Search results for "CQ"
showing 10 items of 1801 documents
Reproducible and Consistent Quantification of the Saccharomyces cerevisiae Proteome by SWATH-mass spectrometry *
2015
Targeted mass spectrometry by selected reaction monitoring (S/MRM) has proven to be a suitable technique for the consistent and reproducible quantification of proteins across multiple biological samples and a wide dynamic range. This performance profile is an important prerequisite for systems biology and biomedical research. However, the method is limited to the measurements of a few hundred peptides per LC-MS analysis. Recently, we introduced SWATH-MS, a combination of data independent acquisition and targeted data analysis that vastly extends the number of peptides/proteins quantified per sample, while maintaining the favorable performance profile of S/MRM. Here we applied the SWATH-MS t…
Hybrid QconCAT-Based Targeted Absolute and Data-Independent Acquisition-Based Label-Free Quantification Enables In-Depth Proteomic Characterization o…
2021
Wheat amylase/trypsin inhibitors (ATIs) have gained significant relevance as inducers of intestinal and extra-intestinal inflammation. In this study, we present a novel hybrid data-independent acquisition (DIA) liquid chromatography-mass spectrometry (LC-MS) approach, combining QconCAT technology with short microflow LC gradients and DIA and apply the method toward the quantitative proteome analysis of ATI extracts. The presented method is fast, robust, and reproducible and provides precise QconCAT-based absolute quantification of major ATI proteins while simultaneously quantifying the proteome by label-free quantification (LFQ). We analyzed extracts of 60 varieties of common wheat grown in…
In‐depth protein profiling of the postsynaptic density from mouse hippocampus using data‐independent acquisition proteomics
2014
Located at neuronal terminals, the postsynaptic density (PSD) is a highly complex network of cytoskeletal scaffolding and signaling proteins responsible for the transduction and modulation of glutamatergic signaling between neurons. Using ion-mobility enhanced data-independent label-free LC-MS/MS, we established a reference proteome of crude synaptosomes, synaptic junctions, and PSD derived from mouse hippocampus including TOP3-based absolute quantification values for identified proteins. The final dataset across all fractions comprised 49 491 peptides corresponding to 4558 protein groups. Of these, 2102 protein groups were identified in highly purified PSD in at least two biological replic…
"Design and application of a data-independent precursor and product ion repository."
2012
The functional design and application of a data-independent LC-MS precursor and product ion repository for protein identification, quantification, and validation is conceptually described. The ion repository was constructed from the sequence search results of a broad range of discovery experiments investigating various tissue types of two closely related mammalian species. The relative high degree of similarity in protein complement, ion detection, and peptide and protein identification allows for the analysis of normalized precursor and product ion intensity values, as well as standardized retention times, creating a multidimensional/orthogonal queryable, qualitative, and quantitative spac…
The Tense-Aspect system between ontogeny and phylogeny: Evidence from the Proto-Indo-European “Injunctive”
2006
This paper examines early inflectional morphology related to the tense-aspect system of Pre-Indo-European by establishing a correlation between ontogeny and phylogeny in language acquisition and development. It will be argued that historical linguistics can shed light on the long-standing debate over the emergence of tense-aspect morphology. More specifically, the so-called Injunctive forms, which are assumed to be the most ancient verbal inflected items tracing back to the Pre-Indo-European language (see, among others, Lehmann 2002), permit us to infer that the initial grammar of the Proto-language was lacking tense morphology. In other words, the residual category of the Injunctive, which…
Jacqueline Woodson’s narrative style in The Other Side: An African American picture book for children
2012
The Other Side (2001) is a children’s story with multicultural characters and themes that can be regarded as an aesthetic exploration of the human experience in the process of the acquisition of knowledge. Following the Black Arts Movement, Jacqueline Woodson’s work portrays many of the issues that are present in the real world but seldom appear in children’s literature, such as racial division or interracial relationships. Using the metaphor of a fence, this African American author reveals issues of loneliness and friendship, inclusion and exclusion, and the overcoming of prejudice and segregation through the wisdom of Clover and Annie, an African American and a white girl, who become frie…
Acquiescence to opacity
2017
Opacity may affect both the means used to implement policies and the real objectives that they pursue. Our concern with opacity is limited to the cases when it is the result of obfuscation. that is, of some effort on the part of governments or other public bodies (central banks or international organisations) to hide or misrepresent their choices. In the literature concerned with accounting for inefficient policies, there are now models in which opacity plays no significant role. This chapter provides a number of mechanisms that account for or lead to the phenomenon the authors are interested in, that is, voters preferring a policy to be opaque rather than transparent. It then discusses two…
Aetiology of community acquired pneumonia in Valencia, Spain: a multicentre prospective study.
1991
A year long multicentre prospective study was carried out in the Valencia region of Spain, to determine the cause of community acquired pneumonia. The study was based on 510 of 833 patients with pneumonia. Of these, 462 were admitted to hospital, where 31 patients died. A cause was established in only 281 cases--208 of bacterial, 60 of viral, and 13 of mixed infection. The most common microorganisms were Streptococcus pneumoniae (14.5%), Legionella sp (14%), Influenza virus (8%), and Mycoplasma pneumoniae (4%). There was a higher incidence of Legionella sp than in other studies.
Pulmonary aspergilloma in a patient with AIDS.
1995
Aspergillus infections are rare in the course of AIDS. They mostly occur as invasive destructive disease in patients with severe CD4 cell depletion. An unusual case of a homosexual AIDS patient who developed a pulmonary aspergilloma is presented.
Community-Acquired Pneumonia Patients at Risk for Early and Long-term Cardiovascular Events Are Identified by Cardiac Biomarkers.
2019
Background Community-acquired pneumonia (CAP) increases the risk of cardiovascular complications during and following the episode. The goal of this study was to determine the usefulness of cardiovascular and inflammatory biomarkers for assessing the risk of early (within 30 days) or long-term (1-year follow-up) cardiovascular events. Methods A total of 730 hospitalized patients with CAP were prospectively followed up during 1 year. Cardiovascular (proadrenomedullin [proADM], pro-B-type natriuretic peptide (proBNP), proendothelin-1, and troponin T) and inflammatory (interleukin 6 [IL-6], C-reactive protein, and procalcitonin) biomarkers were measured on day 1, at day 4/5, and at day 30. Resu…