Search results for "CAPE"
showing 10 items of 1938 documents
Towards (dis)continuity of agricultural wetlands: Latvia’s polder landscapes after Soviet productivism
2017
The concepts of agricultural regimes in advanced economies, such as productivism or non/neo/post-productivism, have been critically debated over the last decades to understand the transition and diversity of modern agriculture. We explore these concepts to understand the environmentally vulnerable landscape of agricultural wetlands in Latvia that, during the era of Soviet high modernism (productivist agricultural regime), have been converted into polders as part of a mass drainage movement. Today, these post-Soviet agro-polders can be characterised as antipodes in relation to integrity of heritage, ecology and the socio-economics of agricultural concerns. Building on case studies, wider pol…
Met inhibition revokes IFNγ-induction of PD-1 ligands in MET-amplified tumours
2019
BACKGROUND: Interferon-induced expression of programmed cell death ligands (PD-L1/PD-L2) may sustain tumour immuneevasion. Patients featuring MET amplification, a genetic lesion driving transformation, may benefit from anti-MET treatment. We explored if MET-targeted therapy interferes with Interferon-gamma modulation of PD-L1/PD-L2 in MET-amplified tumours.METHODS: PD-L1/PD-L2 expression and signalling pathways downstream of MET or Interferon-gamma were analysed in MET-amplified tumour cell lines and in patient-derived tumour organoids, in basal condition, upon Interferon-gamma stimulation, and after anti-MET therapy.RESULTS: PD-L1 and PD-L2 were upregulated in MET-amplified tumour cells up…
Form, figure and landscape for nature in the city.
2014
The article “Form, Figure and Landscape for Nature in the city” describes, through a sequence of steps, the urban and territorial threshold that give structure to the Piacenza southern area. It analyzes in detail the elements that define three “rooms” at different scales: - at the territorial scale, the farmhouses define a system of elements as focal point overlooking the agricultural surface, interpreted as background; - at the urban scale, the reconfiguration of the morphological tissue of the INA Casa neighborhoods allows to read a district itself as a specific “room” inside Piacenza urban fabric; - at the architectural scale, the gardens of interior courtyards of via Francigena building…
Il paesaggio come materia fondativa del progetto
2017
Il contributo affronta il tema del rapporto tra architettura e natura attraverso il ruolo strutturante che il paesaggio antropizzato riveste per quel tipo di progetto che basa la propria essenza costitutiva sulla dimensione fisica e spirituale dei luoghi; in tal senso, il paesaggio è inteso quale finalità principale dell’architettura. Ad esplicitazione dei concetti espressi, si prendono in esame gli esiti della ricerca progettuale condotta dall’autore nell’ambito del proprio laboratorio di progettazione architettonica, destinato allo sviluppo delle tesi di laurea, incentrato sul tema L’architettura nella roccia e operante intorno ai concetti dello scavo, della sottrazione, del progetto del …
Unraveling the SARS-CoV-2 Main Protease Mechanism Using Multiscale DFT/MM Methods
2020
<p>We present a detailed theoretical analysis of the reaction mechanism of proteolysis catalyzed by the main protease of SARS-CoV-2. Using multiscale simulation methods, we have characterized the interactions stablished by a peptidic substrate in the active site and then we have explored the free energy landscape associated to the acylation and de-acylation steps of the proteolysis reaction, characterizing the transition states of the process. Our mechanistic proposals can explain most of the experimental observations made on the highly similar ortholog protease of SARS-CoV. We point out to some key interactions that may facilitate the acylation process and thus can be crucial in the …
Reconstructing the free-energy landscape of Met-enkephalin using dihedral principal component analysis and well-tempered metadynamics
2013
Well-Tempered Metadynamics (WTmetaD) is an efficient method to enhance the reconstruction of the free-energy surface of proteins. WTmetaD guarantees a faster convergence in the long time limit in comparison with the standard metadynamics. It still suffers however from the same limitation, i.e. the non trivial choice of pertinent collective variables (CVs). To circumvent this problem, we couple WTmetaD with a set of CVs generated from a dihedral Principal Component Analysis (dPCA) on the Ramachadran dihedral angles describing the backbone structure of the protein. The dPCA provides a generic method to extract relevant CVs built from internal coordinates. We illustrate the robustness of this …
Curvature and Torsion of Protein Main Chain as Local Order Parameters of Protein Unfolding
2020
International audience; Thermal protein unfolding resembles a global (two-state) phase transition. At the local scale, protein unfolding is, however, heterogeneous and probe dependent. Here, we consider local order parameters defined by the local curvature and torsion of the protein main chain. Because chemical shift (CS) measured by NMR spectroscopy is extremely sensitive to the local atomic environment, CS has served as a local probe of thermal unfolding of proteins by varying the position of the atomic isotope along the amino-acid sequence. The variation of the CS of each C(alpha) atom along the sequence as a function of the temperature defines a local heat-induced denaturation curve. We…
Unraveling the SARS-CoV-2 Main Protease Mechanism Using Multiscale Methods
2020
We present a detailed theoretical analysis of the reaction mechanism of proteolysis catalyzed by the main protease of SARS-CoV-2. Using multiscale simulation methods, we have characterized the interactions established by a peptidic substrate in the active site, and then we have explored the free energy landscape associated with the acylation and deacylation steps of the proteolysis reaction, characterizing the transition states of the process. Our mechanistic proposals can explain most of the experimental observations made on the highly similar ortholog protease of SARS-CoV. We point to some key interactions that may facilitate the acylation process and thus can be crucial in the design of …
Préparation distancielle au CAPEPS externe : un objectif commun, des expériences différentes
2021
International audience
New Interpretative Scales for Lichen Bioaccumulation Data: The Italian Proposal
2019
The interpretation of lichen bioaccumulation data is of paramount importance in environmental forensics and decision-making processes. By implementing basic ideas underlying previous interpretative scales, new dimensionless, species-independent &ldquo