Search results for "Biochemical"
showing 10 items of 870 documents
Gibberellic acid stimulates lipid metabolism in barley aleurone protoplasts
1991
Abstract Changes in the lipid composition of barley aleurone protoplasts following incubation in the presence or absence of gibberellic acid (GA) were studied and correlated with cell development. Analysis of neutral lipids in protoplast extracts and purified lipid bodies provide evidence for the strong effect of GA on the mobilization of storage lipids. In vivo protoplast staining with the membrane marker N -phenyl-l-naphthylamine (NPN) and phospholipid determinations of protoplast extracts show an increase in membrane lipids during protoplast incubation. GA stimulates phospholipid metabolism by increasing the synthesis of phosphatidyl ethanolamine and phosphatidyl choline, the major phosp…
Effects of Ga3 and Ca2+ on barley aleurone protoplasts: a freeze-fracture study
1988
Freeze-fracture electron microscopy was used to study changes in the endomembrane system of barley (Hordeum vulgare L. cv. Himalaya) aleurone protoplasts. Protoplasts were used for this study because their response to calcium and the plant hormone gibberellic acid (Ga3) can be monitored prior to rapid freezing of cells for electron microscopy. Protoplasts incubated in Ga3 plus Ca2+ secrete elevated levels of a-amylase relative to cells incubated in Ga3 or Ca2+ alone. The endoplasmic reticulum (ER) and Golgi apparatus of protoplasts incubated in Ga3 plus Ca2+ undergo changes that are well correlated with the synthesis and secretion of a-amylase. The ER, which appears as short, single sheets …
Subcellular fractionation of tissue culture cells.
2003
Cell fractionation techniques include some of the most important and widely used analytical tools in cell and molecular biology, and are essential for the development of cell-free assays that reconstitute complicated cellular processes. In addition to simple gradient systems, this unit discusses the immuno-purification of organelles, in particular endosomes. As antigens, purification can be achieved using endogenous or ectopically expressed proteins, provided that appropriate antibodies are available. Alternatively, tagged proteins can be used, when combined with anti-tag antibodies. Now that sequencing of the genomes of several organisms has been completed, biochemical strategies, and in p…
Alix regulates egress of hepatitis B virus naked capsid particles in an ESCRT-independent manner
2010
Hepatitis B virus (HBV) is an enveloped DNA virus that exploits the endosomal sorting complexes required for transport (ESCRT) pathway for budding. In addition to infectious particles, HBV-replicating cells release non-enveloped (nucleo)capsids, but their functional implication and pathways of release are unclear. Here, we focused on the molecular mechanisms and found that the sole expression of the HBV core protein is sufficient for capsid release. Unexpectedly, released capsids are devoid of a detectable membrane bilayer, implicating a non-vesicular exocytosis process. Unlike virions, naked capsid budding does not require the ESCRT machinery. Rather, we identified Alix, a multifunctional …
Long-term properties and end-of-life of polymers from renewable resources
2017
[EN] The long-term properties and end-of-life of polymers are not antagonist issues. They actually are inherently linked by the duality between durability and degradation. The control of the service-todisposal pathway at useful performance, along with low-impact disposal represents an added-value. Therefore, the routes of design, production, and discarding of bio-based polymers must be carefully strategized. In this sense, the combination of proper valorisation techniques, i.e. material, energetic and/ or biological at the most appropriate stage should be targeted. Thus, the consideration of the end-of-life of a material for a specific application, instead of the end-of-life of a material s…
Subtractive versus mass conserving metal shaping technologies: an environmental impact comparison
2015
The scientific studies in the domain of environmental sustainability of metal processing technologies predominantly focus on conventional material removal processes, as milling and turning. Despite some exceptions, many other non-machining technologies, such as metal forming processes, are still not well documented in terms of their energy and resource efficiency. Moreover, to properly evaluate the environmental impact of a given process, a standing-alone approach is no longer sufficient. In order to offer a valuable contribution in the domain of metal shaping sustainability, the present paper proposes a thorough methodology entailing to compare, from the environmental point of view, two tr…
An integrated model for biological and physical process simulation in membrane bioreactors (MBR).
2011
Abstract Mathematical modelling of membrane bioreactors (MBRs) for wastewater treatment has targeted either the biological processes (treatment quality target) as well as various aspects of system management. However, the high number of interactions among the involved physical–chemical processes, hampers a straightforward mathematical modelling. This circumstance is much more emphasized for submerged MBR systems where the membrane is immersed in an aeration tank. One of the main crucial points that prevents a comprehensive understanding is the interpretation of the fouling phenomenon and its connections with the biological processes. An overall mathematical model for MBR has not been comple…
Thermodynamics, transport phenomena, and electrochemistry of external field-assisted nonthermal food technologies
2017
Interest in the development and adoption of nonthermal technologies is burgeoning within the food and bioprocess industry, the associated research community, and among the consumers. This is evident from not only the success of some innovative nonthermal technologies at industrial scale, but also from the increasing number of publications dealing with these topics, a growing demand for foods processed by nonthermal technologies and use of natural ingredients. A notable feature of the nonthermal technologies such as cold plasma, electrohydrodynamic processing, pulsed electric fields, and ultrasound is the involvement of external fields, either electric or sound. Therefore, it merits to study…
comparing two start-up strategies for MBRs: experimental study and mathematical modelling
2012
Abstract The performance of a membrane bioreactor (MBR), and mechanisms of fouling formation, may differ due to the start-up. Therefore, the start-up can constitute an aspect that critically influences MBR performance during its lifespan. Indeed, the start-up can influence the mechanisms of membrane fouling, which is of paramount importance in an MBR. In order to gain insights on the effects of the start-up, both experimental and mathematical modelling studies were carried out on an MBR pilot plant. The MBR pilot plant constituted of a hollow fibre membrane module, in a submerged configuration, was fed by real wastewater. Two experimental periods were carried out, lasting 65 days each, char…
Global sensitivity analysis of a filtration model for submerged anaerobic membrane bioreactors (AnMBR)
2014
The results of a global sensitivity analysis of a filtration model for submerged anaerobic MBRs (AnMBRs) are assessed in this paper. This study aimed to (1) identify the less- (or non-) influential factors of the model in order to facilitate model calibration and (2) validate the modelling approach (i.e. to determine the need for each of the proposed factors to be included in the model). The sensitivity analysis was conducted using a revised version of the Morris screening method. The dynamic simulations were conducted using long-term data obtained from an AnMBR plant fitted with industrial-scale hollow-fibre membranes. Of the 14 factors in the model, six were identified as influential, i.e…