Search results for "CRIS"
showing 10 items of 2250 documents
Nucleotide excision repair of abasic DNA lesions
2019
AbstractApurinic/apyrimidinic (AP) sites are a class of highly mutagenic and toxic DNA lesions arising in the genome from a number of exogenous and endogenous sources. Repair of AP lesions takes place predominantly by the base excision pathway (BER). However, among chemically heterogeneous AP lesions formed in DNA, some are resistant to the endonuclease APE1 and thus refractory to BER. Here, we employed two types of reporter constructs accommodating synthetic APE1-resistant AP lesions to investigate the auxiliary repair mechanisms in human cells. By combined analyses of recovery of the transcription rate and suppression of transcriptional mutagenesis at specifically positioned AP lesions, w…
Improvement of baculovirus as protein expression vector and as biopesticide by CRISPR/Cas9 editing
2019
The clustered regularly interspaced short palindromic repeats (CRISPR) system?associated Cas9 endonuclease is a molecular tool that enables specific sequence editing with high efficiency. In this study, we have explored the use of CRISPR/Cas9 system for the engineering of baculovirus. We have shown that the delivering of Cas9-single guide RNA ribonucleoprotein (RNP) complex with or without DNA repair template into Sf21 insect cells through lipofection might be efficient to produce knockouts as well as knock-ins into the baculovirus. To evaluate potential application of our CRISPR/Cas9 method to improve baculovirus as protein expression vector and as biopesticide, we attempted to knockout se…
Las repercusiones de la crisis triguera de 1629-1631 sobre las finanzas de la ciudad de Valencia
2005
La incidencia de la crisis triguera de 1629-1631 obligó a la ciudad de Valencia a desarrollar diversos sistemas de avituallamiento. Pero ello no pudo impedir que se produjera un excepcional aumento de los precios del cereal, frente al cual el Municipio desarrolló una arriesgada política que abocó en la falta de numerario de la Taula de Canvis. La búsqueda de soluciones ante el déficit se tradujo en la elaboración, desde diferentes instancias políticas, de numerosos memoriales proponiendo múltiples y variados arbitrios frente a la crisis, cuyo análisis constituye el objetivo primordial de este artículo. The effect of the wheat crisis of 1629-1631 forced the city of Valencia to develop variou…
Social Media Monitoring for Crisis Communication: Process, Methods and Trends in the Scientific Literature
2014
This literature review study aims at clarifying current knowledge on social media monitoring from the perspective of organizational communication and public relations. It also contributes to crisis communication by shedding light on how fast developing social media discourse can be followed and analysed in order to understand citizens’ needs throughout all the phases of a crisis. The findings of this study reveal a number of insights in the scientific literature on the concept of monitoring, the monitoring process, methods, tools and solutions, methodological issues and trends covering the years 2009–2012. In the literature, social media monitoring is described as a process which comprises …
Una aproximación a la situación de los Agentes de Desarrollo Local (ADL) en el proceso de creación de nuevos polígonos industriales
2008
Un modelo de desarrollo local como el que tenemos implantado, aparecido a mediados de los años ochenta como respuesta a una situación de grave crisis económica y social, presenta hoy en día grandes carencias de tipo estructural, muchas de las cuales se han ido originando de facto con el transcurso de los años sin que nadie haya recogido el testigo de completar dichos vacíos. En esta nota destacamos una de ellas que consideramos muy visible en la actualidad: ante la floración continua de polígonos industriales –fuente teórica de empleo a nivel local– denotamos como el instrumento básico creado por el modelo de desarrollo local, los Agentes de Empleo y Desarrollo Local –AEDL o simplemente ADL…
¿La ONU, irrecuperable? / 2
2005
Fire simulation-based adaptation of SmartRescue App for serious game: Design, setup and user experience
2015
Managing the crisis by embracing game and simulation elements and human participation into an interactive system is a mean to learn about responding to unexpected events. This so-called serious game approach is adopted in a summer school for crisis management attended by doctoral students and practitioners, as a part of its learning curriculum. The participants took part in the Disaster in my Backyard serious game, designed as a realistic crisis environment. A smartphone app encompassing fire simulations of a five-story apartment, showing how the flame, smoke and temperature of the fire developed over time from floor to floor, was tested in this serious game scenario. The color-coding of sm…
La crisis gremial y los problemas de la sedería valenciana (finales del siglo XVIII y principios del siglo XIX)
1992
La reforma del sistema gremial alcanza una de sus medidas más radicales con la abolición de los gremios de torcedores de seda en 1793. En estas páginas se analiza la medida abolicionista en el marco general de los problemas de la sedería valenciana. En la misma, la hilatura, de la que el torcido es su fase superior y última, había llegado a constituirse en un cuello de botella que comprometía su futuro. En los años aquí contemplados, se proponen y ensayan diversas alternativas que solucionen las disfunciones que generaba la peculiar división del trabajo que obraba en la sedería. La no implicación del capital comercial de la ciudad en la necesaria reconversión de la hilatura será un factor c…
La diversidad de proyectos políticos en el primer debate preconstitucional español: Canga Argüelles, Ribelles y Borrul en el contexto de la política …
2002
The decree by which the Cortes of 22 May 1809 were summoned by the Junta Suprema of the realm marked the beginning of a political debate of great intensity in Spain. Through this debate the different political positions and the various ideological profiles arising from the deep constitutional crisis of 1808 became gradually crystallised. This article approaches that debate from the regional perspective of one of the territories or provinces of the Bourbon monarchy—namely, the old Kingdom of Valencia— and by examining three figures relatively unknown to historians, José Canga Argüelles, Bartolomé Ribelles and Francisco Xavier Borrul. Through an analysis of their works we can identify the div…