Search results for "Carboni"
showing 10 items of 357 documents
A GIS-based optimization model finalized to the localization of new power-to-gas plants: The case study of Sicily (Italy)
2022
In 2020 the European Commission approved the GND (Green New Deal), a strategic plan aimed at the decarbonization of the EU by 2050. In this scenario, the diffusion of alternative renewable energy sources (RESs) play a key role, particularly electric energy production from wind and photovoltaic power plants. Regardless, the nonprogrammable nature of these power sources led recent studies to focus the interest on the power-to-gas solution (PtG), consisting of the electrolytic transformation of excesses of unused electric energy into hydrogen. The complexity of this technology requires the development of strategic optimization models aimed at finding the best locations for new PtG plants in sp…
Determination of molecular weights and Stokes' radii of non-denatured proteins by polyacrylamide gradient gel electrophoresis 3. Estimation of the up…
1985
Studying the separation behavior of various native carbonic anhydrase isozymes from mammalian erythrocytes we found that the migration of these enzymes differs from that of the marker proteins commonly used in gradient gel electrophoresis. In alkaline buffer systems the enzymes from human, bovine, rabbit, and canine erythrocytes start to migrate with a size apparently 6 to 12 times larger than their monomeric size, then gradually lose in apparent size and finally end up in a size equivalent to their monomeric mol mass. We determined the monomeric mol mass of the various carbonic anhydrase forms to be 23 000 to 39 000 (g/mol). These values are in accordance with different data in the literat…
Efficient Expression and Crystallization System of Cancer-Associated Carbonic Anhydrase Isoform IX.
2015
Human carbonic anhydrase IX (CA IX) is overexpressed in a number of solid tumors and is considered to be a marker for cellular hypoxia that it is not produced in most normal tissues. CA IX contributes to the acidification of the extracellular matrix, which, in turn, favors tumor growth and metastasis. Therefore, CA IX is considered to be a promising anti-cancer drug target. However, the ability to specifically target CA IX is challenging due to the fact that the human genome encodes 15 different carbonic anhydrase isoforms that have a high degree of homology. Furthermore, structure-based drug design of CA IX inhibitors so far has been largely unsuccessful due to technical difficulties regar…
5-Substituted-benzylsulfanyl-thiophene-2-sulfonamides with effective carbonic anhydrase inhibitory activity: Solution and crystallographic investigat…
2017
Abstract A series of 5-substituted-benzylsulfanyl-thiophene-2-sulfonamides was prepared by reacting 5-bromo-thiophene-2-sulfonamide with 5-substituted-benzyl mercaptans. The new compounds were investigated as carbonic anhydrase (CA, EC 4.2.1.1) inhibitors. The cytosolic human (h) isoforms hCA I was poorly inhibited by the new sulfonamides (KIs in the range of 683–4250 nM), whereas hCA II, and the transmembrane, tumor associated isoforms hCA IX and XII were effectively inhibited in the subnanomolar–nanomolar range. A high resolution X-ray crystal structure of the adduct of hCA II with one of the new sulfonamides allowed us to rationalize the excellent inhibitory activity of these heterocycli…
N-Substituted and ring opened saccharin derivatives selectively inhibit transmembrane, tumor-associated carbonic anhydrases IX and XII.
2017
A series of N-substituted saccharins incorporating aryl, alkyl and alkynyl moieties, as well as some ring opened derivatives were prepared and investigated as inhibitors of the metalloenzyme carbonic anhydrase (CA, EC 4.2.1.1). The widespread cytosolic isoforms CA I and II were not inhibited by these sulfonamides whereas transmembrane, tumor-associated ones were effectively inhibited, with KIs in the range of 22.1-481nM for CA IX and of 3.9-245nM for hCA XII. Although the inhibition mechanism of these tertiary/secondary sulfonamides is unknown for the moment, the good efficacy and especially selectivity for the inhibition of the tumor-associated over the cytosolic, widespread isoforms, make…
3H-1,2-benzoxathiepine 2,2-dioxides: a new class of isoform-selective carbonic anhydrase inhibitors
2017
Abstract A new chemotype with carbonic anhydrase (CA, EC 4.2.1.1) inhibitory action has been discovered, the homo-sulfocoumarins (3H-1,2-benzoxathiepine 2,2-dioxides) which have been designed considering the (sulfo)coumarins as lead molecules. An original synthetic strategy of a panel of such derivatives led to compounds with a unique inhibitory profile and very high selectivity for the inhibition of the tumour associated (CA IX/XII) over the cytosolic (CA I/II) isoforms. Although the CA inhibition mechanism with these new compounds is unknown for the moment, we hypothesize that it may be similar to that of the sulfocoumarins, i.e. hydrolysis to the corresponding sulfonic acids which therea…
The shell-forming proteome of Lottia gigantea reveals both deep conservations and lineage-specific novelties
2013
19 pages; International audience; Proteins that are occluded within the molluscan shell, the so-called shell matrix proteins (SMPs), are an assemblage of biomolecules attractive to study for several reasons. They increase the fracture resistance of the shell by several orders of magnitude, determine the polymorph of CaCO(3) deposited, and regulate crystal nucleation, growth initiation and termination. In addition, they are thought to control the shell microstructures. Understanding how these proteins have evolved is also likely to provide deep insight into events that supported the diversification and expansion of metazoan life during the Cambrian radiation 543 million years ago. Here, we p…
The Variscan structural and metamorphic evolution of the eastern Southalpine basement
1994
The basement of the Southern Alps (northern Italy) belongs to the southernmost part of the European Variscan mountain belt. In contrast to other areas of the Alps, the post-Variscan metamorphic and tectonic overprint is weak and therefore permits the unravelling of the Variscan tectonometamorphic evolution of this region. Overprinting criteria and the mapping of the penetrative structural elements (S2 and L2) allow three deformational events to be distinguished. Major Variscan deformation (D2) in the Southern Alps commenced in the Carboniferous and lasted until the end of the Late Carboniferous. Tectonic movement during D2 was north-directed and was accompanied and followed by greenschist f…
Timing of granitoid magmatism in the eastern mid-German crystalline rise
2001
Abstract The eastern parts of the mid-German crystalline rise (MGCR) are exposed in the Spessart, Ruhla and Kyffhauser crystalline complexes and known from boreholes in the region of Dessau. In this study we determined igneous formation ages of granitoid intrusives and orthogneisses from this part of the MGCR using the Pb/Pb single zircon dating technique. The intrusion ages of the granitoids range from ∼489 to ∼302 Ma (i.e. from lower Ordovician to upper Carboniferous). The grouping of these ages marks several distinct magmatic episodes during the Variscan orogeny, with a major peak at the end of the lower Carboniferous. We tentatively suggest the following geodynamic scenario for the orig…
Geochronology of the mid-German crystalline rise west of the River Rhine
1996
The mid-German crystalline rise has its westernmost exposures at the western margin of the Rhine graben in the southern Pfalz and the northern Alsace. The outcrops are made up of granitoid rocks and minor volcano-sedimentary sequences. Radiometric ages obtained by U/Pb, Pb/Pb, Sm/Nd and Rb/Sr analyses of the igneous rocks from this area range from ∼433 to ∼325 Ma thus covering a time span from the Silurian to the end of the lower Carboniferous. Because the investigated rocks are — according to their chemical composition — largely related to subduction zone environments, the following three geodynamical scenarios are postulated, always taking subduction of oceanic crust beneath the mid-Germa…