Search results for "Capsule"
showing 10 items of 223 documents
Controlling the semi-permeability of protein nanocapsules influences the cellular response to macromolecular payloads.
2021
Nanocapsules are an excellent platform for the delivery of macromolecular payloads such as proteins, nucleic acids or polyprodrugs, since they can both protect the sensitive cargo and target its delivery to the desired site of action. However, the release of macromolecules from nanocapsules remains a challenge due to their restricted diffusion through the nanoshell compared to small molecule cargo. Here, we designed degradable protein nanocapsules with varying crosslinking densities of the nanoshell to control the release of model macromolecules. While the crosslinking did not influence the degradability of the capsules by natural proteases, it significantly affected the release profiles. F…
Studentu karcera laika kapsulas
2023
Raksts veltīts unikālam atklājumam Latvijas Universitātes vēsturiskajā Studentu karcerī, kas glabājies kopš 19./20. gs. mijas. Tiek analizētas 3 papirosu paciņas un 1 sērkociņu kastīte no Krievijas impērijas laika, noskaidrojot tā izcelsmi, pielietojumu un zinātnisko novitāti. Atrastie priekšmeti liecina, ka Studentu karcerī ieslēgtie studenti spēja apiet Rīgas Politehnikuma Studentu disciplinēšanas regulā noteikto sodu par smēķēšanu. Priekšmeti arī ir Latvijas industriālās vēstures atstāts mantojums, kas liecina par Baltijas reģiona nozīmi Krievijas impērijas rūpniecības attīstībā.
First report of Antigastra catalaunalis on sesame in Greece.
2017
Τον Αύγουστο του 2016, καταγράφηκαν σοβαρές προσβολές σε καλλιέργειες σουσαμιού από το έντομο Antigastra catalaunalis (Duponchel) (Lepidoptera: Crambidae) σε αγροτικές περιοχές της Περιφερειακής Ενότητας Δράμας. Το A. catalaunalis είναι ένας σημαντικός εχθρός της καλλιέργειας του σουσαμιού σε περιοχές όπου αυτό καλλιεργείται. Παρατηρήθηκαν συμπτώματα προσβολής από τις προνύμφες του εντόμου σε φύλλα, βλαστούς και κάψες. Οι προσβολές καταγράφηκαν σε όλους τους αγρούς με καλλιέργεια σουσαμιού που επιθεωρήθηκαν, με επίπεδα προσβολής υψηλά τα οποία προκάλεσαν οικονομική απώλεια. Στο βαθμό που γνωρίζουμε, αυτή είναι η πρώτη αναφορά σοβαρών προσβολών σουσαμιού από το Α. catalaunalis στην Ελλάδα.
Observations on a new species of Unicapsula (Myxosporida) in the fish Maena smaris (L.) by conventional and electronmicroscopy
1975
A myxosporidian parasite believed to be congeneric with Unicapsula muscularis Davis (1924), type (and only) species of this genus, was found in the dorsal muscles of the fish Maena smaris (L.) at Banyuls-sur-Mer, France. The spores were tripartite, with corresponding components of the three parts differentially developed. One part consisted of a small shell valve overlying no more than a trace of sporoplasm but covering a polar apparatus with well-developed polar capsule. Each of the other two parts consisted of a long shell valve covering a large sporoplasm and a polar apparatus with only the rudiment of a polar capsule. Electron microscope pictures of the trophozoite showed evidence of pi…
Bioresponsive poly(amidoamine)s designed for intracellular protein delivery.
2013
Poly(amidoamine)s with bioreducible disulfide linkages in the main chain (SS-PAAs) and pH-responsive, negatively charged citraconate groups in the sidechain have been designed for effective intracellular delivery and release of proteins with a net positive charge at neutral pH. Using lysozyme as a cationic model protein these water soluble polymers efficiently self-assemble into nanocomplexes by charge attraction. At pH 5 (the endosomal pH) the amide linkages connecting the citraconate groups in the sidechains of the SS-PAAs are hydrolyzed by intramolecular catalysis, resulting in expulsion of the negative citraconate groups and formation of protonated amine groups, resulting in charge reve…
Inclusion complexes of Cethyl-2-methylresorcinarene and pyridine N-oxides: breaking the C–I⋯−O–N+ halogen bond by host–guest complexation
2016
C ethyl-2-Methylresorcinarene forms host–guest complexes with aromatic N-oxides through multiple intra- and intermolecular hydrogen bonds and C–H⋯π interactions. The host shows conformational flexibility to accommodate 3-methylpyridine N-oxide, while retaining a crown conformation for 2-methyl- and 4-methoxypyridine N-oxides highlighting the substituent effect of the guest. N-Methylmorpholine N-oxide, a 6-membered ring aliphatic N-oxide with a methyl at the N-oxide nitrogen, is bound by the equatorial −N–CH3 group located deep in the cavity. 2-Iodopyridine N-oxide is the only guest that manifests intermolecular N–O⋯I–C halogen bond interactions, which are broken down by the host resulting i…
Cryopreservation of Digitalis obscura selected genotypes by encapsulation-dehydration
2001
Shoot-tips from several genotypes of the cardenolide-producing perennial shrub Digitalis obscura L. were successfully cryopreserved using the encapsulation-dehydration technique. Precultivation on MS medium containing 0.5 M sucrose, followed by 2.5 h dehydration (final weight 30 %) induced shoot regrowth in 42 % of cryopreserved shoot-tips. Cold-hardening of the in vitro cultures before sucrose treatment dramatically increased shoot recovery up to 86 %. The optimized cryopreservation protocol was then employed using different shoot cultures from five D. obscura genotypes. Responses to cryopreservation depended mainly on the genotype, best results being obtained when shoot tips from HU3 and …
Expression of MAP1a and MAP1b in the ganglionic eminence and the internal capsule of the human fetal brain.
2001
The expression of microtubule-associated proteins 1a and 1b (MAP1a and 1b) were investigated in two transient structures, the ganglionic eminence (GE) being a prominent part of the telencephalic proliferative zone and the perireticular nucleus (PR) within the internal capsule (IC). Anti-MAP1a immunolabels PR neurons from 18 weeks of gestation (wg) onwards, whereas anti-MAP1b immunolabels long IC fibers between 18 and 22 wg. MAP1b is further present in thalamic fibers that seem to terminate at the medial margin of the GE, in a moderate number of cells of the GE and its medial extension, the gangliothalamic body (GTB). From 26 to 33 wg MAP1b is expressed in short fiber bundles of the IC, a fe…
Ferricytochrome c encapsulated in silica hydrogels: correlation between active site dynamics and solvent structure.
2003
Ferricytochrome c encapsulated in silica hydrogels has been prepared by the sol-gel technique following, with some modifications, the procedure originally developed by Ellerby et al. (Science 255 1113 (1992)). A suitable preparation of hydrogels enables having both 'wet' and 'dry' samples. Wet samples have a high water content: as the temperature is lowered below approximately 260 K, water freezes and the samples crack. On the contrary, dry samples have a low water content (hydration h approximately equal 0.35): in these conditions water does not freeze even at cryogenic temperatures and the samples remain transparent and non-cracking. The dynamics of ferricytochrome c and its dependence on…
Effects of salinity and temperature on long-term survival of the eel pathogen Vibrio vulnificus biotype 2 (serovar E)
1999
ABSTRACT Vibrio vulnificus biotype 2 (serovar E) is a primary eel pathogen. In this study, we performed long-term survival experiments to investigate whether the aquatic ecosystem can be a reservoir for this bacterium. We have used microcosms containing water of different salinities (ranging from 0.3 to 3.8%) maintained at three temperatures (12, 25, and 30°C). Temperature and salinity significantly affected long-term survival: (i) the optimal salinity for survival was 1.5%; (ii) lower salinities reduced survival, although they were nonlethal; and (ii) the optimal temperature for survival was dependent on the salinity (25°C for microcosms at 0.3 and 0.5% and 12°C for microcosms at 1.5 to 3.…