Search results for "CEI"
showing 10 items of 1294 documents
Expression of Active Streptolysin O in Escherichia coli as a Maltose-Binding-Protein-Streptolysin-O Fusion Protein. The N-Terminal 70 Amino Acids are…
1996
Streptolysin 0 (SLO) is the prototype of a family of cytolysins that consists of proteins which bind to cholesterol and form very large transmembrane pores. Structure/function studies on the pore-forming cytolysin SLO have been complicated by the proteolytic inactivation of a substantial portion of recombinant SLO (rSLO) expressed in Escherichia coli. To overcome this problem, translational fusions between the E. coli maltose-binding protein (MBP) gene and SLO were constructed, using the vectors pMAL-p2 and pMAL-c2. MBP-SLO fusion proteins were degraded if secreted into the E. coli periplasm, but intact, soluble MBP-SLO fusion proteins were produced at high levels in the cytoplasm. Active S…
Cysteine-Specific Radioiodination of Proteins with Fluorescein Maleimide
1997
A protocol is described for coupling of carrier-free iodine to protein sulfhydryl groups via fluorescein maleimide. 125I is first coupled to fluorescein maleimide in the presence of chloramine T. Iodination is stopped with sodium thiosulfate, and the iodine-substituted fluorescein maleimide is reacted with free cysteines of the protein. Excess label is then removed by gel-permeation chromatography. The procedure avoids exposition of the protein to oxidative conditions and does not require purification of the labeled carrier reagent. Suitability of the method for a given protein can be evaluated spectrophotometrically without employing radioactivity. It can be applied under denaturing condit…
Xàtiva en la encrucijada. La cruz del camino de Valencia
2008
Las llamadas cruces de término raramente hacían honor al nombre con el que actualmente las conocemos; rodeaban, eso sí, el espacio habitado, erigiéndose en los principales caminos de "salida" de la población. Xátiva, la segunda ciudad durante el período foral, no se mantuvo al margen de esta costumbre y, aparte de las cruces que se conservan en el Museu de l'Almodí, hubo otras que la custodiaron desde los cuatro puntos cardinales. De todas ellas destaca la cruz del camino de Valencia, excepcional en todos los sentidos, no sólo estilísticamente, como se ha puesto de manifiesto en reiteradas ocasiones, sino también iconográficamente; teniendo en cuenta, sobre todo, que se trata de una de las …
Relationship among Perceived Dynamism, Technological Posture and Innovation Performance
2014
A main strategic election for a firm is its technological posture, which can be understood as its propensity to innovate and the moment for the introduction of technological changes into the market. This paper, which focuses on the renewable energy industry in Spain, establishes that in environments in which dynamism is understood by managers as high, and the appreciation of change is greater, firms develop a greater tendency towards technological leadership postures (pioneer or first-mover strategy) rather than conservative or follower postures (last-mover strategy). The results of the analysis, carried out through a structural equation model based on the partial least squares (PLS) techni…
Influencia de la satisfacción y el valor percibido sobre el “Word of Mouth” en los usuarios de centros deportivos
2019
Uno de los principales objetivos de los servicios deportivos, es fidelizar a los usuarios el mayor tiempo posible en dicho servicio. Por ello, desde el inicio de la literatura de la gestión del deporte, que analizan las diferentes variables de gestión, cada vez más, aparecen trabajos que tratan de aportar información relacionada con este objetivo. Dentro de las inten- ciones futuras de los usuarios, la recomendación es uno de los factores claves para poder analizar los futuros comportamientos de dichos usuarios. Por ello este trabajo, trata de analizar cuál es la influencia de la satisfacción y el valor percibido de los usuarios, sobre el “Word of Mouth” (boca-oído), es decir, como influyen…
El efecto de la experiencia de servicio emocional en las intenciones futuras del usuario de centros deportivos
2016
El presente trabajo analiza la influencia que tienen los aspectos emocionales en las intenciones futuras de los usuarios de centros deportivos. A través de una muestra compuesta por 512 usuarios de tres instalaciones deportivas privadas y diversas escalas adaptadas de valor percibido, satisfacción general, intenciones futuras y emociones, se realiza un análisis de consistencia interna de dichas escalas, un descriptivo de media y desviación estándar, análisis de correlación de Pearson y una regresión lineal múltiple. Los resultados mostraron que las escalas utilizadas eran fiables y la valoración de los usuarios fue alta en cuanto a la satisfacción y la experiencia emocional con el servicio.…
Mindfulness, perceived stress, and subjective well-being: a correlational study in primary care health professionals
2015
Background Primary health care professionals (PHPs) usually report high levels of distress and burnout symptoms related to job strain. Mindfulness, defined as non-judgmental-present-moment awareness, seems to be a moderator in the causal association between life stressors and well-being. This study aimed to verify correlations among self-reported mindfulness, perceived stress (PS), and subjective well-being (SW) in Brazilian PHPs. Methods We performed a correlational cross-sectional study in a purposive sample of Brazilian PHPs (physicians, nurses, nursing assistants, and community health workers), working in community-oriented primary care programs (known locally as “Family Health Programs…
La nuova idea di cupola : teatri sacri e profani nell’Europa d’età barocca tra storia e rappresentazione
2023
A remarkable and diverse series of ceiling structures emerged between the seventeenth and eighteenth centuries in several European cities, aiming to offer a theatrical approach to architectural space. This development unfolded amidst the widespread use of frescoed ceilings or hemispherical domes supported by drums and pendentives inspired by Roman models. Designing and building systems consisting of multiple domes, open with oculi, superimposed or surmounted by lanterns, adorned with frescoes or galleries, and featuring light and music chambers required expertise in optics, perspective, acoustics, and, in some cases, even stereotomy. These skills were necessary to transform spaces in both r…
Optic pit syndrome
1986
In a study of 15 eyes with optic pits fluorescein angiography revealed that only pits associated with serous detachment of the macular retina showed fluorescence in the late venous phase, while the pits uncomplicated by detachment remained hypofluorescent throughout the test. This finding strongly supports the hypothesis that the subretinal fluid producing the macular detachment originates from leakage of vessels located in the floor of the pit. In addition a high incidence of anomalies was found to be associated with optic pits. Therefore the author suggests to call the association between optic pit and large optic disc, parapapillary chorioretinal changes, cilioretinal vessels, situs inve…
Nash codes for noisy channels
2012
This paper studies the stability of communication protocols that deal with transmission errors. We consider a coordination game between an informed sender and an uninformed decision maker, the receiver, who communicate over a noisy channel. The sender's strategy, called a code, maps states of nature to signals. The receiver's best response is to decode the received channel output as the state with highest expected receiver payoff. Given this decoding, an equilibrium or "Nash code" results if the sender encodes every state as prescribed. We show two theorems that give sufficient conditions for Nash codes. First, a receiver-optimal code defines a Nash code. A second, more surprising observati…