Search results for "CIDI"

Inflamed adult pharynx tissues and swimming larva of Ciona intestinalis share CiTNFalpha-producing cells.

2010

In situ hybridisation and immunohistochemistry analyses have shown that the Ciona intestinalis tumour necrosis factor alpha gene (CiTNFalpha), which has been previously cloned and sequenced, is expressed either during the inflammatory pharynx response to lipopolysaccharide (LPS) or during the swimming larval phase of development. Granulocytes with large granules and compartment/morula cells are CiTNFalpha-producing cells in both inflamed pharynx and larvae. Pharynx vessel endothelium also takes part in the inflammatory response. Haemocyte nodules in the vessel lumen or associated with the endothelium suggest the involvement of CiTNFalpha in recruiting lymphocyte-like cells and promoting the…

Ciona intestinalis galectin (CiLgals-a and CiLgals-b) genes are differentially expressed in endostyle zones and challenged by LPS

2015

Abstract Immunohistochemical and in situ hybridization assays were performed to answer the question whether the endostyle, that is the initial gastro-intestinal trait of Ciona intestinalis pharynx, is involved in galectin (CiLgals-a and CiLgals-b) production during the pharynx inflammatory response to LPS inoculation. Specific anti-CiLgal-a and anti-CiLgals-b antibodies, and oligonucleotide probes, that mark inflammatory hemocytes inside the pharynx vessels and vessel epithelium as shown by a previous paper, were assayed on endostyle histological sections. For the first time, we show that galectins are produced by endostyle zones, and both CiLgals-a and –b genes are upregulated by LPS. CiLg…

CiC3-1a-Mediated Chemotaxis in the Deuterostome Invertebrate Ciona intestinalis (Urochordata)

2003

Abstract Deuterostome invertebrates possess complement genes, and in limited instances complement-mediated functions have been reported in these organisms. However, the organization of the complement pathway(s), as well as the functions exerted by the cloned gene products, are largely unknown. To address the issue of the presence of an inflammatory pathway in ascidians, we expressed in Escherichia coli the fragment of Ciona intestinalis C3-1 corresponding to mammalian complement C3a (rCiC3-1a) and assessed its chemotactic activity on C. intestinalis hemocytes. We found that the migration of C. intestinalis hemocytes toward rCiC3-1a was dose dependent, peaking at 500 nM, and was specific for…

Malas madres. De brujas voraces a fantasmas letales

2019

El miedo a las mujeres se expresó desde antiguo en la figura de la madre insensible y cruel, capaz de arrebatar la vida a sus hijos. Desde la Medea griega, el mito de la infanticida terminó plasmándose en la Europa Moderna en las brujas devoradoras de niños. Entre los arquetipos extremos de la madre-bruja asesina y la madre idealizada, se sitúa un tipo de maldad ambigua y sutil: la de la madre dominante que, aunque no elimina a sus hijos físicamente, ejerce una influencia debilitadora y maligna sobre ellos. El tabú de las malas madres afloró tímidamente en la literatura europea desde la Edad Media, para alcanzar su expresión más refinada con el auge de la narrativa gótica, la Ilustración y …

385 MOLECULAR STAGES OF PDGFB DRIVEN LIVER FIBROSIS: LESONS FROM A TRANSGENIC MOUSE MODEL

2012

underlying pathomechanisms is hampered by the lack of a suitable animal model. To circumvent this problem, we studied hepcidinknockout (KO) mice as a model of iron-overload associated liver disease. Methods: 6 and 12 month-old hepcidin-KO and -wild type (WT) mice fed 3% iron carbonyl-containing diet (Fe-diet) since four weeks of age were compared to age-matched WT and KO animals kept on standard diet. The liver phenotype was quantified serologically as well as morphometrically based on hematoxylin & eosin, Prussian blue and Sirius red stainings. Liver iron content was determined by atomic mass absorption. Liver fibrosis development was determined by collagen RT-PCR and hydroxyproline assay.…

La sophrosyne dei Greci. Due saggi di L. Gernet a confronto : « La préhistoire d’une vertu morale : la “tempérance” chez les Grecs » (in Les Grecs sa…

2021

Il contributo propone la rilettura dei seguenti due saggi dello studioso francese Louis Gernet sul tema della sophrosyne: « La préhistoire d’une vertu morale : la “tempérance” chez les Grecs » (in Les Grecs sans miracle, a cura di R. Di Donato, Parigi, 1983, 48-57) e « Thucydide et l’histoire » (in Annales. Histoire, Sciences Sociales, 20/3 [1965], 570-575. The paper proposes a re-reading of the following two essays by the French scholar Louis Gernet on the theme of sophrosyne: "La préhistoire d'une vertu morale : la "tempérance" chez les Grecs" (in Les Grecs sans miracle, edited by R. Di Donato, Paris, 1983, 48-57) and "Thucydides et l'histoire" (Annales et l'histoire) and "Thucydides et l…

Structural characterization of the α-hemolysin monomer fromStaphylococcus aureus

2008

α-Hemolysin from Staphylococcus aureus is secreted as a water-soluble monomer and assembles on membranes to oligomerize into a homo-heptameric, water-filled pore. These pores lead to lysis and cell death. Although the structure of the heptameric pore is solved by means of X-ray crystallography, structures of intermediate states—from the soluble monomer to all potential “pre-pore” structures—are yet unknown. Here, we propose a model of the monomeric α-hemolysin in solution based on molecular modeling, verified by small angle X-ray scattering data. This structure reveals details of the monomeric conformation of the α-hemolysin, for example inherent flexibility, along with definite differences…

SUICIDI D'ONORE

2013

Circumdatin H, a new inhibitor of mitochondrial NADH oxidase, from Aspergillus ochraceus

2005

Circumdatin H (1), a new alkaloid from the culture broth of Aspergillus ochraceus, has been isolated, together with a known circumdatin, circumdatin E (2) and other known compounds: flavacol (3) and stephacidin A (4). The structure of 1 was established on the basis of chemical and spectral evidence. All of these alkaloids showed biological activity as inhibitors of the mammalian mitochondrial respiratory chain.

Ocean acidification affects fish spawning but not paternity at CO2 seeps

2016

Fish exhibit impaired sensory function and altered behaviour at levels of ocean acidification expected to occur owing to anthropogenic carbon dioxide emissions during this century. We provide the first evidence of the effects of ocean acidification on reproductive behaviour of fish in the wild. Satellite and sneaker male ocellated wrasse ( Symphodus ocellatus ) compete to fertilize eggs guarded by dominant nesting males. Key mating behaviours such as dominant male courtship and nest defence did not differ between sites with ambient versus elevated CO 2 concentrations. Dominant males did, however, experience significantly lower rates of pair spawning at elevated CO 2 levels. Despite the hig…