Search results for "Biotechnology"

Gamma Irradiation and Fermentation

2016

This chapter discusses the applications of gamma irradiation technology for food safety, its nutritional implications, and its involvement in fermentation processes. Gamma irradiation has become an alternative technology for food sterilization due to its nonthermal character, thus replacing the conventional heating processes. Several driving forces are propelling the need of γ-irradiation forward for food applications. Besides food preservation, γ-irradiation is taking place for novel applications, especially involving the enhancement of food fermentation processes, by directly irradiating the medium, or generating performant genetically modified strains.

Foreword

2007

Interaction of Oily Water with Floating Porous Ceramic and Immobilized Microorganisms

2018

Oily water was treated with porous ceramic granules and immobilized microorganisms. Floating granules with bulk density of 0.63-0.66 g cm-3were used. The sorption of motor oil was investigated for dry and wetted granules. Respiration experiments showed that microorganisms immobilized on floating ceramic carrier and treated with oily water were influenced by agitation of liquid. The treatment of oily water with low salinity (1-9 g L-1) showed that oil removal efficiency decreased after increasing water salinity. Likewise, oil removal efficiency decreased from 12 to 9% per gram of ceramic carrier after increasing the spill of oil from 9 to 35 g per square meter. Porous granules with immobiliz…

Supercritical fluid extraction of algae enhances levels of biologically active compounds promoting plant growth

2016

The aim of this research was to screen plant growth biostimulant properties of supercritical CO2 macroalgal extracts. To this end secondary metabolites were isolated from the biomass of marine macroalgae from the Baltic Sea (species of Polysiphonia, Ulva and Cladophora). Chemical characteristics of the algal extracts were determined by inductively coupled plasma atomic emission spectroscopy for inorganic constituents and high-performance liquid chromatography and spectrophotometry for organic constituents. Inorganic (macro- and microelements) and organic (plant hormones: auxins and cytokinins; polyphenols) compounds were detected in the extract. Algal extracts were tested primarily on garde…

Characterization of Purified Green Bell Pepper Hydroperoxide Lyase Expressed by Yarrowia Lipolytica: Radicals Detection during Catalysis

2007

International audience; The optimization of the expression of recombinant 6-His-tagged HPO lyase in Yarrowia lipolytica is described: 1800U/L of culture was detected at 24h of culture on a medium containing olive oil as the sole carbon source. The enzyme was purified by IMAC and showed an optimal pH at 5.5, an optimal temperature at 20^\circC and a Km value of 9μM with 13-HPOD substrate. The participation of radicals during the catalysis of purified bell pepper fruit hydroperoxide lyase has been observed by electron paramagnetic resonance spectroscopy and the yet unidentified radical species might be an alkyl or alkoxyl radical linked to the enzyme.

Fruit and juice characteristics of 30 accessions of two cactus pear species (Opuntia ficus indica and Opuntia megacantha) from different regions of M…

2016

Abstract The fruit and fruit juice of 30 accessions of cactus pear (23 accessions of Opuntia ficus indica and 7 accessions of Opuntia megacantha ) grown in different regions of Morocco were analyzed. The morphological characteristics of the fruit and the pH, titratable acidity, contents of soluble solids, total carotenoids, reducing sugars, and vitamin C in the fruit juice were analyzed. Fruit length, fresh fruit weight, fresh skin weight, fresh skin to fruit ratio, dry skin weight, and water content in the skin varied significantly within and between the two species. There were significant differences among accessions in most of the fruit juice characteristics. The ranges of measured value…

Changes in the free amino acids and the biogenic amine contents during lactic acid fermentation of different lupin species

2016

Summary The aim of the work was to evaluate the profile of free amino acids (FAA) as the possible precursors of biogenic amines (BAs) in different varieties and hybrid lines of Lupinus luteus and Lupinus angustifolius and its changes during fermentation. Lupins have high amounts of FAA, and therefore, significant contents of BAs by the action of bacterial amino acid decarboxylases can be formed. In view of this, the effect of three Pediococcus pentosaceus strains KTU05-8, KTU05-9 and KTU05-10 on BA formation during lupin fermentation was studied. The formation of BAs was monitored during 48-h fermentation at solid-state conditions and compared with that in samples after submerged fermentati…

Evaluating the impact of vegetal and microalgae protein sources on proximate composition, amino acid profile, and physicochemical properties of ferme…

2018

Influence of pulsed electric field processing on the quality of fruit juice beverages sweetened with Stevia rebaudiana

2017

Abstract A fruit juice-stevia beverage was processed using pulsed electric fields (PEF), a non-thermal preservation technology, with the purpose of investigating the feasibility of PEF for bioactive compounds and steviol glycosides enhancement and its impact on physicochemical properties. Variable ranges of response surface methodology were 20–40 kV/cm (electric field strength), 100–360 μs (treatment time) and 0–2.5% (w/v) stevia. After PEF, ascorbic acid was retained by more than 74%. Some of the analyzed PEF treatments resulted in an enhancement of total anthocyanins and carotenoids. The best results for rebaudioside A/stevioside ratio were obtained when PEF was applied at 30 kV/cm for 23…

Effect of Enzyme Amounts Used in Gastrointestinal Digestion Upon Solubility and Caco-2 Cell Uptake Assays of Minerals from Infant Formulas

2005

The effect of enzyme amounts used in gastrointestinal in vitro digestion upon the solubility and Caco-2 cell uptake of calcium, iron and zinc from infant formulas (IFs) was studied. Different amounts of enzymes (g enzyme/g IF), pepsin (0.002 and 0.048), pancreatin (0.0005, 0.002 and 0.01) and bile extract (0.003, 0.125 and 0.0625) were assayed. Mineral soluble contents and mineral uptakes by Caco-2 cells were affected by the enzyme amounts used in digestion. Although the highest mineral solubility (Ca 98.6 vs 46.2%; Fe 98.1 vs 83.9%; Zn 98.4 vs 83%) was obtained when the lowest enzyme (pepsin 0.002 vs 0.048; pancreatin 0.0005 vs 0.01g/g IF) and bile extract (0.003 vs 0.0625g/g IF) amounts …