Search results for "CAG"

Effect of cadmium on growth, isoflavonoid and protein accumulation patterns in mycorrhizal roots of Medicago truncatula

2007

International audience; Ecosystems are submitted to various abiotic stresses, among which heavy metals represent major industrial pollutants. Cadmium (Cd), that has damaging effects on plant metabolism, occurs in agricultural environments through industrial pollution and human activities, including phosphate fertiliser and sewage sludge applications. Metal availability to plants can be modulated by soil microorganisms, including arbuscular mycorrhizal (AM) fungi. In the present work, Cd effects on the model legume Medicago truncatula inoculated or not with the AM fungus Glomus intraradices have been studied at 3 levels: (1) plant biomass production together with green part chlorophyll quant…

Achievements from GLIP functional genomics platforms

2008

Format du poster : N° W212  Format du poster : N° W212; absent

Arbuscular mycorhizal proteomes: what news at the nearby and distant horizon?

2007

International audience; Proteomics has soon emerged as a powerful tool to point out protein modifications in roots interacting with arbuscular mycorrhiza (AM) fungi. Depending on the developmental mycorrhizal stage and on the available amount of mycorrhizal material, untargeted and/or sub-cellular proteomic approaches were applied to reveal and identify proteins whose accumulation was modified during the AM colonisation of Medicago truncatula roots. For the early stage of symbiosis, the protein patterns obtained from noninoculated roots and roots synchronized for appressorium formation in wild-type (Jemalong J5), penetration-defective (TRV25, dmi3) and autoregulation-defective (TR122, sunn)…

Plant gene responses to Glomus mosseae in compatible and incompatible genotypes of Medicago truncatula

2006

International audience

Could subcellular proteomics of root plastids teach us more about mycorrhizal symbiosis?

2007

International audience; The arbuscular mycorrhizal (AM) symbiosis is a mutualistic association between soil-borne fungi and the roots of most plant species. Involving the bilateral exchange of nutrients, the symbiosis is connected to drastic changes in plant cell organelle morphology and physiology. Root plastids, in particular, are forming extensive, network-like structures covering the main symbiotic interface, i.e., intracellular, highly branched haustorium-like fungal structures called arbuscules. These plastid networks are highly dynamic and are formed and degraded concomitantly with the formation and degradation of arbuscules. By producing basic metabolic building blocks like fatty ac…

Vers la validation fonctionnelle du gène WEE1 dans l’acquisition de la résistance au stress abiotique chez des protéagineux

2017

Plant genotype: a lever in the interactions between plant and its associated rhizosphere microbiome

2018

International audience; In the context of highly pressured agricultural production using low levels of inputs, cropping strategies could take advantage of existing plant-microbiome interactions that can improve both plant growth and health. We believe that plant genetics can provide some clues to better understand the mechanisms underlying microbiome recruitment by the plant.The aims of this work were (i) to assess plant genotype effect on the rhizosphere bacterial communities in relation to the plant nutritional strategies for a core collection of 155 genotypes of Medicago truncatula and (ii) to highlight the genetic determinisms potentially associated in these interactions. To achieve the…

Genes encoding transcription factors in Glomus intraradices and their expression at the appressoria stage of arbuscular mycorrhiza interactions

2007

International audience; Molecular pathways governing the life cycle of arbuscular mycorrhizal (AM) fungi and their symbiotic interactions with root tissues are not yet fully understood. Most studies fo fungal responses to host plants have targeted developmental stages before root contact (germinating spores), or after root colonization (intraradical mycelium). We are focusing on the early cell events of appressoria contact with the root surface which are essential to the successful outcome of the AM symbiosis. Recent monitoring of Glomus intraradices gene expression at this stage has revealed differential fungal responses to roots of host and non-host (Myc- mutants) M. truncatula (Seddas et…

Effect of Arbuscular Mycorrhiza establishment on the genetic structure and functionality of bacterial communities in the rhizosphere of Medicago trun…

2007

Bacterial communities associated with (i) M. Truncatula J5 (Myc+/Nod+) and TRV48 (Myc+/Nod-), and with (ii) M. Truncatula TRV25 (Myc-/Nod-) were compared. The genetic structure of communities was characterized with an A-RISA DNA fingerprint. The genetic structures of communities associated with mycorrhizal and non-mycorrhizal roots were significantly different. Bacteria belonging to the Oxalobacteraceae and Comamonadaceae families were preferentially associated with mycorrhizal roots. The genetic diversity of cultured bacteria belonging to these groups was analysed by BOX-PCR. The diversity of isolates from mycorrhizal and non-mycorrhizal roots was significantly different. The functionality…

Protein storage accumulation at mid-embryogenesis in Medicago truncatula and Pisum sativum

2005

National audience