiologyLymphocytes Tumor-InfiltratingImmune systemCancer immunotherapyImmune privilegeTumor MicroenvironmentLymph node stromal cellmedicineAnimalsHumansTumor microenvironmentHepatologyLiver Neoplasmsbiochemical phenomena metabolism and nutritionAcquired immune systemImmunity InnateB-1 cellImmunologyCytokinesbacteriaTumor EscapeImmunotherapyStromal CellsSignal TransductionSeminars in Liver Disease
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