Search results for "Cadmi"

Relazione tra autofagia e apoptosi in embrioni di riccio di mare esposti a stress

2012

L’embrione di riccio di mare Paracentrotus lividus è stato utilizzato come organismo modello della biologia dello sviluppo per molti anni ed è considerato il deuterostoma più primitivo, con scheletro calcificato, correlato a protocordati e vertebrati. Tale sistema offre un'eccellente opportunità per studiare le numerose strategie di difesa che gli embrioni mettono in atto contro diverse condizioni di stress. In precedenza, abbiamo riportato che l’esposizione di embrioni a dosi citotossiche di cadmio, provoca l'accumulo intracellulare del metallo e l'attivazione del sistema difensivo, in modo dose-tempo dipendente, attraverso la sintesi di specifiche HSPs e/o l’innesco di apoptosi. Mediante …

Cadmium induces autophagy during development of Paracentrotus lividus embryos.

2010

Effect of cadmium chloride on some mitochondria-related activity and gene expression of human MDA-MB231 breast tumor cells

2008

Expression and localization of AEG-1 in cadmium-treated MDA-MB231 breast cancer cells

2007

Espressione cadmio-dipendente di AEG-1 in cellule tumorali mammarie umane

2006

Cytochemical and molecular analyses on mitochondria of immortalized and neoplastic epithelial cells of the human breast after cadmium treatments

2008

In vivo exposure of the marine sponge Chondrilla nucula Schmidt, 1862 to cadmium (Cd), copper (Cu) and lead (Pb) and its potential use for bioremedia…

2018

Abstract The study aimed to test the cadmium (Cd), copper (Cu) and lead (Pb) bioaccumulation capacity of the marine sponge Chondrilla nucula and to measure the expression of metallothioneins (MTs) by a western blotting assay to explore metal tolerance and its potential use as a bioremediator in contaminated coastal areas. C. nucula was sampled in a protected marine area in order to perform experiments on organisms living in a healthy environment. Under laboratory conditions, the sponges were exposed to increasing concentrations of Cd, Cu and Pb in tanks filled with artificial seawater set up for the experiments. For each metal, three waterborne concentrations (mgL−1) of Cd (0.02–0.04-0.08),…

Polymer aging affects the bioavailability of microplastics-associated contaminants in sea urchin embryos

2022

Microplastics (MPs) in the marine environment undergo complex weathering factors that can affect their ability to interact with different coexisting environmental contaminants (termed here co-contaminants). In this study, the influence of artificially aging using UV on the sorption of a complex mixture of co-contaminants onto MPs was investigated in order to provide meaningful hypotheses on their individual and combined toxicities on sea urchin embryos. A mixture of artificially aged MPs (PS particles and PA microfibers) combined with 2,2′ ,4,4′ - tetrabromodiphenyl ether (BDE-47), or Cd or Cu, both alone and in a mix, were used to expose embryos of Paracentrotus lividus. The effects of pol…

Baseline assessment of heavy metals content and trophic position of the invasive blue swimming crab Portunus segnis (Forskål, 1775) in the Gulf of Ga…

2018

This study provides a baseline assessment of cadmium, copper, iron, manganese, lead, and zinc concentrations in muscles, gills, and exoskeleton of the Lessepsian blue swimming crab Portunus segnis captured in the Gulf of Gabès (Southern Mediterranean Sea, Tunisia) in November 2015. Furthermore, the species’ trophic position is estimated using CN stable isotope analysis. The exoskeleton showed the lowest metal contents; in soft tissues, the essential Cu, Fe, and Zn and the non-essential Cd, Mn, and Pb showed the highest and lowest concentrations, respectively. The crab was characterized by a trophic position of 3.32, confirming its carnivorous trophic habits. Compared with literature data on…

Metallothionein genes in sea urchin embryos and their induction by cadmium

2012

Metallothioneins (MTs) are proteins that play a major role in metal homeostasis and as a reservoir. The MT gene/protein systems of sea urchins are an invaluable model for the study of gene expression regulation and MT isoform-specific functionality of these proteins. We isolated five paralogous MT isogenes and we studied their expression in the sea urchin embryo Paracentrotus lividus. The Cadmium-dependent transcriptional activation of the five isogenes was assessed using quantitative Real Time PCR. Two of the five P. lividus MT (MT-7 and MT-8) isogenes appeared to be constitutively expressed and upregulated upon cadmium treatment. Three isogenes (MT-4/MT-6) are not transcribed in control e…