Search results for "Bios"

Different strategies to achieve Pb-tolerance by the two Trebouxia algae coexisting in the lichen Ramalina farinacea.

2012

Lichen thalli are permeable to airborne substances, including heavy metals, which are harmful to cell metabolism. Ramalina farinacea shows a moderate tolerance to Pb. This lichen comprises two Trebouxia phycobionts, provisionally referred to as TR1 and TR9, with distinct physiological responses to acute oxidative stress. Thus, there is a more severe decay in photosynthesis and photosynthetic pigments in TR1 than in TR9. Similarly, under oxidative stress, antioxidant enzymes and HSP70 protein decrease in TR1 but increase in TR9. Since Pb toxicity is associated with increased ROS formation, we hypothesized greater Pb tolerance in this phycobiont. Accordingly, the aim of the present study was …

The genetic structure of the cosmopolitan three-partner lichenRamalina farinaceaevidences the concerted diversification of symbionts

2012

The epiphytic lichen Ramalina farinacea is distributed throughout the northern hemisphere in which the same two algal Trebouxia species (provisionally named TR1 and TR9) coexist in every thallus. Ramalina farinacea symbionts were characterized based on the two fungal nuclear loci (nrITS and rpb2 ) along with the primary and secondary structures of nrITS from each Trebouxia species in the Iberian Peninsula and Canary Islands. The results indicated a noticeable genetic differentiation between mycobionts from these two geographic areas and also suggested concerted changes in the three partners of a lichen symbiosis toward two clearly distinguishable ‘holobiont’ lineages. Modeling of ITS2 RNA s…

Differences in the cell walls and extracellular polymers of the two Trebouxia microalgae coexisting in the lichen Ramalina farinacea are consistent w…

2015

Trebouxia TR1 and T. TR9 are the two microalgae that coexist within Ramalina farinacea thalli. In the presence of Pb, TR9 formed extracellular aggregates, while TR1 showed a lower wall lead retention capability. Herein, we studied the cell walls and extracellular polymers (EPS) of TR1 and TR9, and their possible implication in the different Pb retention capacity of these microalgae. The proportion of cell walls on the overall cell biomass was 2.6 times higher in TR9 than in TR1. Glycosyl linkage analysis indicated the presence of hot-water soluble β-galactofuranan(s) in both cell walls, distinct from that previously described in Asterochloris, with increased rhamnose content in TR9 and a hi…

Chloroplast morphology and pyrenoid ultrastructural analyses reappraise the diversity of the lichen phycobiont genus Trebouxia (Chlorophyta)

2022

Abstract Trebouxiophyceae is a wide class of green algae comprising coccoid and elliptic unicells, filaments, blades and colony-forming species that occur in diverse terrestrial and aquatic environments. Within this class, the genus Trebouxia Puymaly is among the most widespread lichen phycobionts worldwide. However, the 29 formally described species based on the combination of morphological traits and genetic diversity woefully underrepresented the overall species-level diversity recognized in the genus. In Trebouxia, reliable differentiation and characterization of the species-level lineages can be achieved by studying the diversity of key diagnostic features of pyrenoid ultrastructure an…

Bacterial Ectosymbionts which Confer Motility: Mixotricha paradoxa from the Intestine of the Australian Termite Mastotermes darwiniensis

2005

Suppression of intestinal microbiota-dependent production of pro-atherogenic trimethylamine N-oxide by shifting L-carnitine microbial degradation.

2014

Abstract Aims Trimethylamine-N-oxide (TMAO) is produced in host liver from trimethylamine (TMA). TMAO and TMA share common dietary quaternary amine precursors, carnitine and choline, which are metabolized by the intestinal microbiota. TMAO recently has been linked to the pathogenesis of atherosclerosis and severity of cardiovascular diseases. We examined the effects of anti-atherosclerotic compound meldonium, an aza-analogue of carnitine bioprecursor gamma-butyrobetaine (GBB), on the availability of TMA and TMAO. Main methods Wistar rats received L-carnitine, GBB or choline alone or in combination with meldonium. Plasma, urine and rat small intestine perfusate samples were assayed for L-car…

The (2-phenyl-2-trimethylsilyl)ethyl-(PTMSEL)-linker in the synthesis of glycopeptide partial structures of complex cell surface glycoproteins.

2003

The (2-phenyl-2-trimethylsilyl)ethyl-(PTMSEL) linker represents a novel fluoride-sensitive anchor for the solid-phase synthesis of protected peptides and glycopeptides. Its cleavage is achieved under almost neutral conditions using tetrabutylammonium fluoride trihydrate in dichloromethane thus allowing the construction of complex molecules sensitive to basic and acidic media commonly required for the cleavage of standard linker systems. The advantages of the PTMSEL linker are demonstrated in the synthesis of glycopeptides from the liver intestine (LI)-cadherin and the mucin MUC1, bearing carbohydrate moieties such as N-linked chitobiose or O-linked sialyl-T(N)-residues. The synthesis of the…

Biocénoses à triopsidés(Crustacea, Branchiopoda) du Permien du bassin de Lodève (France)

1997

Resume Nous decrivons les triopsides (Crustacea, Branchiopoda) qui abondent dans les facies “saxoniens” de la Formation du Salagou du bassin de Lodeve date “Thuringien” ou “Saxonien” selon les auteurs. A partir de milliers de debris de carapaces, d'axes thoraco-abdominaux et plus rarement a partir d'animaux entiers, nous avons identifie et decrit deux especes nouvelles: Triops cancriformis permiensis nov. ssp. et Lepidurus occitaniacus nov. sp., morphologiquement proches d' especes actuelles. En se basant sur les preferences ecologiques des triopsides actuels et les caracteristiques sedimentologiques de leur milieu de vie au Permien, nous avons confirme l'existence d'etendues d'eau, peu pro…

Dolomitization as an eventualdetermining factor in the colour alteration index (CAI)

1993

Abstract The Colour Alteration Index of conodonts (CAI) is indicative of the degree of alteration of organic material that exists in the interior of the conodonts. This CAI is used ordinarily in the determination of paleotemperatures. More frequent findings of abnormally high CAI values, equivalent to paleotemperatures that are not compatible with temperatures obtained by other more trustworthy methods, has taken us to use values not superior to 6 for the establishment of paleotemperatures. The CAI values measured in specimens proceding from dolostones in the Iberian Range (Spain) show in this way a certain degree of incompatibility with geological data. These anomalous values are the conse…

3D-Structure and function of strictosidine synthase--the key enzyme of monoterpenoid indole alkaloid biosynthesis.

2008

Strictosidine synthase (STR; EC 4.3.3.2) plays a key role in the biosynthesis of monoterpenoid indole alkaloids by catalyzing the Pictet-Spengler reaction between tryptamine and secologanin, leading exclusively to 3alpha-(S)-strictosidine. The structure of the native enzyme from the Indian medicinal plant Rauvolfia serpentina represents the first example of a six-bladed four-stranded beta-propeller fold from the plant kingdom. Moreover, the architecture of the enzyme-substrate and enzyme-product complexes reveals deep insight into the active centre and mechanism of the synthase highlighting the importance of Glu309 as the catalytic residue. The present review describes the 3D-structure and …