Search results for "CULTURES"

Materiali par novadneekeem

1912

Bourdieu y la traducción e interpretación en los servicios públicos: hacia una teoría social

2010

Dentro de los Estudios de Traducción, la Traducción e Interpretación en los Servicios Públicos (TISP) es un área relativamente reciente (Critical Link 1995) en lucha todavía por conseguir el reconocimiento académico y profesional que se merece dentro y fuera de los límites que marca dicha disciplina. Gran parte de las dificultades para conseguir esa aceptación y reconocimiento se halla en la controversia que envuelve el papel del traductor e intérprete en sus actuaciones en los servicios públicos. En un intento por explicar la actuación de estos intérpretes, que con frecuencia va más allá de la invisibilidad y del mero trasvase lingüístico, y siguiendo la influencia que la teoría social de …

Inclusion of Trehalose (TRH) into liposomes to regulate uptake of this cryoprotectant into human hepatocytes.

2009

Purpose. Problems with the limited availability of human hepatocytes for cell transplantation may be overcome by improving the efficiency of their cryopreservation. TRH-loaded liposomes could enhance epatocyte viability by regulating the intracellular uptake of the cryoprotectant [1]. Methods. TRH-loaded liposomes were prepared by film hydration method (EPC, sodium cholate, 200mM TRH solution) [2]. Liposomal size was determined by dynamic light scattering. Intraliposomal TRH content was measured using the Megazyme spectrophotometric method. After incubation (1-5 h, 37°C, 5% CO2, 95% RH) of human hepatocytes with TRH-loaded liposomes or equivalent TRH solution, intracellular sugar content wa…

In vitro evidences of epithelial to mesenchymal transition in low cell-density cultured human fetal hepatocytes

2017

Abstract Culturing fetal hepatocytes in high cell-density allowed stabilization of the hepatocyte phenotype up to 8 weeks, including the maintenance of liver-specific functions. On the other hand, when cultured at low cell-density, fetal hepatocytes underwent morphological modifications and acquired fibroblastic morphology. Since a switch from E-cadherin to vimentin expression accompanied these changes, we hypothesized the occurrence of epithelial-to-mesenchymal transition when fetal hepatocytes were cultured at low cell-density. Changes in gene expressionsuch as up-regulation of fibrosis-related geneswere also observed, suggesting that the low cell-density culture system promoted the acqui…

Expression and developmental regulation of the cystine/glutamate exchanger (xc-) in the rat.

2007

The cystine/glutamate exchanger (antiporter x c − ) is a membrane transporter involved in the uptake of cystine, the rate-limiting amino acid in the synthesis of glutathione. Recent studies suggest that the antiporter plays a role in the slow oxidative excitotoxity and in the pathological effects of β-N-oxalylamino-l-alanine, the molecule responsible for neurolathyrism, a neurotoxic upper motor neuron disease. The mouse cystine/glutamate exchanger has been cloned and showed to be composed of two distinct proteins, one of which being a novel protein, named xCT, of 502 amino acids and 12 putative trans-membrane domains. We have generated and purified a polyclonal antibody to mouse xCT and stu…

Prenatal exposure to the CB1 receptor agonist WIN 55,212-2 causes learning disruption associated with impaired cortical NMDA receptor function and em…

2005

The aim of this study was to investigate whether prenatal exposure to the cannabinoid CB1 receptor agonist WIN 55,212-2 (WIN) at a daily dose devoid of overt signs of toxicity and/or gross malformations (0.5 mg/kg, gestation days 5-20), influences cortical glutamatergic neurotransmission, learning and emotional reactivity in rat offspring. Basal and K+-evoked extracellular glutamate levels were significantly lower in cortical cell cultures obtained from pups exposed to WIN during gestation with respect to those measured in cultures obtained from neonates born from vehicle-treated dams. The addition of NMDA to cortical cell cultures from neonates born from vehicle-treated dams concentration-…

Toxicity of used orthodontic archwires assessed by three-dimensional cell culture

2006

The aim of the present study was to determine whether used orthodontic wires made of different materials cause toxicity and loss of viability on three-dimensional (3D) cell cultures. Three types of orthodontic wires, stainless steel, Nitinol, and TMA (n = 9) which had been used clinically in fixed appliances for a period of 1 month, were retrieved at random from five patients. Both upper and lower archwires were collected and subjected to two different protocols: to assess toxicity, two pieces of each wire were placed on 3D cell cultures (reconstituted human epithelium); to investigate the possibility of cell damage, the 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) ass…

Mediterranean cultures, cities, contemporaneity

2017

L’obiettivo del contributo sui temi legati al Mediterraneo vuole mettere in evidenza come tale spazio abbia perso, nel tempo, il suo ruolo di luogo di incontro, di scambio fra le diverse culture, diventando oggi non più un luogo unitario, ma “un mare trasformato in una delle frontiere più insanguinate della storia” (W. Bauer, 2015). La storia è stata sempre segnata dal movimento di popoli che hanno fatto del Mediterraneo il luogo privilegiato di incontro di tradizioni, religioni e culture differenti, facendo di queste “contaminazioni” una ricchezza da cui attingere, basata sul confronto reciproco. E’ possibile, ancora oggi, considerare il Mediterraneo come uno spazio in cui le diverse cultu…

Social networks : promoting Mexican popular cultures and building a participatory culture

2012

For over 30 years, the General Direction of Popular Cultures has been in charge of promoting, preserving, and disseminating the cultural diversity of Mexico, regarding indigenous and popular cultural practices. With the increasing popularization of Social Networks, it has become almost indispensable to join Social Networks in order to exist digitally, weather you are an individual, an institution or government. Taking this fact in consideration, in 2011 the Direction of Popular Cultures joined the most utilized social Networks in México: Twitter, Facebook and YouTube, as a way to keep users updated about the activities and general information generated by the Direction. Departing form the p…

Skewed Differentiation of Circulating Vγ9Vδ2 T Lymphocytes in Melanoma and Impact on Clinical Outcome

2016

OBJECTIVE:The aim of this study was to evaluate over time circulating γδ T lymphocytes in melanoma patients in terms of frequency, effector functions, and relationship with clinical stage and evolution, by comparing preoperative values to those obtained at a mean follow-up of 36 months or in the event of recurrence or disease progression, and to those of healthy controls. Also, we correlated the presence of tumor-infiltrating γδ T lymphocytes with clinical evolution of melanoma. RESULTS:Mean frequencies of circulating γδ T cells before and after melanoma removal were very similar and comparable to healthy subjects, but patients who progressed to stage III or IV showed a significantly decrea…