Search results for "Bleach"
showing 10 items of 115 documents
Evaluation of the effect of different enamel surface treatments and waiting times on the staining prevention after bleaching
2017
Background Bleached dental enamel can be more susceptible to staining than the enamel that has never been bleached, especially right after tooth bleaching. The aim of this study is to evaluate the effect of surface treatments and waiting time prior to contact with dye on bleached enamel staining susceptibility. Material and Methods One hundred teeth were bleached with 35% hydrogen peroxide (Whiteness HP, FGM) and randomly assigned to G1 artificial saliva, G2 2% sodium fluoride (Flugel, Nova DFL), G3 casein phosphopeptide-amorphous calcium phosphate fluoride paste (CPP-ACPF, MI Paste Plus, GC America), G4 rinse for color maintenance after bleaching (Keep White Rinse, DMC) and G5 polishing wi…
Different Behaviour of Plasma Antioxidant Status after Red Wine Consumption in Subjects of the Same Sex: A Preliminary Report
2012
<p>Several studies report that the plasma antioxidant capacity (PAC) increases after a single ingestion of red wine. However, data on the different behaviour of PAC after food intake in subjects of the same sex are still lacking. On the basis of these observations some investigations were undertaken in order to evaluate the effects of red wine intake on PAC in a homogeneous groups of healthy female volunteers (n=18). Additionally, the possible correlation between increase of PAC values and increase in uric acid levels was also assessed. PAC and uric acid concentrations were determined before wine intake and 50, 120 and 240 minutes thereafter. The results obtained following these studi…
Polar Localization of a Tripartite Complex of the Two-Component System DcuS/DcuR and the Transporter DctA in Escherichia coli Depends on the Sensor K…
2014
The C4-dicarboxylate responsive sensor kinase DcuS of the DcuS/DcuR two-component system of E. coli is membrane-bound and reveals a polar localization. DcuS uses the C4-dicarboxylate transporter DctA as a co-regulator forming DctA/DcuS sensor units. Here it is shown by fluorescence microscopy with fusion proteins that DcuS has a dynamic and preferential polar localization, even at very low expression levels. Single assemblies of DcuS had high mobility in fast time lapse acquisitions, and fast recovery in FRAP experiments, excluding polar accumulation due to aggregation. DctA and DcuR fused to derivatives of the YFP protein are dispersed in the membrane or in the cytosol, respectively, when …
Computational Modeling of Protein Dynamics in Eukaryotic Cells
2012
Proteins have important functions inside the cell, traveling diffusively or being actively transported to various cellular sites where their activity is needed. Protein motion in the cellular environment is therefore an important topic to understand. However, the cell provides a very complex environment for that motion, which poses problems especially for any modeling effort designed to interpret experimentally observed features. So as to gain a realistic picture of protein dynamics inside the cell, we have recently introduced advanced numerical methods for describing that dynamics [1]. The starting point is an accurate numerical duplicate of the cell determined by LSCM, which can be used a…
Non-structural proteins P17 and P33 are involved in the assembly of the internal membrane-containing virus PRD1.
2015
AbstractBacteriophage PRD1, which has been studied intensively at the structural and functional levels, still has some gene products with unknown functions and certain aspects of the PRD1 assembly process have remained unsolved. In this study, we demonstrate that the phage-encoded non-structural proteins P17 and P33, either individually or together, complement the defect in a temperature-sensitive GroES mutant of Escherichia coli for host growth and PRD1 propagation. Confocal microscopy of fluorescent fusion proteins revealed co-localisation between P33 and P17 as well as between P33 and the host chaperonin GroEL. A fluorescence recovery after photobleaching assay demonstrated that the diff…
Strengthening of Masonry Columns with BFRCM or with Steel Wires: An Experimental Study
2016
Nowadays, innovative materials are more frequently adopted for strengthening historical constructions and masonry structures. The target of these techniques is to improve the structural efficiency with retrofitting methods while having a reduced aesthetical impact. In particular, the use of basalt fiber together with a cementitious matrix emerges as a new technique. This kind of fiber is obtained by basalt rock without other components, and consequently it could be considered a natural material, compatible with masonry. Another innovative technique for strengthening masonry columns consists of applying steel wires in the correspondence of mortar joints. Both techniques have been recently pr…
Blanqueamiento vital domiciliario: comparación de tratamientos con peróxido de hidrógeno y peróxido de carbamida
2006
El blanqueamiento de los dientes vitales que realiza el/la paciente domiciliariamente con férulas bajo supervisión del/de la dentista es una alternativa interesante entre las que integran este tipo de terapéutica odontológica, ya sea, de forma aislada o combinada con alguna de las modalidades de tratamiento en la consulta. En este procedimiento blanqueador se usan peróxidos de baja concentración que se aplican sobre el esmalte dentario mediante una cubeta individualizada especialmente diseñada para ello. El objetivo del presente trabajo es analizar y comparar dos productos blanqueadores comerciales de diferente concentración indicados para esta técnica, VivaStyle (Vivadent) y FKD (Kin); el …
Identification of Novel Principles of Keratin Filament Network Turnover in Living Cells
2004
It is generally assumed that turnover of the keratin filament system occurs by exchange of subunits along its entire length throughout the cytoplasm. We now present evidence that a circumscribed submembranous compartment is actually the main site for network replenishment. This conclusion is based on the following observations in living cells synthesizing fluorescent keratin polypeptides: 1) Small keratin granules originate in close proximity to the plasma membrane and move toward the cell center in a continuous motion while elongating into flexible rod-like fragments that fuse with each other and integrate into the peripheral KF network. 2) Recurrence of fluorescence after photobleaching i…
Dominance of Chain Entanglement over Transient Sticking on Chain Dynamics in Hydrogen-Bonded Supramolecular Polymer Networks in the Melt
2018
The chain dynamics in supramolecular polymer networks is determined by the interplay of the kinetics of transient interchain association and relaxation of the network chains themselves. This interplay can be addressed by studying model supramolecular polymer networks in which the number of associative side groups and the molar mass of the covalently jointed backbone polymers are both varied systematically. To realize this idea, we use precursor chains with three different molar masses, which comes along with different extents of entanglement in the melt state. For each molar mass, the precursor polymers are functionalized with three different relative contents of associative side groups, gi…
Imaging of Keratin Dynamics during the Cell Cycle and in Response to Phosphatase Inhibition
2004
Publisher Summary The characterization and development of autofluorescent proteins, most prominently of the green florescent protein, have provided tools to label cellular structures such that they can be examined in living cells. This chapter highlights the potential of live cell imaging in providing novel and unprecedented insights into the dynamic organization of the keratin cytoskeleton and outlines the important aspects of this method. The live cell imaging experiments suggest that the driving force behind the vectorial and dynamic keratin distribution patterns relies both on microtubules and microfilaments and their associated factors. The studies on the dynamics of the keratin cytosk…