Search results for "CELLULAR"

showing 10 items of 6449 documents

Impaired regulation of emotion: Neural correlates of reappraisal and distraction in bipolar disorder and unaffected relatives

2015

Deficient emotion regulation has been proposed as a crucial pathological mechanism in bipolar disorder (BD). We therefore investigated emotion regulation impairments in BD, the related neural underpinnings and their etiological relevance for the disorder. Twenty-two euthymic patients with bipolar-I disorder and 17 unaffected first-degree relatives of BD-I patients, as well as two groups of healthy gender-, age- and education-matched controls (N=22/17, respectively) were included. Participants underwent functional magnetic resonance imaging while applying two different emotion regulation techniques, reappraisal and distraction, when presented with emotional images. BD patients and relatives …

AdultMaleBipolar DisorderEmotionsPoison controlAmygdalaCellular and Molecular NeuroscienceYoung AdultCognitionFunctional neuroimagingmedicineHumansAttentionFamilyBipolar disorderBiological Psychiatrymedicine.diagnostic_testRegulation of emotionFunctional NeuroimagingBrainMiddle Agedmedicine.diseaseAmygdalaMagnetic Resonance ImagingFrontal LobePsychiatry and Mental healthmedicine.anatomical_structureSchizophreniaCase-Control StudiesOrbitofrontal cortexOriginal ArticleFemaleFunctional magnetic resonance imagingPsychologyNeuroscienceClinical psychology
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Topodiagnostic value of blink reflex R1 changes: a digital postprocessing MRI correlation study.

2001

The aim of the study was to investigate the relation of the blink reflex R1 arc to known anatomical brainstem structures. Acute vascular brainstem lesions as identified by magnetic resonance imaging (MRI) of patients with isolated R1 pathology were superimposed into a stereotactic anatomical atlas using a new method of digital postprocessing. Isolated acute brainstem lesions were documented by diffusion-weighted MRI in 12 of 24 patients with unilateral R1 pathology. The lesions were located in the ipsilateral mid- to lower pons. In three patients only, the lesion had partial contact with the principal sensory nucleus of the trigeminal nerve (PSN) on at least one level. In two patients, the …

AdultMaleBrain Stem InfarctionsPhysiologyCellular and Molecular NeurosciencePhysiology (medical)medicineImage Processing Computer-AssistedHumansCorneal reflexAgedTrigeminal nerveAged 80 and overmedicine.diagnostic_testBlinkingReflex arcSpinal trigeminal nucleusMagnetic resonance imagingAnatomyMiddle AgedMedial longitudinal fasciculusMagnetic Resonance ImagingPonsElectric Stimulationmedicine.anatomical_structureFemaleNeurology (clinical)BrainstemPsychologyBrain StemMusclenerve
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Patterns of differentiation in extraosseous Ewing's sarcoma cells. An in vitro study.

1994

BACKGROUND In vitro, tissue culture-associated differentiation assays have facilitated the identification of multiple tumor-cell types. METHODS We have investigated the capability of differentiation of three extraosseous Ewing's sarcoma cell lines toward a neural and muscular direction by in vitro stimulation with dibutyryl cyclic adenosine-monophosphate (db cAMP) and 5-azacytidine, respectively. RESULTS Elongation of cytoplasmic processes and increase of neural markers chromogranin, S-100 protein, and glial fibrillary acidic protein were observed after db cAMP treatment of these lines and neurosecretory granules as well as myelin figures were demonstrated ultrastructurally. These results s…

AdultMaleCancer ResearchPathologymedicine.medical_specialtyAdolescentCellular differentiationSoft Tissue NeoplasmsSarcoma EwingMyelinmedicineTumor Cells CulturedHumansMicroscopy Phase-ContrastbiologyGlial fibrillary acidic proteinNeural crestChromogranin AInfantCell Differentiationmedicine.diseaseImmunohistochemistryMicroscopy Electronmedicine.anatomical_structureOncologyBucladesineCell cultureAbdominal NeoplasmsCancer researchbiology.proteinAzacitidineImmunohistochemistryFemaleSarcomaCancer
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Clinical evaluation of pancreatitis-associated protein as a serum marker of hepatocellular carcinoma: Comparison with α-fetoprotein

1998

This study evaluated the significance of serum pancreatitis-associated protein (PAP) assay, as a marker of hepatocellular carcinoma (HCC), in comparison and combined with α-fetoprotein (AFP) assay. Sixty-five patients with HCC, 59 with liver cirrhosis (LC) and 68 asymptomatic controls (C) were studied. PAP and AFP values significantly increased from C to LC and HCC group (p < 0.0001). The area under receiver-operating characteristic (ROC) curve for the two markers was not statistically different. At 100% specificity, ROC analysis gave a cut-off level for AFP of 166 IU/l with 40% sensitivity, and a cut-off level of 240 µg/l for PAP with 23% sensitivity. Diagnostic accuracy of combined AFP…

AdultMaleCancer ResearchPathologymedicine.medical_specialtyCirrhosisCarcinoma HepatocellularPancreatitis-Associated ProteinsAsymptomaticSensitivity and SpecificityAntigens NeoplasmBiomarkers TumorMedicineHumansLectins C-TypeneoplasmsTumor markerAgedbusiness.industryLiver NeoplasmsGeneral MedicineMiddle Agedmedicine.diseasedigestive system diseasesLogistic ModelsOncologyHepatocellular carcinomaPancreatitisFemalealpha-Fetoproteinsmedicine.symptombusinessClinical evaluationSerum markersAcute-Phase Proteins
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Does access to care play a role in liver cancer survival? The ten-year (2006–2015) experience from a population-based cancer registry in Southern Ita…

2021

Abstract Background Hepatocellular carcinoma (HCC) is the most frequent primary invasive cancer of the liver. During the last decade, the epidemiology of HCC has been continuously changing in developed countries, due to more effective primary prevention and to successful treatment of virus-related liver diseases. The study aims to examine survival by level of access to care in patients with HCC, for all patients combined and by age. Methods We included 2018 adult patients (15–99 years) diagnosed with a primary liver tumour, registered in the Palermo Province Cancer Registry during 2006–2015, and followed-up to 30 October 2019. We obtained a proxy measure of access to care by linking each re…

AdultMaleCancer Researchmedicine.medical_specialtyCarcinoma HepatocellularAdolescentContext (language use)access to care cancer registries liver cancer public health survival Adolescent AdultAge Distribution Aged Aged 80 and over Carcinoma Hepatocellular Decision Making Organizational Decision Support Techniques Female Follow-Up Studies Health Services Accessibility Health Services Needs and Demand Humans Italy Liver Neoplasms Male Middle Aged Registries Survival Analysis Young AdultSettore MED/42 - Igiene Generale E Applicatalcsh:RC254-282survivalHealth Services AccessibilityDecision Support Techniquesliver cancerYoung AdultAge DistributionInternal medicineHealth careEpidemiologyGeneticsmedicineHumansRegistriesDecision Making OrganizationalAgedAged 80 and overaccess to careHealth Services Needs and Demandbusiness.industryPublic healthLiver Neoplasmspublic healthMiddle Agedlcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogensmedicine.diseaseSurvival AnalysisCancer registryItalyOncologycancer registriesHepatocellular carcinomaAmbulatoryFemaleLiver cancerbusinessFollow-Up StudiesResearch ArticleBMC Cancer
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Cytochrome P450 2E1 variable number tandem repeat polymorphisms and health risks: A genotype-phenotype study in cancers associated with drinking and/…

2012

Cytochrome P450 2E1 (CYP2E1) is one of the main enzymes involved in the oxidation of ethanol and in the transformation of a number of potentially dangerous compounds. It has various polymorphic sites, one of which is a variable number tandem repeat (VNTR) polymorphism previously described in the 5'-flanking region. The aim of this study was to investigate the genotype-phenotype association between CYP2E1 VNTR polymorphisms and risky health habits in healthy subjects and to analyze the associations between these polymorphisms with drinking- and/or smoking-related cancers. We analyzed 166 healthy subjects by genotyping for the CYP2E1 VNTR polymorphism associated with drinking and/or smoking h…

AdultMaleCancer Researchmedicine.medical_specialtyCarcinoma HepatocellularAlcohol Drinkinghuman genetic variability genetic factors cytochrome P450 2E1 variable number tandem repeat polymorphisms predis-posing alleles health risks drinking- and/or smoking-related cancer.Minisatellite RepeatsBiologyBiochemistryGastroenterologyRestriction fragmentYoung AdultRisk-TakingRisk FactorsInternal medicineGenotypeOdds RatioGeneticsmedicineHumansGenetic Predisposition to DiseaseMolecular BiologyGenotypingGenetic Association StudiesGeneticsPolymorphism GeneticLiver NeoplasmsSmokingCytochrome P-450 CYP2E1Odds ratiomedicine.diseaseConfidence intervalPancreatic NeoplasmsVariable number tandem repeatSettore BIO/18 - GeneticaOncologyCase-Control StudiesHepatocellular carcinomabiology.proteinMolecular MedicineAdenocarcinomaFemalePolymorphism Restriction Fragment Length
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Downregulation of organic cation transporters OCT1 (SLC22A1) and OCT3 (SLC22A3) in human hepatocellular carcinoma and their prognostic significance

2012

Abstract Background Organic cation transporters (OCT) are responsible for the uptake and intracellular inactivation of a broad spectrum of endogenous substrates and detoxification of xenobiotics and chemotherapeutics. The transporters became pharmaceutically interesting, because OCTs are determinants of the cytotoxicity of platin derivates and the transport activity has been shown to correlate with the sensitivity of tumors towards tyrosine kinase inhibitors. No data exist about the relevance of OCTs in hepatocellular carcinoma (HCC). Methods OCT1 (SLC22A1) and OCT3 (SLC22A3) mRNA expression was measured in primary human HCC and corresponding non neoplastic tumor surrounding tissue (TST) by…

AdultMaleCancer Researchmedicine.medical_specialtyCarcinoma HepatocellularOrganic Cation Transport ProteinsHepatocellular carcinomaBlotting WesternDown-RegulationOCT3Real-Time Polymerase Chain ReactionOCT1lcsh:RC254-282SLC22A3Downregulation and upregulationInternal medicineGeneticsmedicineHumansRNA MessengerHCCTyrosineSLC22A3CytotoxicitySLC22A1AgedAged 80 and overOrganic cation transport proteinsbiologybusiness.industryLiver NeoplasmsOrganic Cation Transporter 1TransporterMiddle AgedPrognosislcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogensmedicine.diseaseSurvival AnalysisNeoplasm ProteinsEndocrinologyOncologyHepatocellular carcinomaCancer researchbiology.proteinFemalebusinessTyrosine kinaseResearch ArticleBMC Cancer
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Structure, chromosomal localization, and brain expression of human Cx36 gene

1999

Rat connexin-36 (Cx36) is the first gap junction protein shown to be expressed predominantly in neuronal cells of the mammalian central nervous system. As a prerequisite for studies devoted to the investigation of the possible role of this connexin in human neurological diseases, we report the cloning and sequencing of the human Cx36 gene, its chromosomal localization, and its pattern of expression in the human brain analyzed by radioactive in situ hybridization. The determination of the human gene sequence revealed that the coding sequence of Cx36 is highly conserved (98% identity at the protein level with the mouse and rat Cx36 and 80% with the ortholog perch and skate Cx35), and that the…

AdultMaleCandidate geneAdolescentgenetic structuresMolecular Sequence DataIn situ hybridizationBiologyHippocampal formationPolymerase Chain ReactionConnexinsMiceCellular and Molecular NeurosciencemedicineAnimalsHumansCoding regionAmino Acid SequenceSkates FishCloning MolecularEye ProteinsPeptide Chain Initiation TranslationalGeneIn Situ Hybridization FluorescenceChromosomes Human Pair 15Genomic LibrarySequence Homology Amino Acidmedicine.diagnostic_testBrainChromosome MappingHuman brainMiddle AgedMolecular biologyIntronsRatsmedicine.anatomical_structureSpinal CordOrgan SpecificityPerchesCerebellar cortexFemalesense organsSequence AlignmentFluorescence in situ hybridizationJournal of Neuroscience Research
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MicroRNA hsa-miR-4717-5p regulates RGS2 and may be a risk factor for anxiety-related traits

2015

Regulator of G-protein Signaling 2 (RGS2) is a key regulator of G-protein-coupled signaling pathways involved in fear and anxiety. Data from rodent models and genetic analysis of anxiety-related traits and disorders in humans suggest down-regulation of RGS2 expression to be a risk factor for anxiety. Here we investigated, whether genetic variation in microRNAs mediating posttranscriptional down-regulation of RGS2 may be a risk factor for anxiety as well. 75 microRNAs predicted to regulate RGS2 were identified by four bioinformatic algorithms and validated experimentally by luciferase reporter gene assays. Specificity was confirmed for six microRNAs (hsa-miR-1271-5p, hsa-miR-22-3p, hsa-miR-3…

AdultMaleCandidate geneSingle-nucleotide polymorphismMIR4717ComorbidityBiologyBioinformaticsPolymorphism Single NucleotideCellular and Molecular NeuroscienceGenes ReporterRisk FactorsmedicineHumansIKBKEGenetic Predisposition to DiseaseAllelepanic disorderLuciferases3' Untranslated RegionsAgoraphobiaAllelesGenetic Association StudiesGenetics (clinical)miRNAGeneticsPanic disorderassociationComputational BiologyReproducibility of Resultsmedicine.diseaseAnxiety DisordersMicroRNAsPsychiatry and Mental healthGene Expression RegulationCase-Control StudiesLinear ModelsAnxiety sensitivityAnxietyFemalemedicine.symptomgene regulationRGS ProteinsAgoraphobiaAmerican Journal of Medical Genetics Part B-neuropsychiatric Genetics
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HBV virological suppression: still not enough to save from hepatocellular carcinoma. A case report on a 15-year, real-life story

2017

Among HIV-infected patients worldwide, 2-4 million are chronically infected with HBV. We report a 15-year, real-life story of a patient with HBV-HIV coinfection, who developed HCC despite high treatment adherence and complete viral suppression. The aim of our report is to alert the infectious diseases community to monitor the possible development of HCC regardless of high treatment adherence and complete viral suppression.

AdultMaleCarcinoma HepatocellularCoinfectionMedicine (all)Liver NeoplasmsHIVVirological suppressionHIV InfectionsAntiviral AgentsMedication AdherenceHepatitis B ChronicHBVHumansHCCRecenti progressi in medicina
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