Search results for "CONSTRUCTION"
showing 10 items of 2670 documents
A bicistronic vector backbone for rapid seamless cloning and chimerization of αβT-cell receptor sequences.
2020
To facilitate preclinical testing of T-cell receptors (TCRs) derived from tumor-reactive T-cell clones it is necessary to develop convenient and rapid cloning strategies for the generation of TCR expression constructs. Herein, we describe a pDONR™221 vector backbone allowing to generate Gateway™ compatible entry clones encoding optimized bicistronic αβTCR constructs. It harbors P2A-linked TCR constant regions and head-to-head-oriented recognition sites of the Type IIS restriction enzymes BsmBI and BsaI for seamless cloning of the TCRα and TCRβ V(D)J regions, respectively. Additional well-established TCR optimizations were incorporated to enhance TCR functionality. This included replacing of…
Effect of ageing on the morphology and creep and recovery of polymer-modified bitumens
2018
Polymer additives are used to improve the properties of road bitumens including their oxidative resistance. However, their usage as anti-oxidative materials remains relatively unclear. This study aims to investigate the changes in the morphology and the rheological response of polymer modified bitumens used in road pavement construction caused by ageing. An elastomer (radial styrene butadiene styrene, SBS) and a plastomer (ethyl vinyl acetate, EVA) polymer were mixed with one base bitumen at three polymer concentrations. The bitumens were RTFO and PAV aged. The morphology of the bitumens was captured by fluorescence microscopy while the rheological properties were measured by means of the m…
Morphology, mechanical properties and thermal degradation kinetics of PMMA-zirconia nanocomposites prepared by melt compounding
2012
Zirconia nanoparticles were synthesized by means of a sol-gel method and embedded in poly(methyl methacrylate) (PMMA) by melt compounding. The zirconia was well dispersed in the PMMA matrix, with only a few clusters, especially for the highest investigated zirconia content. NMR results showed heteronuclear dipolar interactions involving the carbons and the surrounding hydrogen nuclei. The effect of the amount of zirconia, in the range of 1–5!wt%, on the thermomechanical properties and thermal degradation kinetics of PMMA was also investigated by means of dynamic mechanical analysis (DMA), thermogravimetric analyses (TGA), and Fourier-transform infrared spectroscopy (FTIR). The presenc…
Motion Analysis for Dynamic 3D Scene Reconstruction and Understanding
2017
This thesis studies the problem of dynamic scene 3D reconstruction and understanding using a calibrated 2D-3D camera setup mounted on a mobile platform via the analysis of objects' motions. For static scenes, the sought 3D map reconstruction can be obtained by registering the point cloud sequence. However, with dynamic scenes, we require a prior step of moving object elimination, which yields to the motion detection and segmentation problems. We provide solutions for the two practical scenarios, namely the known and unknown camera motion cases, respectively. When camera motion is unknown, our 3D-SSC and 3D-SMR algorithms segment the moving objects by analysing their 3D feature trajectories.…
Les couvertures : mener l’eau hors des murs
2017
International audience
L'entretien des couvertures médiévales : quelles approches archéologiques ?
2019
International audience
Introduction : de l’étude archéologique au projet
2016
International audience
Analyses archéométriques des carreaux de l'église de Cudot et de l'abbaye des Écharlis
2011
in : J.-L. Dauphin et E. Chat dir., Le culte de sainte Alpais à Cudot et son renouveau au XIXe siècle, Villeneuve-sur-Yonne
La pierre à bâtir sur les chantiers bourguignons : l'évolution des pratiques du choix du Moyen Âge à l'actuel
2014
International audience
A Hybrid AHP Approach and GIS-Based Methods as Fundamental Tools in the SECAP’s Decision-Making Process
2023
Adapting to climate change and mitigating its impacts are the main challenges for cities today. One objective that the European Commission has set in recent decades is reducing climate change inaction, and several political actions have been implemented. Among these actions, the Covenant of Mayors led to the development and adoption of Sustainable Energy and Climate Action Plans (SECAPs) by many cities worldwide. A challenge that local authorities must face during the development of a SECAP is the identification of the policies to be included in the plan. This paper presents a case study to show the validity of using a hybrid analytic hierarchy process (AHP) approach and various geographic …