Search results for "Blood"

showing 10 items of 5199 documents

Tissue hypoxia in complex regional pain syndrome.

2003

Untreated complex regional pain syndrome (CRPS) may progress from acute stages with increased hair and nail growth in the affected limb to chronic stages with atrophy of the skin, muscles and bones. The aim of this study was to investigate whether tissue hypoxia could be one mechanism responsible for this late CRPS symptoms. Nineteen patients with CRPS and two control groups (healthy control subjects, surgery patients with edema) participated in this study. Skin capillary hemoglobin oxygenation (HbO(2)) was measured non-invasively employing micro-lightguide spectrophotometry (EMPHO). The EMPHO probe was mounted force-controlled onto the skin of the affected and unaffected hand. HbO(2) was m…

AdultMalePilot ProjectsAtrophyEdemaMedicineHumansReactive hyperemiaAgedSkinAnalysis of Variancebusiness.industryBlood flowOxygenationHypoxia (medical)Middle Agedmedicine.diseaseCell HypoxiaAnesthesiology and Pain MedicineComplex regional pain syndromeNeurologyAnesthesiaOxyhemoglobinsFemaleNeurology (clinical)Hemoglobinmedicine.symptombusinessComplex Regional Pain SyndromesPain
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Molecular analysis of the erythropoietin receptor system in patients with polycythaemia vera

1994

Summary Erythropoietin (EPO) is a potent regulator of the viability, proliferation and differentiation of erythroid progenitor cells. Its effect is mediated by binding to the erythropoietin receptor (EPO-R), a member of a new cytokine receptor family. Alterations of the EPO/EPO-R system have recently been shown to be involved in the pathogenesis of familial erythrocytosis and polycythaemia vera (PV). In order to define whether genetic changes in the EPO-R gene and its ligand play a role in the development of PV, the structure and expression levels of the EPO-R and EPO genes were examined in samples from bone marrow and/or peripheral blood mononuclear cells of 24 patients with PV. As expecte…

AdultMalePolycythaemiaTranscription GeneticMolecular Sequence DataBiologyPolymerase Chain ReactionPeripheral blood mononuclear cellPolycythemia veraBone Marrowhemic and lymphatic diseasesReceptors ErythropoietinmedicineHumansRNA MessengerErythropoietinPolycythemia VeraPolymorphism Single-Stranded ConformationalAgedBase SequenceHematologyMiddle Agedmedicine.diseaseMolecular biologyReverse transcriptaseErythropoietin receptormedicine.anatomical_structureErythropoietinFemaleBone marrowOligonucleotide ProbesCytokine receptormedicine.drugBritish Journal of Haematology
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Leucocytosis and thrombosis at diagnosis are associated with poor survival in polycythaemia vera: a population-based study of 327 patients

2012

Three hundred and twenty-seven patients from two population-based cohorts with an established diagnosis of polycythaemia vera were studied for prognostic risk factors for survival and leukaemia in a long-term survey. The relative survival (RS) was 72% and 46% at 10 and 20 years respectively, from the time of diagnosis. Multivariate analysis identified age >70 years, white blood cell count >13 × 10(9) /l and thrombo-embolism at diagnosis as independent risk factors. Patients with two or three of these factors had a 10 year RS of 26%, compared with 59% and 84% in patients with one and no risk factors, respectively. Age and leucocyte count are the main predicting factors for survival in polycy…

AdultMalePolycythaemiamedicine.medical_specialtyLeukocytosisPopulationComorbidityKaplan-Meier EstimateLeukocyte CountYoung AdultPolycythemia veraRisk FactorsCause of DeathNeoplasmshemic and lymphatic diseasesWhite blood cellInternal medicineEpidemiologymedicineHumansRisk factoreducationPolycythemia VeraAgedProportional Hazards ModelsAged 80 and overHeart FailureSwedeneducation.field_of_studyRelative survivalProportional hazards modelbusiness.industryThrombosisHematologyMiddle AgedPrognosismedicine.diseaseSurgeryLeukemia Myeloid Acutemedicine.anatomical_structureDisease ProgressionFemaleFrancebusinessFollow-Up StudiesBritish Journal of Haematology
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Polymorphism of C'3 in German, Bulgarian, Iranian and Angola population

1973

Using agarose gel electrophoresis, C'3 was typed in 4 populations. The gene frequencies of the common allele C'3S in these 4 populations were Germans (0.8071), Bulgarians (0.8149), Iranians (0.7920) and Angola (0.9532). The results are discussed.

AdultMalePopulationBeta-GlobulinsIranBiologyGermanGene FrequencyPolymorphism (computer science)GeneticsHumansBulgarianAlleleBulgariaeducationGeneAllelesGenetics (clinical)Geneticseducation.field_of_studyPolymorphism GeneticGermany WestBlood Protein Electrophoresislanguage.human_languageHuman geneticsPhenotypeAngolaAgarose gel electrophoresislanguageFemaleHuman Genetics
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Associations between leprosy and serum protein groups

1970

Serum protein group typings were done on a sample of n=173 adult healthy Negroes from the Macua tribe in Mozambique and on n=129 leprous individuals from the same tribe. Unfortunately, the type of leprosy is not known. Whilst between leprosy and haptoglobins, transferrins and Pi proteins no associations could be observed, statistically significant associations between leprosy and ceruloplasmin resp. β2 I were found. It is pointed out that these associations should be confirmed by further research on other populations and with respect to type and process of leprosy, until biological and population genetical interpretations will be possible.

AdultMalePopulationSerum proteinBlack PeopleTribe (biology)LeprosyGeneticsmedicineHumansAlleleMetabolic diseaseeducationAllelesMozambiqueGenetics (clinical)Glycoproteinseducation.field_of_studyHaptoglobinsbiologyHaptoglobinTransferrinCeruloplasminfood and beveragesmedicine.diseaseBlack or African AmericanGenetics PopulationPhenotypeImmunologyBlood Group Antigensbiology.proteinFemaleLeprosyTrypsin InhibitorsCeruloplasminHuman Genetics
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Translational value of choroid plexus imaging for tracking neuroinflammation in mice and humans.

2021

Neuroinflammation is a pathophysiological hallmark of multiple sclerosis and has a close mechanistic link to neurodegeneration. Although this link is potentially targetable, robust translatable models to reliably quantify and track neuroinflammation in both mice and humans are lacking. The choroid plexus (ChP) plays a pivotal role in regulating the trafficking of immune cells from the brain parenchyma into the cerebrospinal fluid (CSF) and has recently attracted attention as a key structure in the initiation of inflammatory brain responses. In a translational framework, we here address the integrity and multidimensional characteristics of the ChP under inflammatory conditions and question w…

AdultMaleProteomicsEncephalomyelitis Autoimmune ExperimentalMultiple SclerosisMiceNatalizumabCerebrospinal fluidImmune systemmedicineAnimalsHumansNeuroinflammationMultidisciplinarybusiness.industryMultiple sclerosisExperimental autoimmune encephalomyelitisNeurodegenerationBrainmedicine.diseaseMagnetic Resonance ImagingMice Inbred C57BLDisease Models AnimalBlood-Brain BarrierChoroid PlexusNeuroinflammatory DiseasesChoroid plexusFemalebusinessNeurosciencemedicine.drugProceedings of the National Academy of Sciences of the United States of America
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Serum Levels of Soluble CD23 in Patients with Asthma or Rhinitis Monosensitive to Parietaria. Its Relation to Total Serum IgE levels and Eosinophil C…

1999

The diagnostic value for allergies of the low affinity IgE receptor and its soluble circulating fragment (sCD23) remains unclear. In particular, little is know about seasonal influences on serum sCD23 levels in subjects with pollen allergy. In the present study, to gain insight into pathophysiological role of sCD23, we have analyzed, in blood from patients allergic to Parietaria sCD23, IgE, and eosinophil cationic protein (ECP) serum levels. IgE were assessed as atopy markers and ECP as an inflammation marker. Patients were studied during and out of pollen season, and results were compared to those obtained in nonallergic subjects. The study population included 42 nonsmoking outpatients, li…

AdultMalePulmonary and Respiratory MedicineAllergyParietariaAdolescentImmunoglobulin EAtopyRibonucleasesHumansImmunology and AllergyMedicinePlant ProteinsAsthmaEosinophil cationic proteinbiologyReceptors IgEbusiness.industryCD23Rhinitis Allergic SeasonalBlood ProteinsGeneral MedicineAllergensEosinophil Granule ProteinsImmunoglobulin EMiddle Agedmedicine.diseasebiology.organism_classificationAsthmaSolubilityImmunologybiology.proteinPollenPopulation studyFemaleImmunizationInflammation MediatorsbusinessAllergy and Asthma Proceedings
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Shear Stress and Aortic Strain Associations With Biomarkers of Ascending Thoracic Aortic Aneurysm

2019

This study aims to investigate the association of wall shear stress (WSS) and aortic strain with circulating biomarkers including matrix metalloproteinases (MMP), tissue inhibitors of metalloproteinase (TIMP), and exosomal level of microRNA (miRNA) in ascending aortic aneurysms of patients with bicuspid or tricuspid aortic valve.A total of 76 variables from 125 patients with ascending aortic aneurysms were collected from (1) blood plasma to measure plasma levels of miRNAs and protein activity; (2) computational flow analysis to estimate peak systolic WSS and time-average WSS (TAWSS); and (3) imaging analysis of computed tomography angiography to determine aortic wall strain. Principal compo…

AdultMalePulmonary and Respiratory MedicineAortic valvemedicine.medical_specialtybicuspid aortic valveVascular Remodeling030204 cardiovascular system & hematologyMechanotransduction CellularThoracic aortic aneurysm03 medical and health sciencesAortic aneurysm0302 clinical medicineBicuspid aortic valveBicuspid Aortic Valve Diseaseascending thoracic aortic aneurysmMatrix Metalloproteinase 12medicine.arteryInternal medicineAscending aortamedicineHumansAortaAgedComputed tomography angiographyAortaTissue Inhibitor of Metalloproteinase-1Aortic Aneurysm Thoracicmedicine.diagnostic_testbusiness.industrybiomarkersSettore ING-IND/34 - Bioingegneria IndustrialeMiddle Agedmedicine.diseaseMicroRNAsLogistic ModelsBlood pressuremedicine.anatomical_structure030228 respiratory systemAortic Valvecardiovascular systemCardiologyFemaleSurgeryStress MechanicalMatrix Metalloproteinase 1Cardiology and Cardiovascular MedicinebusinessThe Annals of Thoracic Surgery
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Baroreflex control of heart rate during sleep in severe obstructive sleep apnoea: effects of acute CPAP

2006

Baroreflex control of heart rate during sleep (baroreflex sensitivity; BRS) has been shown to be depressed in obstructive sleep apnoea (OSA), and improved after treatment with continuous positive airway pressure (CPAP). Whether CPAP also acutely affects BRS during sleep in uncomplicated severe OSA is still debatable. Blood pressure was monitored during nocturnal polysomnography in 18 patients at baseline and during first-time CPAP application. Spontaneous BRS was analysed by the sequence method, and estimated as the mean sequence slope. CPAP did not acutely affect mean blood pressure or heart rate but decreased cardiovascular variability during sleep. Mean BRS increased slightly during CPAP…

AdultMalePulmonary and Respiratory MedicineBaroreceptorPolysomnographymedicine.medical_treatmentPositive pressureBaroreflexHeart RateHeart rateHumansMedicineContinuous positive airway pressureMechanical ventilationAnalysis of VarianceSleep Apnea ObstructiveContinuous Positive Airway Pressurebusiness.industryBaroreflexMiddle Agedrespiratory tract diseasesMean blood pressureBlood pressurebaroreceptors blood pressure hypoxia positive intrathoracic pressure sleepAnesthesiaLinear ModelsMED/09 - MEDICINA INTERNAbusinesscirculatory and respiratory physiologyEuropean Respiratory Journal
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Budesonide increases TLR4 and TLR2 expression in Treg lymphocytes of allergic asthmatics

2015

Abstract Background Reduced innate immunity responses as well as reduced T regulatory activities characterise bronchial asthma. Objectives In this study the effect of budesonide on the expression of TLR4 and TLR2 in T regulatory lymphocyte sub-population was assessed. Methods TLR4 and TLR2 expression in total peripheral blood mononuclear cells (PBMC), in CD4+/CD25+ and in CD4+/CD25− was evaluated, by flow cytometric analysis, in mild intermittent asthmatics (n = 14) and in controls (n = 11). The in vitro effects of budesonide in modulating: TLR4 and TLR2 expression in controls and in asthmatics; IL-10 expression and cytokine release (IL-6 and TNF-α selected by a multiplex assay) in asthmati…

AdultMalePulmonary and Respiratory MedicineBudesonidemedicine.medical_treatmentLymphocyteIn Vitro TechniquesCorticosteroids; Immunoregulation; T lymphocytes; TLR; Adult; Asthma; Budesonide; Cytokines; Female; Flow Cytometry; Glucocorticoids; Humans; In Vitro Techniques; Interleukin-10; Leukocytes Mononuclear; Male; T-Lymphocytes Regulatory; Toll-Like Receptor 2; Toll-Like Receptor 4; Young Adult; Pulmonary and Respiratory Medicine; Pharmacology (medical); Biochemistry (medical); Medicine (all)T-Lymphocytes RegulatoryPeripheral blood mononuclear cellYoung AdultGlucocorticoidTLRT lymphocytemedicineCorticosteroidHumansPharmacology (medical)IL-2 receptorBudesonideCytokineGlucocorticoidsIn Vitro Techniquebusiness.industryMedicine (all)Biochemistry (medical)ImmunoregulationFlow CytometryAsthmaToll-Like Receptor 2Interleukin-10Toll-Like Receptor 4Interleukin 10TLR2Cytokinemedicine.anatomical_structureImmunologyLeukocytes MononuclearTLR4CytokinesFemalebusinessHumanmedicine.drugPulmonary Pharmacology & Therapeutics
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