Search results for "Bioengineering"
showing 10 items of 1963 documents
Synthetic microbiology as a source of new enterprises and job creation: a Mediterranean perspective
2019
Peer reviewed
Dendritic cells, engineered to secrete a T-cell receptor mimic peptide, induce antigen-specific immunosuppression in vivo
2003
A T-cell receptor mimic peptide (TCRpep) consisting of an 8-amino-acid peptide, homologous to the transmembrane region of the T-cell receptor (TCR) alpha chain, blocks T-cell activation after systemic application. When dendritic cells (DCs) were transduced to secrete the TCRpep and injected into mice, evidence of immunosuppression was observed. In a CD8-driven allergy model, the injection of DCs transduced with the TCRpep reduced inflammation markedly and in a CD4+ T cell-dependent model of multiple sclerosis (experimental autoimmune encephalitis, EAE), injection of TCRpep-secreting DCs abrogated EAE symptoms and prolonged survival. These effects were antigen specific, because transduced DC…
Enrichment of Outgrowth Endothelial Cells in High and Low Colony-Forming Cultures from Peripheral Blood Progenitors
2009
An effective isolation protocol for outgrowth endothelial cells (OEC) resulting in higher cell numbers and a reduced expansion time would facilitate the therapeutical application. In this study a standard protocol based on the isolation of mononuclear cells from adult peripheral blood was modified by adding a passaging step 7 days after the isolation. OEC colonies gained by both protocols were evaluated after 28 days and resulted in different frequencies of OEC colonies depending on the donor and culture protocol. Accordingly, we defined two groups, namely, high colony-forming cultures (HCC) and low colony-forming cultures (LCC) for further analysis. LCC revealed no increase in OEC colonies…
Structural and functional role of Domain I for the insecticidal activity of the Vip3Aa protein from Bacillus thuringiensis
2022
12 p.-4 fig.-2 tab.
Dimensional analysis and stage-discharge relationship for weirs: a review
2017
Deducing the weir flow stage-discharge relationship is a classical hydraulic problem. In this regard Buckingham’s theorem of dimensional analysis can be used to find simple and accurate formulas to obtain the rating curves of different weir types. At first, in this review paper the rectangular weir that is a very common hydraulic structure is studied. It is indicated that the crest shape, approach channel width, obliquity (angle between the weir crest and the direction normal to the flow motion) and vertical inclination (pivot weir) are the key-parameters affecting the flow over the rectangular weirs. The flow over the triangular, labyrinth, parabolic, circular, elliptical, and W-weirs are …
Testing mechanical characteristics of chestnut stakes used in bed sills for stream restoration
2017
Using of wood elements for constructing bed sills in Mediterranean streams, where the banks are not protected by tree vegetation, needs an evaluation of biological and mechanical characteristics for evaluating both the wood durability and the effectiveness of the stream restoration project. Very few studies have dealt both with the decay of mechanical characteristics of wood elements employed for stream restoration works and with the changes over time of physical and chemical wood characters. In this paper, for a wood and stone bed sill located in a stream having no shaded banks, the changes of physical and chemical characters detected after 12, 18, 24, 30, 36, 42 and 48 months on chestnut …
Building green covering for a sustainable use of energy
2013
Nowadays the growth of the cities increased built and paved areas, energy use and heat generation. The phenomenon of urban warming, called urban heat island, influences negatively outdoor comfort conditions, pollutants concentration, energy demand for air conditioning, as well as increases environmental impact due to the demand of energy generation. A sustainable technology for improving the energy efficiency of buildings is the use of green roofs and walls in order to reduce the energy consumption for conditioning in summer and improve the thermal insulation in winter. The use of green roofs and walls can contribute to mitigate the phenomenon of heat island, the emissions of greenhouse gas…
A full body musculoskeletal model based on flexible multibody simulation approach utilised in bone strain analysis during human locomotion
2011
Load-induced strains applied to bone can stimulate its development and adaptation. In order to quantify the incident strains within the skeleton, in vivo implementation of strain gauges on the surfaces of bone is typically used. However, in vivo strain measurements require invasive methodology that is challenging and limited to certain regions of superficial bones only such as the anterior surface of the tibia. Based on our previous study [Al Nazer et al. (2008) J Biomech. 41:1036-1043], an alternative numerical approach to analyse in vivo strains based on the flexible multibody simulation approach was proposed. The purpose of this study was to extend the idea of using the flexible multibod…
Modelling and dynamic simulation of hybrid moving bed biofilm reactors: model concepts and application to a pilot plant.
2011
In the recent years, there has been an increasing interest in the development of hybrid reactors, especially in the up-grading of existing activated sludge plants that are no longer able to comply with concentration limits established by regulatory agencies. In such systems the biomass grows both as suspended flocs and as biofilm. In this way, it is possible to obtain a higher biomass concentration in the reactor, but without any significant increase of the load to the final clarifier. The paper presents the setting-up of a dynamic mathematical model aimed at quantitatively describing the biokinetic processes occurring in a hybrid moving bed biofilm reactor (HMBBR), and, more in general, in…
comparing two start-up strategies for MBRs: experimental study and mathematical modelling
2012
Abstract The performance of a membrane bioreactor (MBR), and mechanisms of fouling formation, may differ due to the start-up. Therefore, the start-up can constitute an aspect that critically influences MBR performance during its lifespan. Indeed, the start-up can influence the mechanisms of membrane fouling, which is of paramount importance in an MBR. In order to gain insights on the effects of the start-up, both experimental and mathematical modelling studies were carried out on an MBR pilot plant. The MBR pilot plant constituted of a hollow fibre membrane module, in a submerged configuration, was fed by real wastewater. Two experimental periods were carried out, lasting 65 days each, char…