Search results for "CLO"
showing 10 items of 9986 documents
Single amino acid substitutions in the glycoprotein B carboxy terminus influence the fusion from without property of herpes simplex virus type 1.
1995
Syncytial mutations of herpes simplex virus type 1 (HSV-1) strains ANG, ANG path, HFEM, tsB5 and HSZP cause extensive cell fusion and were mapped to the cytoplasmic domain of glycoprotein B (gB), within the syn 3 locus. These strains are so far the only ones which show the phenotype ‘fusion from without’ (FFWO): 60 min after infection with high m.o.i., cells in a tissue culture are fused without transcription and translation of the viral genome. In this report we detected, using the recombinants 27/III and K-7, that an amino acid exchange from Ala to Val at aa position 854 of gB is the main determinant for FFWO activity of strains ANG, ANG path and recombinant K-7. The transfer of this muta…
Characterization of the autoantigen La (SS-B) as a dsRNA unwinding enzyme
1997
During the analysis of the La (SS-B) autoantigen for catalytic activities an ATP-dependent double-stranded RNA unwinding activity was detected. Both native and recombinant La proteins from different species displayed this activity, which could be inhibited by monospecific anti-La antibodies. La protein was able to melt dsRNA substrates with either two 3'-overhangs or a single 3'- and a 5'-overhang. Double-stranded RNAs with two 5'-overhangs were not unwound, indicating that at least one 3'-overhang is required for unwinding. Sequence elements of the La protein that might be involved in dsRNA unwinding, such as an evolutionarily conserved putative ATP-binding motif and an element that is hom…
Cost Efficiency of Hybrid Cloud Storage : Shortening Acquisition Cycle to Mitigate Volume Variation
2015
Hybrid cloud storage infrastructure, which combines cost-effective but inflexible private resources and flexible but premium-priced public cloud storage, allows organizations to operate cost-efficiently under demand volume uncertainty. The extant literature, however, offers a limited analytical insight into the effect that the variation of demand has on the cost-efficient mix of internal and external resources. This paper considers the storage capacity acquisition cycle, i.e. the interval at which the organization re-assesses and acquires additional resources, as a parameter shaping the optimal mix of resources. It introduces a model capturing the compound effect of the acquisition cycle an…
We Shape Our Tools, and Thereafter Our Tools Shape Us
2017
The current and incoming editors in chief present the articles of this current issue of Human Technology. Additionally, they discuss the human component of recent technological macrotrends and the journal’s editorial interests in responding to and exploring them. During Editor in Chief Pertti Hurme’s 3-year term, research reflecting a wide range of topics has been published in Human Technology. This online journal, proudly open-access since its beginning, and will continue to invite innovative and interesting manuscripts for open-access publishing under its new editor in chief, Jukka Jouhki.
Reinforcement of brand relationships in an omnichannel environment: a qualitative study on clothing shopping
2022
Today’s brands operate in an omnichannel environment consisting of different online and offline channels where the brand can be experienced. This qualitative study investigates brand relationship reinforcement in the omnichannel environment among customers of a Finnish clothing brand, R-Collection. The data collection was conducted in 2021 by interviewing ten customers of R-Collection. We examined the brand relationship reinforcement in four channels: brick-and-mortar (B&M), online store, social media, and second hand. The findings show that brand relationships can be rein-forced differently in these channels. In B&M stores, sensing the quality of clothing, a customer experience that meets …
Lagrangian matches between observations from aircraft, lidar and radar in an orographic warm conveyor belt
2020
Warm conveyor belts (WCBs) are important airstreams in extratropical cyclones, often leading to the formation of intense precipitation and the amplification of upper-level ridges. This study presents a case study that involves aircraft, lidar and radar observations in a WCB ascending from western Europe towards the Baltic Sea during the Hydrological Cycle in the Mediterranean Experiment (HyMeX) and T-NAWDEX-Falcon in October 2012, a preparatory campaign for the THORPEX North Atlantic Waveguide and Downstream Impact Experiment (T-NAWDEX). Trajectories were used to link different observations along the WCB, that is, to establish so-called Lagrangian matches between observations. To this aim, …
Application of molecular imprinted polymers for selective solid phase extraction of bisphenol A
2016
Selective molecularly imprinted polymers (MIPs) with bisphenol A as template were synthesized using the non-covalent imprinting approach. MIPs were prepared using thermally initiated polymerization with 1,1’-azobis(cyclohexanecarbonitryle) (ACHN) as initiator and ethylene glycol dimethacrylate (EDMA) as a cross-linking agent. The tested functional monomers included methacrylic acid, acrylamide, and 4-vinylpyridine. The selectivity of the BPA-MIP for the solid phase extraction of bisphenol A was tested in samples containing other related alkylphenols. The polymers prepared in acetonitrile using methacrylic acid or acrylamide as monomer showed the highest selectivity towards target analyte (t…
Dyvalocca : un projet pour l'étude des nuages bas de saison sèche sur la façade atlantique de l'Afrique centrale et de leur impact bioclimatique sur …
2020
Low-level clouds are key components of the climate but are not well represented in weather and climate models. Recently it has been shown that an extensive low-level cloud cover develops during the June-September main dry season in western equatorial Africa, from the coastal plains of Gabon and Congo-Brazzaville to the inland plateaus downstream of the low-mountain crests. Such a cloudy main dry season is unique in the moist Tropics and is likely to explain the presence of the densest and evergreen forests of Central Africa as evapotranspiration is low and the quality of light is high. The ANR-DFG funded “DYVALOCCA” project will conduct from 2020 to 2022 a field campaign, raise existing in-…
Development of an immunochromatographic assay based on carbon nanoparticles for the determination of the phytoregulator forchlorfenuron
2013
Rapid analytical methods enabling the determination of diverse targets are essential in a number of research areas, from clinical diagnostics to feed and food quality and safety. Herein, the development of a quantitative immunochromatographic assay for the detection of the synthetic phytoregulator forchlorfenuron (CPPU) is described. The competitive lateral flow immunoassay (LFIA) was based on the immobilization onto a nitrocellulose membrane of an ovalbumin-CPPU conjugate (test line) and on the use of an immunodetection ligand consisting of carbon nanoparticles labeled with an anti-CPPU monoclonal antibody through interaction with a secondary antibody. The presence of CPPU in horticultural…
Ortho -Functionalized Aryltetrazines by Direct Palladium-Catalyzed C−H Halogenation: Application to Fast Electrophilic Fluorination Reactions
2016
International audience; A general catalyzed direct C-H functionalization of s-tetrazines is reported. Under mild reaction conditions, N-directed ortho-C-H activation of tetrazines allows the introduction of various functional groups, thus forming carbon-heteroatom bonds: C-X (X=I, Br, Cl) and C-O. Based on this methodology, we developed electrophilic mono- and poly-ortho-fluorination of tetrazines. Microwave irradiation was optimized to afford fluorinated s-aryltetrazines, with satisfactory selectivity, within only ten minutes. This work provides an efficient and practical entry for further accessing highly substituted tetrazine derivatives (iodo, bromo, chloro, fluoro, and acetate precurso…