Search results for "Bios"
showing 10 items of 2557 documents
Molecular Approaches Fighting Nonsense
2021
Nonsense mutations are the result of single nucleotide substitutions in the DNA that change a sense codon (coding for an amino acid) to a nonsense or premature termination codon (PTC) within the coding region of the mRNA [...]
Sterigmatocystin: Occurrence, toxicity and molecular mechanisms of action – A review
2020
The mycotoxin sterigmatocystin (STE) is produced mainly by Aspergillus fungi. It has been reported to occur in grains and grain-based products, cheese, coffee, spices and beer. The STE is a known biogenic precursor of aflatoxin B1, sharing with it several structural and biological similarities. The STE has been shown to be hepatotoxic and nephrotoxic in animals and it has been classified as possible human carcinogen (group 2B) by IARC. The STE has been reported to cause a marked decrease in cell proliferation in different mammalian cells. Data available on literature suggest that the cellular mechanisms underlying STE-induced toxicity include the induction of oxidative stress, mitochondrial…
Expression, purification, crystallization and preliminary X-ray analysis of perakine reductase, a new member of the aldo-keto reductase enzyme superf…
2006
Perakine reductase (PR) is a novel member of the aldo-keto reductase enzyme superfamily from higher plants. PR from the plant Rauvolfia serpentina is involved in the biosynthesis of monoterpenoid indole alkaloids by performing NADPH-dependent reduction of perakine, yielding raucaffrinoline. However, PR can also reduce cinnamic aldehyde and some of its derivatives. After heterologous expression of a triple mutant of PR in Escherichia coli, crystals of the purified and methylated enzyme were obtained by the hanging-drop vapour-diffusion technique at 293 K with 100 mM sodium citrate pH 5.6 and 27% PEG 4000 as precipitant. Crystals belong to space group C222(1) and diffract to 2.0 A, with unit-…
Idiosyncratic responses to simulated herbivory by root fungal symbionts in a subarctic meadow
2021
Plant-associated fungi have elementary roles in ecosystem productivity. There is little information on the interactions between arbuscular mycorrhizal (AM) fungal symbiosis, fine endophytic (FE) and dark septate endophytic (DSE) fungi, and their host plants in cold climate systems. In particular, the environmental filters potentially driving the relative abundance of these root symbionts remain unknown. We investigated the interlinkage of plant and belowground fungal responses to simulated herbivory (clipping, fertilization, and trampling) in a subarctic meadow system. AM and FE frequency in the two target plant roots, Potentilla crantzii and Saussurea alpina, was unaffected by simulated he…
Heritable Epichloë symbiosis shapes fungal but not bacterial communities of plant leaves
2019
Keystone microbial species have driven eco-evolutionary processes since the origin of life. However, due to our inability to detect the majority of microbiota, members of diverse microbial communities of fungi, bacteria and viruses have largely been ignored as keystone species in past literature. Here we tested whether heritable Epichloë species of pooidae grasses modulate microbiota of their shared host plant. peerReviewed
Human Enterovirus Group B Viruses Rely on Vimentin Dynamics for Efficient Processing of Viral Nonstructural Proteins.
2019
A virus needs the host cell in order to replicate and produce new progeny viruses. For this, the virus takes over the host cell and modifies it to become a factory for viral proteins. Irrespective of the specific virus family, these proteins can be divided into structural and nonstructural proteins. Structural proteins are the building blocks for the new progeny virions, whereas the nonstructural proteins orchestrate the takeover of the host cell and its functions. Here, we have shown a mechanism that viruses exploit in order to regulate the host cell. We show that viral protein synthesis induces vimentin cages, which promote production of specific viral proteins that eventually control apo…
SMOS-IC: An Alternative SMOS Soil Moisture and Vegetation Optical Depth Product
2017
© 2017 by the authors. The main goal of the Soil Moisture and Ocean Salinity (SMOS) mission over land surfaces is the production of global maps of soil moisture (SM) and vegetation optical depth (τ) based on multi-angular brightness temperature (TB) measurements at L-band. The operational SMOS Level 2 and Level 3 soil moisture algorithms account for different surface effects, such as vegetation opacity and soil roughness at 4 km resolution, in order to produce global retrievals of SM and τ. In this study, we present an alternative SMOS product that was developed by INRA (Institut National de la Recherche Agronomique) and CESBIO (Centre d'Etudes Spatiales de la BIOsphère). One of the main go…
Cambios dimensionales en la unión dentogingival desde la erupción del diente hasta la edad adulta
2018
Introducción y objetivos. Los tejidos que rodean el diente y conforman el periodonto coronal sufren una serie de cambios estructurales y anatómicos importantes a lo largo de su desarrollo, desde que los dientes empiezan su erupción en la cavidad bucal hasta que alcanzan la oclusión con su antagonista. De estos cambios, poco sabemos, puesto que hay escasa literatura al respecto. Por tanto, en este estudio se ha pretendido aportar mas información sobre como se organizan los tejidos periodontales, especialmente a nivel de la unión dentogingival, durante el desarrollo de la erupción del diente hasta que el individuo alcanza la edad adulta; además, evaluar si alguna variación en el desa…
Estudio in vitro del láser de Nd:Yag sobre la superficie del esmalte: efectos estructurales, cambios morfológicos y térmicos
2016
En esta tesis doctoral se realizó un estudio in vitro restrospectivo mediante la aplicación del láser de Nd:YAG sobre la superficie del esmalte dental humano, con una variedad de parámetros concreta, valorarando la capacidad de producir cambios favorables estructurales y morfológicos sin fisuras ni grietas, sin producir alteración térmica en la pulpa ni otros efectos indeseados, con la finalidad de impermeabilizar dicha superficie y, como consecuencia, impidiendo el cambio iónico entre estas zonas y la solución desmineralizante, lo que evita la penetración del ácido orgánico en las capas internas del esmalte. Hipótesis y Objetivos: Se plantean como objetivos fundamentales los siguientes: 1.…
La fauna del pleistoceno inferior de la Sierra de Quibas (Abanilla, Murcia)
1999
El yacimiento kárstico cuaternario de la Sierra de Quibas (Abanilla, Murcia) ha proporcionado una amplia lista faunística constituida por 53 especies repartidas entre gasterópodos, miriápodos, anfibios, reptiles, aves y mamíferos. En el conjunto de los gasterópodos destaca Palaeoglandina, un género que se extingue en el resto de Europa durante el Plioceno y que se mantiene como relicto en el Pleistoceno de la Península Ibérica. Dos de las especies de aves determinadas, Gypaetus barbatus y Gerontieus eremita, y una de las serpientes, Elaphe cf. E. sealaris, apenas se hallan representadas en el registro fósil. Hay además varias especies de aves cuya aparición en Quibas supone la primera cita …