Search results for "CCC"

Update of the statements on biology and clinical impact of occult hepatitis B virus infection

2019

In October 2018 a large number of international experts with complementary expertise came together in Taormina to participate in a workshop on occult hepatitis B virus infection (OBI). The objectives of the workshop were to review the existing knowledge on OBI, to identify issues that require further investigation, to highlight both existing controversies and newly emerging perspectives, and ultimately to update the statements previously agreed in 2008. This paper represents the output from the workshop.

2020

ObjectiveThe HBV HBx regulatory protein is required for transcription from the covalently closed circular DNA (cccDNA) minichromosome and affects the epigenetic control of both viral and host cellular chromatin.DesignWe explored, in relevant cellular models of HBV replication, the functional consequences of HBx interaction with DLEU2, a long non-coding RNA (lncRNA) expressed in the liver and increased in human hepatocellular carcinoma (HCC), in the regulation of host target genes and the HBV cccDNA.ResultsWe show that HBx binds the promoter region, enhances the transcription and induces the accumulation of DLEU2 in infected hepatocytes. We found that nuclear DLEU2 directly binds HBx and the…

Customised next-generation sequencing multigene panel to screen a large cohort of individuals with chromatin-related disorder

2020

BackgroundThe regulation of the chromatin state by epigenetic mechanisms plays a central role in gene expression, cell function, and maintenance of cell identity. Hereditary disorders of chromatin regulation are a group of conditions caused by abnormalities of the various components of the epigenetic machinery, namely writers, erasers, readers, and chromatin remodelers. Although neurological dysfunction is almost ubiquitous in these disorders, the constellation of additional features characterizing many of these genes and the emerging clinical overlap among them indicate the existence of a community of syndromes. The introduction of high-throughput next generation sequencing (NGS) methods f…

CTCF and BORIS Regulate Rb2/p130 Gene Transcription: A Novel Mechanism and a New Paradigm for Understanding the Biology of Lung Cancer

2011

Abstract Although innumerable investigations regarding the biology of lung cancer have been carried out, many aspects thereof remain to be addressed, including the role played by the retinoblastoma-related protein Rb2/p130 during the evolution of this disease. Here we report novel findings on the mechanisms that control Rb2/p130 gene expression in lung fibroblasts and characterize the effects of Rb2/p130 deregulation on the proliferative features of lung cancer cells. We revealed for the first time that in lung fibroblasts the expression of Rb2/p130 gene is directly controlled by the chromatin insulator CCCTC-binding factor, CTCF, which by binding to the Rb2/p130 gene promoter induces, and/…

7C: Computational Chromosome Conformation Capture by Correlation of ChIP-seq at CTCF motifs.

2019

Abstract Background Knowledge of the three-dimensional structure of the genome is necessary to understand how gene expression is regulated. Recent experimental techniques such as Hi-C or ChIA-PET measure long-range chromatin interactions genome-wide but are experimentally elaborate, have limited resolution and such data is only available for a limited number of cell types and tissues. Results While ChIP-seq was not designed to detect chromatin interactions, the formaldehyde treatment in the ChIP-seq protocol cross-links proteins with each other and with DNA. Consequently, also regions that are not directly bound by the targeted TF but interact with the binding site via chromatin looping are…

Cine epistolar: imaginación y política del intercambio estético

2014

Resumen: Este articulo estudia las implicaciones politicas del intercambio estetico, mediante el estudio de un proyecto de correspondencias filmicas desarrollado por el Centro de Cultura Contemporanea de Barcelona (CCCB), entre 2005 y 2011. Propone el intercambio epistolar como una forma de interpelacion cinematografica capaz de crear afinidades geopoliticas a traves de la puesta en comun y el ensamblaje de realidades sensibles. En definitiva, el objetivo del presente estudio es investigar el potencial del intercambio estetico como ejercicio de imaginacion politica, planteando la posibilidad de un valor de uso de la imagen mas alla de la economia politica neoliberal. Abstract: This article …

Challenges in EU External Climate Change Policy-Making in the Early Post-Lisbon Era: The UNFCCC Copenhagen Negotiations

2011

The 15th United Nations Framework Convention on Climate Change (UNFCCC) Conference of the Parties (COP) meeting held in Copenhagen from 7 to 18 December 2009, which took place one week after the entry into force of the Treaty of Lisbon on 1 December 2009, has brought about rather disappointing outcomes from the perspective of the European Union (EU), which had previously displayed substantial leadership within the UN climate regime. Contrary to the EU’s objectives for the COP15 meeting, no legally binding agreement was reached to succeed the Kyoto Protocol after 2012 and the final Copenhagen Accord contained disappointingly few ambitious targets. This chapter tries to explain how this resul…

Structure and function of the vacuolar Ccc1/VIT1 family of iron transporters and its regulation in fungi

2020

Iron is an essential micronutrient for most living beings since it participates as a redox active cofactor in many biological processes including cellular respiration, lipid biosynthesis, DNA replication and repair, and ribosome biogenesis and recycling. However, when present in excess, iron can participate in Fenton reactions and generate reactive oxygen species that damage cells at the level of proteins, lipids and nucleic acids. Organisms have developed different molecular strategies to protect themselves against the harmful effects of high concentrations of iron. In the case of fungi and plants, detoxification mainly occurs by importing cytosolic iron into the vacuole through the Ccc1/V…

The EU’s Role in International Climate Change Policy-Making: A Global Leader in Decline?

2013

This chapter assesses the European Union’s (EU) role in international climate change policy-making by comparing the EU’s degree of goal attainment at the United Nations Framework Convention on Climate Change (UNFCCC) Conference of the Parties (COP) negotiations in Copenhagen in 2009 and Cancun in 2010. By making use of three analytical factors (coherence, the opportunity structure and politicisation) we assess the outcomes of both negotiations for the EU. The Durban negotiations of 2011 are touched upon in the conclusions.

Hepatitis B virus maintains its pro-oncogenic properties in the case of occult HBV infection.

2003

Background & Aims: Occult hepatitis B virus (HBV) infection is characterized by persistence of HBV DNA into the tissue of hepatitis B surface antigen-negative individuals. The clinical relevance of this peculiar infection is still under debate. In particular, the impact of occult HBV infection in cases of hepatocellular carcinoma (HCC) is uncertain. We investigated the prevalence and molecular status of occult HBV in patients with HCC. Methods: We tested tumor tissues from 107 patients with HCC and the corresponding nontumor liver tissue from 72 of these patients for HBV DNA. We also examined liver specimens from 192 patients with chronic hepatitis. All cases were hepatitis B surface antige…