Search results for "CD163"

showing 8 items of 8 documents

The two faces of tumor-associated macrophages and their clinical significance in colorectal cancer

2019

© 2019 Pinto, Rios, Durães, Ribeiro, Machado, Mantovani, Barbosa, Carneiro and Oliveira. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

0301 basic medicineMaleColorectal cancerprognostic and tumor relapseMacrophages / immunologyhuman macrophage surface markers0302 clinical medicineTumor MicroenvironmentImmunology and AllergyColorectal Neoplasms / mortalityOriginal ResearchAged 80 and overTumor immunomodulationCD68tumor-associated macrophagesTumor-associated macrophagesTumor Microenvironment / immunologyMiddle AgedPrognosis3. Good healthMacrophage polarizationImmunohistochemistryFemaleHuman macrophage surface markerstumor immunomodulationColorectal NeoplasmsInfiltration (medical)lcsh:Immunologic diseases. AllergyAdultmacrophage polarizationImmunologyMacrophage polarizationcolorectal cancerBiologyPrognostic and tumor relapseColorectal Neoplasms / pathology03 medical and health sciencesYoung AdultImmune systemmedicineHumansAgedMacrophagesColorectal Neoplasms / immunologymedicine.diseaseColorectal cancer030104 developmental biologyCancer researchlcsh:RC581-607CD163CD80030215 immunology
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2021

Background: Immunomodulatory properties of bisphosphonates (BP) are suggested to contribute to the development of medication-associated osteonecrosis of the jaw (MRONJ). Furthermore, bisphosphonate-derived immune modulation might contribute to the anti-metastatic effect observed in breast cancer patients. Macrophages are potential candidates for the mediation of immunomodulatory effects of bisphosphonates. The study aimed to investigate the influence of bisphosphonates alone and in combination with surgical trauma on systemic macrophage polarization (M1 vs. M2) using an in vivo rat model. Methods: A total of 120 animals were divided into four groups. Groups 2 and 4 were treated with 8 × 40 …

0301 basic medicinemedicine.medical_treatmentMacrophage polarizationSpleenPharmacologyCatalysisInorganic Chemistry03 medical and health sciences0302 clinical medicineIn vivomedicinePhysical and Theoretical ChemistryMolecular BiologySpectroscopyLungCD68business.industryOrganic ChemistryGeneral MedicineBisphosphonatemedicine.diseaseComputer Science Applications030104 developmental biologymedicine.anatomical_structure030220 oncology & carcinogenesisOsteonecrosis of the jawbusinessCD163International Journal of Molecular Sciences
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Macrophage phenotype in the subclinical gut inflammation of patients with ankylosing spondylitis

2014

OBJECTIVE: Long-term evolution of subclinical gut inflammation to overt Crohn's disease (CD) has been described in AS patients. The aim of this study was to evaluate macrophage polarization occurring in the inflamed gut of patients with AS. METHODS: Twenty-seven HLA-B27(+) AS patients, 20 CD patients and 17 normal controls were consecutively enrolled. Classic M1 (iNOS(+)IL-10(-)), resolution phase (iNOS(+)IL-10(+)), M2 and CD14(+) macrophages were characterized by immunohistochemistry and flow cytometry. Quantitative gene expression analysis of IFN-γ, IL-4, IL-5, IL-33 and STAT6 was performed by real time PCR. RESULTS: Classic M1 macrophages were expanded in CD and AS, where resolution phas…

AdultMalePathologymedicine.medical_specialtymedicine.medical_treatmentCD14BiopsyMacrophage-activating factorMacrophage polarizationInflammationReal-Time Polymerase Chain ReactionM2 macrophageYoung AdultRheumatologyIleumMedicineMacrophageHumansPharmacology (medical)Spondylitis AnkylosingAgedbusiness.industryMacrophagesresolution phase macrophagesDNAIleitisMiddle AgedFlow CytometryImmunohistochemistryInterleukin 10Settore MED/16 - Reumatologiaankylosing spondylitiCytokinePhenotypeGene Expression RegulationM1 macrophages M2 macrophages ankylosing spondylitis gut inflammation interleukin 33 resolution phase macrophagesImmunologyCytokinesFemalegut inflammationinterleukin 33medicine.symptombusinessCD163M1 macrophage
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Human Embryonic Stem Cell-Derived and Fetal Macrophages Exhibit Mainly Features of Alternative (M2) Polarization.

2009

Abstract Abstract 4598 Monocyte heterogeneity has long been recognized and 2 functional subsets of human monocytes that exert specific roles in homeostasis and inflammation in vivo, M1 and M2 monocytes, has been described. The different monocyte subsets seem to reflect developmental stages with distinct physiological roles but few is known whether the macrophage diversity arises in early ontogeny. Human embryonic stem cells (hESC) provide an unique model for in vitro studies of the early ontogeny of the hematopoietic system. Human embryonic monocytes were obtained from embryoid bodies cultured for 3 weeks in the presence of BMP4, VEGF and a mixture of hematopoietic cytokines. The sorted CD1…

ChemokinebiologyCD14MonocyteImmunologyMacrophage polarizationCell BiologyHematologyEmbryoid bodyBiochemistryEmbryonic stem cellCell biologymedicine.anatomical_structureImmunologybiology.proteinmedicineMacrophageCD163Blood
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PD-1, PD-L1, and CD163 in pancreatic undifferentiated carcinoma with osteoclast-like giant cells: A expression patterns and clinical implications

2018

Undifferentiated carcinoma with osteoclast-like giant cells (UCOGC), a variant of pancreatic ductal adenocarcinoma (PDAC), has a striking genetic similarity to PDAC but a significantly improved overall survival. We hypothesize that this difference could be due to the immune response to the tumor, and as such, we investigated the expression of PD-1, PD-L1, and CD163 in a series of UCOGC. To this aim, 27 pancreatic UCOGCs (11 pure and 16 PDAC-associated), 5 extrapancreatic tumors with osteoclast-like giant cells and 10 pancreatic anaplastic carcinomas were immunostained using antibodies against PD-1, PD-L1, and CD163. In pancreatic UCOGCs, PD-L1 was expressed in neoplastic cells of 17 (63%) o…

Male0301 basic medicineIndianaProgrammed Cell Death 1 ReceptorOsteoclast; PDAC; Pancreatic Cancer; Tumor-Associated Macrophages; UCOGCOsteoclastsGiant CellsB7-H1 Antigen0302 clinical medicineTumor-Associated MacrophagesTumours of the digestive tract Radboud Institute for Molecular Life Sciences [Radboudumc 14]LymphocytesAged 80 and overbiologyTumor-associated macrophagesCell DifferentiationMiddle AgedOsteoclast; Pancreatic cancer; PDAC; Tumor-associated macrophages; UCOGC; 2734ImmunohistochemistryEuropePhenotypemedicine.anatomical_structure030220 oncology & carcinogenesisOsteoclastFemaleAntibodyCarcinoma Pancreatic DuctalAdult2734Antigens Differentiation MyelomonocyticReceptors Cell SurfaceUCOGCPathology and Forensic MedicinePancreatic Cancer03 medical and health sciencesImmune systemAll institutes and research themes of the Radboud University Medical CenterAntigens CDOsteoclastPD-L1Pancreatic cancerBiomarkers TumormedicineHumansHistiocyteAgedNeoplasm StagingPDACHistiocytesPancreatic cancermedicine.diseasePancreatic Neoplasms030104 developmental biologyGiant cellCancer researchbiology.proteinCD163
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IL-33 is overexpressed in the inflamed arteries of patients with giant cell arteritis.

2013

OBJECTIVE: To study the expression of interleukin (IL)-33 and to evaluate its relationship with macrophage polarisation in artery biopsy specimens from patients with giant cell arteritis (GCA). METHODS: IL-33, ST2, p-STAT-6 and perivascular IL-1 receptor-associated kinase 1 (p-IRAK1) tissue distribution was evaluated by immunohistochemistry. Inducible nitric oxide synthase and CD163 were also used by immunohistochemistry to evaluate the M1 and M2 polarisation, respectively. Quantitative gene expression analysis of IL-33, T-helper (Th)2-related transcription factor STAT6, Th2 cytokines (IL-4, IL-5, IL-25) and interferon (IFN)-γ was performed in artery biopsy samples obtained from 20 patients…

MalePathologymedicine.medical_specialtyImmunologyGiant Cell ArteritisInflammationGeneral Biochemistry Genetics and Molecular BiologyGiant cell arteritis IL-33 macrophagesRheumatologyGiant cell arteritiImmunology and AllergyMedicineHumansReceptorSTAT6AgedAged 80 and overInflammationAged; Aged 80 and over; Female; Giant Cell Arteritis; Humans; Immunohistochemistry; Inflammation; Interleukins; Male; Middle Aged; Reverse Transcriptase Polymerase Chain Reaction; Temporal Arteries; Transcriptomebusiness.industryReverse Transcriptase Polymerase Chain ReactionInterleukinsInterleukinMiddle Agedmedicine.diseaseInterleukin-33ImmunohistochemistryTemporal ArteriesmacrophagesInterleukin 33Giant cell arteritisIL-33ImmunohistochemistryFemalemedicine.symptombusinessTranscriptomeCD163
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The role of macrophages polarization in predicting prognosis of radically resected gastric cancer patients

2013

Tumour-associated Macrophages (TAM) present two different polarizations: classical (M1) characterized by immunostimulation activity and tumour suppression; alternative (M2) characterized by tumour promotion and immune suppression. In this retrospective study, we evaluated the correlation between the two forms of TAM with survival time in radically resected gastric cancer patients. A total of 52 chemo- and radio- naive patients were included. Two slides were prepared for each patient and double-stained for CD68/NOS2 (M1) or CD68/CD163 (M2) and five representative high-power fields per slide were evaluated for TAM count. The median value of the two macrophage populations density and the media…

Malemedicine.medical_specialtyPathologyMultivariate analysisSettore MED/06 - Oncologia MedicaKaplan-Meier EstimateGastroenterologyImmune systemM1 polarization; M2 polarization; gastric cancerGastrectomyStomach NeoplasmsInternal medicinemedicineHumanstumour associated macrophages M1 polarization M2 polarization prognostic factor gastric cancerRadical surgeryprognostic factorRetrospective Studiesbusiness.industryCD68MacrophagesM2 polarizationgastric cancerM1 polarizationCell PolarityCancerRetrospective cohort studyOriginal ArticlesCell BiologyMiddle AgedPrognosisM2 Macrophagemedicine.diseaseTreatment OutcomeMultivariate Analysistumour associated macrophagesMolecular MedicineFemalebusinessCD163Journal of Cellular and Molecular Medicine
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AB0189 Macrophages polarization in the gut of patients with ankylosing spondylitis

2013

Background Subclinical gut inflammation occurs in patients with Ankylosing Spondylitis (AS) and long term evolution to overt Crohn’s disease (CD) has been described in these patients. Gut mucosal macrophages represent the largest pool of tissue macrophages in the body. Different pathways of macrophage activation have been described in humans. Objectives To study the macrophages polarization occurring in the inflamed gut of AS patients. Methods Twenty two consecutive HLA-B27 + Ankylosing Spondylitis (AS) patients, 15 Crohn’s Disease (CD) patients and 15 normal controls were included in this study. Four AS patients developed an overt CD during the follow-up and were included. Ileal macrophage…

Pathologymedicine.medical_specialtyInnate immune systembusiness.industryCD68CD14ImmunologyAcquired immune systemGeneral Biochemistry Genetics and Molecular BiologyRheumatologyImmunologymedicineImmunology and AllergyMacrophagebusinessCD163IRF5STAT6Annals of the Rheumatic Diseases
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