Search results for "CKD"

Evidence of oxidative stress in very long chain fatty acid--treated oligodendrocytes and potentialization of ROS production using RNA interference-di…

2011

X-linked adrenoleukodystrophy (X-ALD) and pseudo neonatal adrenoleukodystrophy (P-NALD) are neurodegenerative demyelinating diseases resulting from the functional loss of the peroxisomal ATP-binding cassette transporter D (ABCD1) and from single peroxisomal enzyme deficiency (Acyl-CoA oxidase1: ACOX1), respectively. As these proteins are involved in the catabolism of very long chain fatty acids (VLCFA: C24:0, C26:0), X-ALD and P-NALD patients are characterized by the accumulation of VLCFA in plasma and tissues. Since peroxisomes are involved in the metabolism of reactive oxygen species (ROS) and nitrogen species (RNS), we examined the impact of VLCFA on the oxidative status of 158N murine o…

2021

Myoglobin (MB) is an oxygen-binding protein usually found in cardiac myocytes and skeletal muscle fibers. It may function as a temporary storage and transport protein for O2 but could also have scavenging capacity for reactive oxygen and nitrogen species. In addition, MB has recently been identified as a hallmark in luminal breast cancer and was shown to be robustly induced under hypoxia. Cellular responses to hypoxia are regulated by the transcription factor hypoxia-inducible factor (HIF). For exploring the function of MB in breast cancer, we employed the human cell line MDA-MB-468. Cells were grown in monolayer or as 3D multicellular spheroids, which mimic the in vivo avascular tumor arch…

Resveratrol post-transcriptionally regulates pro-inflammatory gene expression via regulation of KSRP RNA binding activity

2014

Resveratrol shows beneficial effects in inflammation-based diseases like cancer, cardiovascular and chronic inflammatory diseases. Therefore, the molecular mechanisms of the anti-inflammatory resveratrol effects deserve more attention. In human epithelial DLD-1 and monocytic Mono Mac 6 cells resveratrol decreased the expression of iNOS, IL-8 and TNF-α by reducing mRNA stability without inhibition of the promoter activity. Shown by pharmacological and siRNA-mediated inhibition, the observed effects are SIRT1-independent. Target-fishing and drug responsive target stability experiments showed selective binding of resveratrol to the RNA-binding protein KSRP, a central post-transcriptional regul…

Inhibition of HSP27 blocks fibrosis development and EMT features by promoting Snail degradation

2013

Idiopathic pulmonary fibrosis (IPF) is a devastating disease characterized by myofibroblast proliferation. Transition of epithelial/mesothelial cells into myofibroblasts [epithelial-to-mesenchymal transition (EMT)] occurs under the influence of transforming growth factor (TGF)-β1, with Snail being a major transcription factor. We study here the role of the heat-shock protein HSP27 in fibrogenesis and EMT. In vitro, we have up- and down-modulated HSP27 expression in mesothelial and epithelial cell lines and studied the expression of different EMT markers induced by TGF-β1. In vivo, we inhibited HSP27 with the antisense oligonucleotide OGX-427 (in phase II clinical trials as anticancer agent)…

Inhibition of TSH/IGF-1 Receptor Crosstalk by Teprotumumab as a Treatment Modality of Thyroid Eye Disease

2021

Abstract Context We previously presented evidence that TSH receptor (TSHR)-stimulating autoantibodies (TSAbs) bind to and activate TSHRs but do not bind to IGF1 receptors (IGF1Rs). Nevertheless, we showed that IGF1Rs were involved in thyroid eye disease (TED) pathogenesis because TSAbs activated crosstalk between TSHR and IGF1R. Teprotumumab, originally generated to inhibit IGF1 binding to IGF1R, was recently approved for the treatment of TED (Tepezza). Objective To investigate the role of TSHR/IGF1R crosstalk in teprotumumab treatment of TED. Design We used orbital fibroblasts from patients with TED (TEDOFs) and measured stimulated hyaluronan (HA) secretion as a measure of orbital fibrobla…

Monitoring Risk Factors and Improving Adherence to Therapy in Patients With Chronic Kidney Disease (Smit-CKD Project): Pilot Observational Study

2022

Background Chronic kidney disease is a major public health issue, with about 13% of the general adult population and 30% of the elderly affected. Patients in the last stage of this disease have an almost uniquely high risk of death and cardiovascular events, with reduced adherence to therapy representing an additional risk factor for cardiovascular morbidity and mortality. Considering the increased penetration of mobile phones, a mobile app could educate patients to autonomously monitor cardiorenal risk factors. Objective With this background in mind, we developed an integrated system of a server and app with the aim of improving self-monitoring of cardiovascular and renal risk factors and…

Interpersonal work resources and school personnel well-being before and after lockdown during the first phase of the COVID-19 pandemic in Finland

2022

This two-wave mixed-methods study used the job demands and resources model to examine the effects of the COVID-19 pandemic on school personnel's work well-being (including burnout, work engagement, and sense of belonging) in spring 2020 in Finland in particular with respect to collegial relationships (respectful engagement) and leadership support. A pre-lockdown survey was administered prior to the pandemic (in January–February, n = 437) and a post-lockdown survey was administered after the two-month lockdown (at the end of May, n = 270). At post-lockdown, the school personnel reported, on average, more exhaustion, less work engagement (measured as enthusiasm and energy at work), and a decr…

Replication Data for: Who can go back to work when the COVID-19 pandemic remits?

2020

The file contains the raw data that was used to make the calculations in the article "Who can go back to work when the COVID-19 pandemic remits?" and that allows to replicate it. Specifically, it contains the following: Corresponding to Social Security in Spain - Affiliates to Social Security in Spain in the month of February 2020, distributed by age and province. - Affiliates by sectors in the different regimes, distributed by provinces. February 2020. - Grouping of sectors according to the National Classification of Economic Activities. Corresponding to the effects of covid-19 - Cases and deaths by age in Spain on April 21, 2020 - Population in Spain by age - Total cases and dea…

Anglicisms and Italian Equivalents in the Era of Covid-19: A Corpus-Based Study of Lockdown

2020

The whole globe has been going through an unprecedented historical moment since World War II. COVID-19, the disease against which we have all been trying to defend ourselves for several months now, has spread worldwide with a significant impact in all spheres of life. With the pandemic outburst and English the language of globalization, several Anglicisms have entered the lexicon of other languages, especially Italian, traditionally open to foreign words. COVID-19-related Anglicisms are used in Italian to such an extent that we can reasonably refer to them as the ‘language of pandemic’. The English words and expressions employed in Italian to refer to the current disease are numerous. Some …

The Effect of the Covid-19 Pandemic on the Epidemiology of Ankle Joint Injuries in Adults and Children

2022

Urazy stawu skokowego ze względu na skalę pro blemu i różne metody zaopatrywania, stanowi istotny problem dla ortopedów. Celem tego badania była ocena wpływu pandemii COVID-19 na epidemiologię i leczenie urazów stawu skokowego. Materiał i metody. W badaniu porównano dane epidemiologiczne dotyczące urazów stawu skokowego u dorosłych i dzieci, zebrane w okresie pandemii COVID-19 (2020) i odpowiadającego jej okresu przed pandemią (2019). Analizie poddano dane epidemiologiczne, demograficzne, metody leczenia, czas oczekiwania na zaopatrzenie operacyjne oraz długość pobytu w szpitalu. Ze względu na brak prac analizujących wpływ pandemii COVID-19 na epidemiologię i metody leczenia urazów stawu sk…