Search results for "Biological activity"

showing 10 items of 465 documents

Synthesis and biological evaluation of 4-nitro-substituted 1, 3-diaryltriazenes as a novel class of potent antitumor agents

2011

Abstract We describe the synthesis and biological activity of a new class of 1,3-diaryltriazenes, namely 4-nitro-substituted 1,3-diaryltriazenes. Structure–activity relationship analysis reveals that 1,3-diaryltriazenes can be modified from inactive to highly cytotoxic compounds by the introduction of two nitro groups at the para positions of benzene rings and two additional electron-withdrawing groups (bromo, chloro, trifluoromethyl or fluoro substituents) at their ortho position. In order to increase the solubility of the modified compounds, we introduced various acyl groups to their triazene nitrogen. The results of LC-MS/MS analysis showed that N -acyltriazenes can be considered as prod…

StereochemistryNitro compoundAntineoplastic AgentsApoptosis1 ; 3-diaryltriazenes ; synthesis ; cytotoxicity ; ROS induction ; apoptosisChemical synthesisNitrophenolschemistry.chemical_compoundStructure-Activity RelationshipCell Line TumorDrug DiscoveryCytotoxic T cellHumansProdrugsTriazeneCell ProliferationPharmacologychemistry.chemical_classificationChemistryOrganic ChemistryBiological activityGeneral MedicineDNA NeoplasmProdrugEndoplasmic Reticulum StressIn vitroDrug Resistance NeoplasmNitroCisplatinTriazenesReactive Oxygen Species
researchProduct

Synthesis and preliminary antibacterial evaluation of Linezolid-like 1,2,4-oxadiazole derivatives

2012

In the present study the synthesis of new Linezolid-like molecules has been achieved by substitution of the oxazolidinone central heterocyclic moiety with a 1,2,4-oxadiazole ring. Two series of 1,2,4-oxadiazoles, bearing different side-chains and containing a varying number of fluorine atoms, were synthesized and preliminarily tested for biological activity against some Gram-positive and Gram-negative bacteria using Linezolid and Ceftriaxone as reference drugs

StereochemistryOxadiazoleMicrobial Sensitivity TestsAntimicrobial activityRing (chemistry)Oxazolidinone 124-Oxadiazole Linezolid Antimicrobial activity Fluorinated heterocyclesStructure-Activity Relationshipchemistry.chemical_compoundFluorinated heterocyclesAcetamidesDrug DiscoveryMoleculeMoiety124-OxadiazoleOxazolidinonesPharmacologyBacteriaMolecular StructureCeftriaxoneOrganic ChemistryLinezolidBiological activitySettore CHIM/06 - Chimica OrganicaGeneral MedicineSettore CHIM/08 - Chimica FarmaceuticaOxazolidinoneAnti-Bacterial AgentschemistryMED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICALinezolid
researchProduct

Phytochemical Profile and Apoptotic Activity of Onopordum cynarocephalum.

2012

A phytochemical investigation of acetone and chloroform extracts of the aerial parts of Onopordum cynarocephalum Boiss. et Blanche was carried out. It led to the isolation of two new sesquiterpenes, the elemane aldehyde (2) and the eudesmane (11), together with 15 known compounds: two lignans (1 and 15) and 13 sesquiterpenes (3–10, 12–14, 16, 17). The structures were elucidated by spectroscopic analyses, especially 1D and 2D NMR spectra. The anti-growth effect against three human melanoma cell lines, M14, A375, and A2058, of the different extracts and compounds of O. cynarocephalum was also investigated. Among them, the chloroform extract exhibited the strongest biological activity, while t…

StereochemistryPharmaceutical ScienceApoptosisDNA FragmentationLignansAnalytical Chemistrychemistry.chemical_compoundInhibitory Concentration 50cytotoxic activity Onopordum cynarocephalum Boiss. et BlancheCell Line TumorDrug DiscoveryHumansSesquiterpenes EudesmaneHSP70 Heat-Shock ProteinsFuransArctigeninCell ProliferationPharmacologyLignanChloroformPlants MedicinalbiologyDose-Response Relationship DrugMolecular StructureCaspase 3Plant ExtractsOrganic ChemistryOnopordumPTEN PhosphohydrolaseBiological activityPlant Components AerialAntineoplastic Agents PhytogenicEnzyme assayMonocyclic SesquiterpenesComplementary and alternative medicinePhytochemicalchemistryApoptosisbiology.proteinMolecular MedicineDNA fragmentationSesquiterpenes
researchProduct

Tetrandrine and Isotetrandrine, Two Bisbenzyltetrahydroisoquinoline Alkaloids from Menispermaceae, with Rat Uterine Smooth Muscle Relaxant Activity

1992

Abstract The effects of two bisbenzyltetrahydroisoquinoline alkaloids, 1S, 1′S tetrandrine and its isomer 1R, 1′S isotetrandrine, were investigated in rat isolated uterus in order to identify the mechanism of relaxant action and to study the influence of the absolute configuration on the activity of these alkaloids. Both inhibited the uterine contraction induced by high K+, acetylcholine and oxytocin. In Ca2+-free medium, isotetrandrine relaxed the sustained contraction induced by oxytocin but tetrandrine did not. The relaxant effects of the alkaloids may be due to blockade of calcium influx through specific channels. Tetrandrine and isotetrandrine modify the calcium channel in a nonreversi…

StereochemistryPharmaceutical ScienceIn Vitro TechniquesPharmacologyOxytocinBenzylisoquinolinesUterine contractionUterine Contractionchemistry.chemical_compoundAlkaloidsmedicineAnimalsPharmacologyDose-Response Relationship Drugbusiness.industryAlkaloidCalcium channelAnti-Inflammatory Agents Non-SteroidalUterusMuscle SmoothRats Inbred StrainsStereoisomerismBiological activityAcetylcholineRatsTetrandrineOxytocinchemistryDepression ChemicalFemalemedicine.symptombusinessAcetylcholinemedicine.drugMuscle contractionJournal of Pharmacy and Pharmacology
researchProduct

α-Adrenoceptor Interaction of Tetrandrine and Isotetrandrine in the Rat: Functional and Binding Assays

1998

Abstract The action of 1S,1′S-tetrandrine, a bisbenzyltetrahydroisoquinoline alkaloid, on α1-adrenoceptors has been compared with that of its isomer 1R,1′S-isotetrandrine. The work includes binding assays to analyse the affinity of these products for the [3H]prazosin binding site of rat cerebral cortical membranes and functional studies on rat isolated aorta to examine the effects of both alkaloids on intracellular calcium processes related or not to α-adrenoceptor activation. A radioligand receptor-binding study showed that both compounds interacted with the α1-adrenoceptors displacing [3H]prazosin from the specific binding site. The Ki values (inhibition constants) were 0.69±0.12 and 1.6±…

StereochemistryPharmaceutical Sciencechemistry.chemical_elementAorta ThoracicIn Vitro TechniquesBiologyPharmacologyCalciumTritiumBenzylisoquinolinesBinding CompetitiveMuscle Smooth VascularCalcium in biologyNorepinephrinechemistry.chemical_compoundAlkaloidsPrazosinmedicineExtracellularAnimalsDrug InteractionsRats WistarCerebral CortexPharmacologyBinding SitesMolecular StructureAlkaloidBiological activityPrazosinReceptors Adrenergic alphaCalcium Channel BlockersRatsTetrandrinechemistryCalciumFemaleIntracellularMuscle ContractionProtein Bindingmedicine.drugJournal of Pharmacy and Pharmacology
researchProduct

Cytotoxic Isomalabaricane Triterpenes from the Marine Sponge Rhabdastrella globostellata

2006

Fourteen isomalabaricane triterpenes were isolated from the marine sponge Rhabdastrella globostellata. In addition to the known compounds globostellatic acids A (1) and D (4) and stelliferin riboside (13), 11 of the compounds were new natural products, which included globostelletin (3), eight new globostellatic acid congeners, F to M (2, 5-11), and two new stelliferin ribosides (12 and 14). The isolated compounds were tested against three different cancer cell lines, L5178Y (mouse lymphoma), HeLa (human cervix carcinoma), and PC-12 (rat pheochromocytoma). The isomalabaricane derivatives were found to be selectively active toward the mouse lymphoma cell line L5178Y. The structures were deter…

StereochemistryRhabdastrella globostellataPharmaceutical ScienceAntineoplastic AgentsPharmacognosyPC12 CellsAnalytical ChemistryHeLaTerpeneMiceTriterpeneDrug DiscoveryTumor Cells CulturedAnimalsHumansPharmacologychemistry.chemical_classificationMolecular StructurebiologyOrganic ChemistryBiological activitybiology.organism_classificationTriterpenesTerpenoidPoriferaRatsSpongeComplementary and alternative medicinechemistryIndonesiaMolecular MedicineDrug Screening Assays AntitumorHeLa CellsJournal of Natural Products
researchProduct

Methomyl analogues with increased biological activity towards F7T maize mitochondria

1987

Abstract Methomyl analogues were synthesized by substituting alkyl moieties (C 2 -C 21 ) in the place of the carbamic methyl. They were assayed on mitochondria isolated from male sterile (F 7 T) and male fertile (F 7 N) maize. They had no action on F 7 N mitochondria. The heptadecyl (C 17 ) and heneicosanyl (C 21 ) derivatives had no conspicuous effect on F 7 T mitochondria. By contrast, the ethyl, propyl, butyl, nonyl, tridecyl (C 13 ) and pentadecyl (C 15 ) derivatives had the same type of activity as Methomyl on F 7 T mitochondria, namely stimulation of NADH oxidation and inhibition of malate oxidation. Moreover, the concentration at which they were maximally effective decreased from 10 …

StereochemistryStimulationMethomylPlant ScienceHorticultureBiologyMitochondrionmedicine.disease_causeBiochemistry03 medical and health scienceschemistry.chemical_compoundmedicineHelminthosporium maydisMolecular Biology[SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacyAlkylComputingMilieux_MISCELLANEOUS030304 developmental biologychemistry.chemical_classification0303 health sciencesToxinBiological activity04 agricultural and veterinary sciencesGeneral MedicineFungi imperfectibiology.organism_classification[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacyGRAMINEchemistryBiochemistry040103 agronomy & agriculture0401 agriculture forestry and fisheries
researchProduct

Synthesis, structural characterisation and biological studies of new mononuclear platinum(II) complexes with sterically hindered heterocyclic ligands

2011

Abstract Three novel cisplatin analogues were synthesized, designed according to an approach which violates the “classical” structure–activity relationship, by replacing the diamine ligands with a planar N donor heterocycle giving a sterically hindered complex. Moreover, the sterical hindrance of antitumor drug candidates potentially makes them less susceptible to deactivation by sulphur-containing proteins and helping to overcome resistance mechanisms. The resulting mononuclear complexes of sterically hindered polidentate heterocyclic N ligands [PtCl(bbp)]Cl ( 1 ) [bbp = 2,6-bis(2-benzimidazolyl)pyridine], [PtCl 2 (dptdn)](H 2 O) ( 2 ) [dptdn = sodium 5,6-diphenyl-3-(2′-pyridyl)-1,2,4-tria…

Steric effectsCisplatinStereochemistryCytotoxicitychemistry.chemical_elementBiological activityPolydentate nitrogen ligandInorganic Chemistrychemistry.chemical_compoundchemistryPolynuclear platinum complexeSettore CHIM/03 - Chimica Generale E InorganicaDiaminePyridineMaterials ChemistrymedicinePhysical and Theoretical ChemistryBifunctionalCytotoxicityPlatinummedicine.drugPolydentate nitrogen ligands; Polynuclear platinum complexes; Cytotoxicity
researchProduct

Effects of Hypericum Extract on the Expression of Serotonin Receptors

1994

The influence of hypericum extract LI 160 on the expression of serotonin receptors was investigated using a neuroblastoma cell line to establish a model for the regulation of neurotransmitters by immunologically active compounds such as cytokines. The cells were incubated with hypericum extract LI 160 in kinetic form for 2, 4, 6, 8, and 10 hours, then washed. The serotonin receptor expression analysis was compared to that of a placebo control solution. The neuroblastoma cells showed a clearly reduced expression of the serotonin receptors under treatment with hypericum extract. First stimulation experiments with interleukin-1 (IL-1) and hypericum extract suggest that a further reduction of …

StimulationPharmacologyPharmacognosyNeuroblastoma cellNeuroblastoma03 medical and health sciences0302 clinical medicineTumor Cells CulturedAnimalsHypericum extractPerylene5-HT receptorPlants Medicinal030214 geriatricsPlant ExtractsChemistryHypericum perforatumBiological activityAntidepressive AgentsIn vitroRatsPsychiatry and Mental healthXanthenesReceptors SerotoninQuercetinSerotonin AntagonistsNeurology (clinical)Geriatrics and GerontologyHypericum030217 neurology & neurosurgeryInterleukin-1Journal of Geriatric Psychiatry and Neurology
researchProduct

Packing meterials with surface barriers, controlled distribution and topography of ligands for sample clean-up and analysis of biologically active so…

1991

Various types of, so-called, restricted access packings have been developed for sample clean-up and analysis of biologically active solutes by means of HPLC. The unique feature of these packings is that they prevent the access of matrix components such as protein whilst selectively retaining the drug components and their metabolites. This results from the fact that the particles of the packings possess a surface barrier for large solutes at the external or internal surface area and exhibit a controlled gradient in the chemical surface composition. They are grouped, according to their particle structures, into internal-surface reversed phases (ISRP), shielded hydrophobic phases (SHP), semi-p…

Surface (mathematics)Surface barrierChromatographyChemistryOrganic ChemistryClinical BiochemistryRestricted accessBiological activityBiochemistryHigh-performance liquid chromatographyAnalytical ChemistryClean-upMatrix (chemical analysis)ParticleChromatographia
researchProduct