Search results for "COV"
showing 10 items of 9749 documents
Molecular Basis of SARS-CoV-2 Nsp1-Induced Immune Translational Shutdown as Revealed by All-Atom Simulations.
2021
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic represents the most severe global health crisis in modern human history. One of the major SARS-CoV-2 virulence factors is nonstructural protein 1 (Nsp1), which, outcompeting with the binding of host mRNA to the human ribosome, triggers a translation shutdown of the host immune system. Here, microsecond-long all-atom simulations of the C-terminal portion of the SARS-CoV-2/SARS-CoV Nsp1 in complex with the 40S ribosome disclose that SARS-CoV-2 Nsp1 has evolved from its SARS-CoV ortholog to more effectively hijack the ribosome by undergoing a critical switch of Q/E158 and E/Q159 residues that perfects Nsp1's interactions…
STAT1 and Its Crucial Role in the Control of Viral Infections
2022
The signal transducer and activator of transcription (STAT) 1 protein plays a key role in the immune response against viruses and other pathogens by transducing, in the nucleus, the signal from type I, type II and type III IFNs. STAT1 activates the transcription of hundreds of genes, some of which have been well characterized for their antiviral properties. STAT1 gene deletion in mice and complete STAT1 deficiency in humans both cause rapid death from severe infections. STAT1 plays a key role in the immunoglobulin class-switch recombination through the upregulation of T-bet; it also plays a key role in the production of T-bet+ memory B cells that contribute to tissue-resident humoral memory…
SARS-CoV-2 envelope protein topology in eukaryotic membranes
2020
Coronavirus E protein is a small membrane protein found in the virus envelope. Different coronavirus E proteins share striking biochemical and functional similarities, but sequence conservation is limited. In this report, we studied the E protein topology from the new SARS-CoV-2 virus both in microsomal membranes and in mammalian cells. Experimental data reveal that E protein is a single-spanning membrane protein with the N-terminus being translocated across the membrane, while the C-terminus is exposed to the cytoplasmic side (Nt lum /Ct cyt ). The defined membrane protein topology of SARS-CoV-2 E protein may provide a useful framework to understand its interaction with other viral and ho…
Tumor targeting of baculovirus displaying a lymphatic homing peptide.
2008
Background Tumor-associated cells and vasculature express attractive molecular markers for site-specific vector targeting. To attain tumor-selective tropism, we recently developed a baculovirus vector displaying the lymphatic homing peptide LyP-1, originally identified by ex vivo/in vivo screening of phage display libraries, on the viral envelope by fusion to the transmembrane anchor of vesicular stomatitis virus G-protein. Methods In the present study, we explored the specificity and kinetics of viral binding and internalization as well as in vivo tumor homing of the LyP-1 displaying virus to elucidate the applicability of baculovirus for targeted therapies. Results We demonstrated that th…
Visual Methodologies and Geography's education in the pandemic time: notes on geopolitics of migration in the "Mediterranean Complex"
2022
<abstract> <p>The relationship between Geography and the Visual has always been strong intertwined. As it is true that Geography has always operates through images (in the form of pictures, creative representations and above all cartographies), in the last two years, with the distance learning due to the spread of the Covid-19 pandemic, this phenomenon has not only increased, but it also became necessary. "The classroom as the most radical space", in the words of bell hooks, had to turn into a virtual space, where images have a fundamental role in the teaching/learning process. This paper wants to analyse the relationship between Geography and the Visual by analysing three image…
L’impatto di Covid-19 sui contratti di locazione commerciale
2021
L’inibizione, durante il lock down, dell’apertura al pubblico di gran parte delle attività economiche e le stringenti misure di contenimento adottate nei mesi successivi hanno reso pressante l’esigenza di ridiscutere condizioni contrattuali (e, in particolare, canoni) pattuiti prima dell’emergenza sanitaria. Il contributo attinge alla disciplina dei vizi del contratto di locazione per offrire una soluzione ai casi nei quali entrambe le parti del contratto rivestono la qualifica di imprenditori commerciali.
Aleksandras Beļcovas portretos atklāto emociju izpēte vizuālajā mākslā 7. klasē
2022
Diplomdarba “Aleksandras Beļcovas portretos atklāto emociju izpēte vizuālajā mākslā 7. klasē” ietvaros autore Monta Meikališa ir pētījusi un analizējusi teorētisko literatūru par Aleksandru Beļcovu un viņas portretos atklātās emocijas, termina “emocijas” izpratni, vecumposma īpatnības, kā arī izglītības standartu un programmas paraugus. Diplomdarba mērķis ir izpētīt Aleksandras Beļcovas portretos atklātās emocijas vizuālās mākslas stundās 7. klasē. Autore izstrādāja un praktiski realizēja uzdevumu kopu vispārizglītojošās skolas 7. klases skolēniem, izvēloties piemērotas mācību metodes, kā arī tika apkopoti un analizēti pedagoģiskajā izmēģinājumā iegūtie rezultāti. Pedagoģiskajā darbībā auto…
Koronan kohtaamat
2020
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University students’ (dis)engagement experiences in synchronous sessions during the COVID-19 pandemic
2022
In the past two years, student engagement in online learning situations has become a mutual concern for educators all over the world. The impact of working in online environments and using video and other communication channels on students’ learning experiences is still not fully understood. The present study addresses this question by drawing on students’ written reflections and interviews from the Finnish higher education context collected during the COVID-19 pandemic. It uses the qualitative method of thematic analysis to investigate students’ experiences of interacting in synchronous sessions and their perceptions on (dis)engagement. The analysis shows the importance of versatile teachi…
Singing is what we do (together) - redefining "together" : A comparative study
2021
This article discusses the impact that the Covid-19 pandemic has had on singing with children in schools by providing a descriptive analysis of a comparative look at children's experiences of their singing habits and emotional well-being during the first Covid-19 lockdown. Covid-19 has established itself as (for now) an ever-present variable in our daily lives. This paper offers a descriptive analysis of a comparative study of the Covid-19 lockdown(s) influence on children's singing habits and emotional well-being in Italy, Austria, and Finland. A survey was carried out on children and young people aged 10-20 from Austria, Finland, and Italy on how the Coronavirus lockdown has impacted thei…