Search results for "CULTURES"

A new culturing strategy optimises Drosophila primary cell cultures for structural and functional analyses

2004

Abstract Neurons in primary cell cultures provide important experimental possibilities complementing or substituting those in the nervous system. However, Drosophila primary cell cultures have unfortunate limitations: they lack either a range of naturally occurring cell types, or of mature physiological properties. Here, we demonstrate a strategy which supports both aspects integrated in one culture: Initial culturing in conventional serum-supplemented Schneider's medium (SM 20K ) guarantees acquisition of all properties known from 30 years of work on cell type-specific differentiation in this medium. Through subsequent shift to newly developed active Schneider's medium (SM active ), neuron…

Designing trehalose-conjugated peptides for the inhibition of Alzheimer’s Aβ oligomerization and neurotoxicity

2008

La fin du Néolithique dans le sud-est de la France. Concepts techniques, culturels et chronologiques de 1954 à 2004

2006

The mediterranean France final Neolithic, between the end of Middle Neolithic Chasséen (3700-3500 Cal BC) and the end of the transitional period to the early Bronze Age, with the barbed Wire groups (1900-1800 Cal BC), is a complex period marked by the definition of almost about fifteen cultural groups and many of geographical facies and chronological phases. The chronological and technical concepts used are not less complex and varied according to researchers (Recent Neolithic or late Middle Neolithic or Final Neolithic 1, Final Neolithic and Copper Age or only Final Neolithic, and Copper Age-early Bronze Age...). If the Rhone constituted only seldom a real border during the Neolithic, it i…

Campaniformes et cultures locales en France méditerranéenne

2006

Since the 1998 Riva del Garda conference and the synthesis coordinated by J Guilaine (2001), the new bell beakers archaeological data of Mediterranean France make it possible to specify the relations between the Bell Beakers ones and indigenous populations of the final Neolithic in this area. The phase of synchrony between Bell Beakers and local cultures, highlighted for the Early Bell Beakers between 2500 and 2400 before our era, can undoubtedly be wide at the first times, at least, development of the regional groups of recent bell beakers ("Rhodano- Provençal" and "Pyrénéen"). In parallel, differences in relations between these Bell beakers and the indigenous populations can be supposed i…

Territorial and socio-economic organisation in Le Grand-Pressigny

2012

In and around Le Grand-Pressigny (Indre-et-Loire, France), a petrographic study was implementedon 92 ceramic samples from the Final Neolithic sites of Le Petit-Paulmy and Bergeresse (Abilly).Analyses showed that the sediments used were extracted from local sources: Claise and Creusealluvium, local substrate and loessic silts. Quantitatively, the study showed that almost all the ceramicmaterials in Bergeresse and more than half of those in Le Petit-Paulmy come from the valley of theCreuse. The mineralogical compositions of three samples from Le Petit-Paulmy, including one ofunusual form, indicate sources from an exogenous region, the Massif Central (perhaps the alluviumof the Loire or the Al…

La fin des temps néolithiques

2004

The chapter presents the state of knowledge on the end of the Neolithic era in the department of Vaucluse. It evokes the known sites and their distribution, the chronology of the period ranging between the end of the Middle Neolithic and the Early Bronze Age and the archaeological cultures present. Several paragraphs sets of themes take stock of research concerning ceramics, tools, metal, ornament, habitat, economy, burials and art.

Climatic gradients along the windward slopes of Mount Kenya and their implication for crop risks. Part 2 : crop sensitivity.

2016

16 pages; International audience; Mount Kenya is an equatorial mountain whose climatic setting is fairly simple (two rainy seasons in March–May, the Long Rains, and October–December, the Short Rains) though concealing significant spatial variations related to elevation and aspect (part I, Camberlin et al., 2014). This part II is dedicated to the sensitivity of sorghum yields to climate variability in space and time, with a focus on the intra-seasonal characteristics of the rainy seasons. To that aim we use the crop model SARRA-H calibrated for the region and fed with rainfall, temperature, wind speed, humidity and solar radiation data over the period 1973–2001 at three stations located on t…

Neuronal and BBB damage induced by sera from patients with secondary progressive multiple sclerosis.

2009

An important component of the pathogenic process of multiple sclerosis (MS) is the blood-brain barrier (BBB) damage. We recently set an in vitro model of BBB, based on a three-cell-type co-culture system, in which rat neurons and astrocytes synergistically induce brain capillary endothelial cells to form a monolayer with permeability properties resembling those of the physiological BBB. Herein we report that the serum from patients with secondary progressive multiple sclerosis (SPMS) has a damaging effect on isolated neurons. This finding suggests that neuronal damaging in MS could be a primary event and not only secondary to myelin damage, as generally assumed. SPMS serum affects the perme…

Establishment of a pulmonary epithelial barrier on biodegradable poly-L-lactic-acid membranes

2019

Development of biocompatible and functional scaffolds for tissue engineering is a major challenge, especially for development of polarised epithelia that are critical structures in tissue homeostasis. Different in vitro models of the lung epithelial barrier have been characterized using non-degradable polyethylene terephthalate membranes which limits their uses for tissue engineering. Although poly-L-lactic acid (PLLA) membranes are biodegradable, those prepared via conventional Diffusion Induced Phase Separation (DIPS) lack open-porous geometry and show limited permeability compromising their use for epithelial barrier studies. Here we used PLLA membranes prepared via a modification of the…

Early signaling network in tobacco cells elicited with methyl jasmonate and cyclodextrins.

2012

We analyze, for the first time, the early signal transduction pathways triggered by methyl jasmonate (MJ) and cyclodextrins (CDs) in tobacco (Nicotiana tabacum) cell cultures, paying particular attention to changes in cytosolic free Ca(2+) concentration ([Ca(2+)](cyt)), the production of hydrogen peroxide (H(2)O(2)) and nitric oxide (NO), and late events like the induction of capsidiol. Our data indicate that MJ and CDs trigger a [Ca(2+)](cyt) rise promoted by Ca(2+) influx through Ca(2+)-permeable channels. The joint presence of MJ and CDs provokes a first increase in [Ca(2+)](cyt) similar to that observed in MJ-treated cells, followed by a second peak similar to that found in the presence…