Search results for "Catha"

La musicoterapia nella Grecia antica

2007

Music in ancient Greece was believed to heal both the soul and the body, and especially to soothe wrath and low spirits. According to the ethos theory, music has a prominent role in the education of the young people. With both its medical and magic connotations, the notion of musical catharsis is involved in the performance of music and dance in the Dionysiac rites. The medical thought on music therapy is mainly linked with theories concerning the pulse, where musicology and medicine share a common ground.

Validazione e Verifica (V&V) di CATHARE2 e TRACE sul Programma Sperimentale SPES-2

2014

Structure-based engineering of strictosidine synthase: auxiliary for alkaloid libraries.

2007

SummaryThe highly substrate-specific strictosidine synthase (EC 4.3.3.2) catalyzes the biological Pictet-Spengler condensation between tryptamine and secologanin, leading to the synthesis of about 2000 monoterpenoid indole alkaloids in higher plants. The crystal structure of Rauvolfia serpentina strictosidine synthase (STR1) in complex with strictosidine has been elucidated here, allowing the rational site-directed mutation of the active center of STR1 and resulting in modulation of its substrate acceptance. Here, we report on the rational redesign of STR1 by generation of a Val208Ala mutant, further describing the influence on substrate acceptance and the enzyme-catalyzed synthesis of 10-m…

Improved Expression of His6-Tagged Strictosidine Synthase cDNA for Chemo-Enzymatic Alkaloid Diversification

2010

Strictosidine synthase (STR1) catalyzes the stereoselective formation of 3alpha(S)-strictosidine from tryptamine and secologanin. Strictosidine is the key intermediate in the biosynthesis of 2,000 plant monoterpenoid indole alkaloids, and it is a key precursor of enzyme-mediated synthesis of alkaloids. An improved expression system is described which leads to optimized His(6)-STR1 synthesis in Escherichia coli. Optimal production of STR1 was achieved by determining the impact of co-expression of chaperones pG-Tf2 and pG-LJE8. The amount and activity of STR1 was doubled in the presence of chaperone pG-Tf2 alone. His(6)-STR1 immobilized on Ni-NTA can be used for enzymatic synthesis of stricto…

Molecular docking and pharmacogenomics of vinca alkaloids and their monomeric precursors, vindoline and catharanthine.

2011

International audience; Vinblastine and vincristine are dimeric indole alkaloids derived from (formerly: ). Their monomeric precursor molecules are vindoline and catharanthine. While vinblastine and vincristine are well-known mitotic spindle poisons, not much is known about vindoline and catharanthine. Vindoline and catharanthine showed weak cytotoxicity, while vinblastine, vincristine, and the semisynthetic vindesine and vinorelbine revealed high cytotoxicity towards cancer cells. This may reflect a general biological principle of poisonous plants. Highly toxic compounds are not only active towards predators, but also towards plant tissues. Hence, plants need mechanisms to protect themselv…

Isolation and characterization of intergeneric somatic hybrids in the Apocynaceae family

1991

Protoplasts were isolated from callus cultures of Rauwolfia serpentina Benth., Rhazya stricta Decaisne, and Catharanthus roseus (L.) G. Don, or from leaves of Vinca minor L. Protoplast isolation, culture, and fusion techniques as well as hybrid screening systems were developed for these species, and hybrids were obtained. Hybrid combinations were Rauwolfia + Vinca, Rauwolfia + Catharanthus, Rauwolfia + Rhazya, and Catharanthus + Vinca. For hybrid isolation, the physiological complementation method was utilized. Analyses of the material obtained included a cytogenetic study of the chromosomes, a study of multiple molecular forms of transferases and esterases, and the blot hybridization of re…

Le Catharisme : fondation d'un mythe politique

1998

Le temps de la commémoration ou la tentative impossible du partage de la souffrance. Exemple de la commémoration des 20 ans du génocide de Srebrenica…

2017

National audience

Strumenti Musicali/ Musical Instruments

2021

Nella Grecia antica la musica ricopriva un ruolo molto importante in ogni contesto significativo della vita comunitaria; allo stesso modo era presente in ogni evento rilevante dell’esistenza individuale. Gli strumenti musicali furono classificati da Aristosseno di Taranto (4° sec. a.C.) in tre gruppi principali: cordofoni, aerofoni e membranofoni (classificazione ancora in uso). Almeno a partire dal 5° sec. a.C., caratteristiche etiche furono associate alla lira e all’aulos, gli strumenti più rappresentativi dei primi due gruppi. A Roma la musica ebbe un ruolo più limitato. In ancient Greece, music played a very important role in every meaningful context of community life; similarly, it was…

El Gran Catharro de 1580 ¿gripe o pertussis?

2005

The gran Catharro (1580) is considered the first influenza epidemic. The analysis of various testimonies of the time may offer some doubts about whether or not it was so; and data strongly support that it was a pertussis epidemic.