Search results for "Cdc48"
showing 10 items of 10 documents
Study of the role of the CDC48 chaperone protein in plant immunity
2018
The chaperone protein CDC48 (Cell division cycle 48) is a major regulator of the quality control of proteins and is involved in various cellular processes in animals and yeast. In contrast, the role of CDC48 in plants is poorly known. In the present work, we investigated the function of CDC48 in plant immunity thanks to the cryptogein/tobacco biological model, cryptogein being produced by the oomycete phytophthora cryptogea.Three strategies were carried out. First, the dynamic of accumulation CDC48 together with intracellular events inherent to the immune response were analyzed in both wild-type and CDC48 overexpressing tobacco cells (CDC48-TAP line). Second, a list if CDC48 partners was es…
New insights about the role of the chaperon-like protein Cdc48, a target for nitric oxide in plant immunity
2015
Nitric oxide inhibits the ATPase activity of the chaperone-like AAA+ ATPase CDC48, a target for S-nitrosylation in cryptogein signalling in tobacco c…
2012
NO has important physiological functions in plants, including the adaptative response to pathogen attack. We previously demonstrated that cryptogein, an elicitor of defence reaction produced by the oomycete Phytophthora cryptogea , triggers NO synthesis in tobacco. To decipher the role of NO in tobacco cells elicited by cryptogein, in the present study we performed a proteomic approach in order to identify proteins undergoing S-nitrosylation. We provided evidence that cryptogein induced the S-nitrosylation of several proteins and identified 11 candidates, including CDC48 (cell division cycle 48), a member of the AAA+ ATPase (ATPase associated with various cellular activities) family. In vit…
GAPDH, ntosak and cdc48, a conserved chaperone-like aaa-atpase, as nitric oxide targets in response to (a)biotic stresses
2013
Emerging functions of nitric oxide in plant immunity
2016
SPEIPMUBAgrosupCNRS; The importance of nitric oxide (NO) in innate and adaptive immunity in mammals is well recognised. NO exerts antimicrobial properties against invaders but also displays immunoregulatory functions in which S-nitrosylation represents a signalling process of major importance. Over the last two decades, a growing body of evidence suggests that NO is also a major component of plant immunity. Our understanding of its role in plant defence has been enriched by the identification and functional analysis of S-nitrosylated proteins. The recent identification of new S-nitrosylated proteins including the chaperone-like enzyme cell division cycle 48 (CDC48), histone deacetylases (HD…
Study of the chaperone protein CDC48 and its involvement in plant immunity
2018
CDC48 is a conserved chaperone protein belonging to the AAA+ ATPase family (ATPase associated with various activities). This protein uses binding and hydrolysis of ATP to generate forces to affect the transformation of polypeptide substrate in numerous cellular processes. Studies on mammalian CDC48 orthologue revealed that it recognizes ubiquitylated polypeptides, directly or via partners, leading to substrate degradation or recycling. In plants, functions of CDC48 is less understood. The aim of my thesis, is to decipher the role of CDC48 in plant defense response context. First, I have to characterize NtCDC48 in tobacco (Nicotiana tabacum) suspension cells elicited by cryptogein, an elicit…
Etude du rôle de la protéine CDC48 dans l'immunité des plantes
2018
The chaperone protein CDC48 (Cell division cycle 48) is a major regulator of the quality control of proteins and is involved in various cellular processes in animals and yeast. In contrast, the role of CDC48 in plants is poorly known. In the present work, we investigated the function of CDC48 in plant immunity thanks to the cryptogein/tobacco biological model, cryptogein being produced by the oomycete phytophthora cryptogea.Three strategies were carried out. First, the dynamic of accumulation CDC48 together with intracellular events inherent to the immune response were analyzed in both wild-type and CDC48 overexpressing tobacco cells (CDC48-TAP line). Second, a list if CDC48 partners was es…
Functional characterization of the chaperon-like protein Cdc48 in cryptogein-induced immune response in tobacco
2017
SPEIPMUBINRASUPDATDOCT; Cdc48, a molecular chaperone conserved in different kingdoms, is a member of the AAA+ family contributing to numerous processes in mammals including proteins quality control and degradation, vesicular trafficking, autophagy and immunity. The functions of Cdc48 plant orthologues are less understood. We previously reported that Cdc48 is regulated by S-nitrosylation in tobacco cells undergoing an immune response triggered by cryptogein, an elicitin produced by the oomycete Phytophthora cryptogea. Here, we inv estigated the function of NtCdc48 in cryptogein signalling and induced hypersensitive-like cell death. NtCdc48 was found to accumulate in elicited cells at both th…
The chaperone-like protein Cdc48 regulates ubiquitin-proteasome system in plants
2021
The chaperone-like protein Cdc48 regulates ubiquitin-proteasome system in plants.
2021
The degradation of misfolded proteins is mainly mediated by the ubiquitin-proteasome system (UPS). UPS can be assisted by the protein Cdc48 but the relationship between UPS and Cdc48 in plants has been poorly investigated. Here, we analyzed the regulation of UPS by Cdc48 in tobacco thanks to two independent cell lines overexpressing Cdc48 constitutively and plant leaves overexpressing Cdc48 transiently. In the cell lines, the accumulation of ubiquitinated proteins was affected both quantitatively and qualitatively and the amount of proteasomal subunits was modified, while proteolytic activities were unchanged. Similarly, the over-expression of Cdc48 in planta impacted the accumulation of ub…