Search results for "Biosilicification"

showing 2 items of 2 documents

Biosilicification of loricate choanoflagellate: organic composition of the nanotubular siliceous costal strips of Stephanoeca diplocostata.

2010

SUMMARY Loricate choanoflagellates (unicellular, eukaryotic flagellates; phylum Choanozoa) synthesize a basket-like siliceous lorica reinforced by costal strips (diameter of approximately 100 nm and length of 3 μm). In the present study, the composition of these siliceous costal strips is described, using Stephanoeca diplocostata as a model. Analyses by energy-dispersive X-ray spectroscopy (EDX), coupled with transmission electron microscopy (TEM), indicate that the costal strips comprise inorganic and organic components. The organic, proteinaceous scaffold contained one major polypeptide of mass 14 kDa that reacted with wheat germ agglutinin. Polyclonal antibodies were raised that allowed …

PhysiologyLoricate choanoflagellateBiosilicificationNanotechnologyStructural analysisOrganic compositionAquatic ScienceAlkaliesStephanoeca diplocostataHydrofluoric AcidBiosilicification; Loricate choanoflagellate; Organic composition; Structural analysisAnimalsChemical PrecipitationOrganic ChemicalsChoanoflagellateMolecular BiologyEcology Evolution Behavior and SystematicsChoanoflagellatabiologyChemistryAnimal StructuresProteinsSpectrometry X-Ray Emissionbiology.organism_classificationElementsSilicon DioxideImmunohistochemistryWheat germ agglutininNanostructuresSolutionsElectrophoresisTransmission electron microscopyInsect ScienceBiophysicsAnimal Science and ZoologyComposition (visual arts)Electrophoresis Polyacrylamide GelLorica (biology)ChoanozoaThe Journal of experimental biology
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A Biocatalytic Nanomaterial for the Label-Free Detection of Virus-Like Particles

2017

International audience; The design of nanomaterials that are capable of specific and sensitive biomolecular recognition is an on-going challenge in the chemical and biochemical sciences. A number of sophisticated artificial systems have been designed to specifically recognize a variety of targets. However, methods based on natural biomolecular detection systems using antibodies are often superior. Besides greater affinity and selectivity, antibodies can be easily coupled to enzymatic systems that act as signal amplifiers, thus permitting impressively low detection limits. The possibility to translate this concept to artificial recognition systems remains limited due to design incompatibilit…

Silica mineralizationBiosilicificationNanoparticleNanotechnology02 engineering and technology010402 general chemistry01 natural sciencesBiochemistryNanomaterialsMolecular ImprintingSilica nanoparticlesMolecular recognitionArtificial systems[SDV.IDA]Life Sciences [q-bio]/Food engineeringOrganosilicaMolecular BiologyLabel freeDetection limitChemistryOrganic ChemistryVirion[ SDV.IDA ] Life Sciences [q-bio]/Food engineering021001 nanoscience & nanotechnologyNanostructures0104 chemical sciencesVirusesBiocatalysisMolecular MedicineNanoparticlesMechanismMolecular recognition0210 nano-technologyMolecular imprinting
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