Search results for "Candida albican"
showing 4 items of 314 documents
Clinical and microbiological diagnosis of oral candidiasis
2013
Introduction: Candidiasis or oral candidiasis is the most frequent mucocutaneous mycosis of the oral cavity. It is produced by the genus Candida, which is found in the oral cavity of 53% of the general population as a common commensal organism. One hundred and fifty species have been isolated in the oral cavity, and 80% of the isolates correspond to Candida albicans, which can colonize the oral cavity alone or in combination with other species. Transformation from commensal organism to pathogen depends on the intervention of different predisposing factors that modify the microenvironment of the oral cavity and favor the appearance of opportunistic infection. The present study offers a liter…
Role of PRRs (TLR2 and Dectin-1) in hematopoietic stem and progenitor cell differentiation: implications in protection against Candida albicans infec…
2020
Detection of infection by hematopoietic stem and progenitor cells (HSPCs) is essential to replace myeloid cells consumed during the immune response. HSPCs express some functional pattern recognition receptors involved in the recognition of Candida albicans. In this context, our group has previously demonstrated that C. albicans yeasts induce proliferation and differentiation of HSPCs via TLR2 and Dectin-1. In the present PhD thesis, we used in vitro and ex vivo models of HSPC differentiation to investigate the functional consequences for mature myeloid cells of exposure of HSPCs to PAMPs or C. albicans yeasts. In vitro experiments show that murine HSPCs continuously exposed to TLR2 or TLR4 …
Aislamiento y caracterización de vesículas extracelulares en especies del género Candida
2017
Se ha desarrollado un método de obtención de exosomas, a partir de cultivos de levaduras del género Candida, que ha permitido obtener microvesículas de elevada homogeneidad y pureza. Mediante microscopía electrónica de transmisión y técnicas de detección de nanopartículas (NTA), se ha podido constatar la presencia de microvesículas de tamaños comprendidos entre 100 y 200 nm. La caracterización de proteínas llevada a cabo por técnicas de Western blot, ha puesto de manifiesto la elevada pureza de los exosomas obtenidos, no existiendo contaminación celular en las preparaciones, tal y como lo indican el resultado positivo frente a calnexina en el lisado celular y negativo en las vesículas extra…
Role of hematopoietic stem and progenitor cells in training immunity against infection
2022
During an infection, haematopoiesis is altered to increase the output of mature myeloid cells to fight off the pathogen. It has been demonstrated that the detection of pattern recognition receptor agonists by hematopoietic stem and progenitor cells (HSPCs) induces their differentiation towards mature myeloid cells with modified phenotypes. In the present PhD thesis, we show that an in vitro transient exposure of HSPCs to live Candida albicans cells is sufficient to induce a trained phenotype of the macrophages they produce in a dectin-1- and TLR2-dependent manner. Additionally, we use an HSPC transplantation mouse model to demonstrate that the direct interaction of β-glucans and their recep…