Search results for "Cell Nucleus"

Recruitment of glutathione into the nucleus during cell proliferation adjusts whole-cell redox homeostasis in Arabidopsis thaliana and lowers the oxi…

2010

Summary Cellular redox homeostasis and signalling are important in progression of the eukaryotic cell cycle. In animals, the low-molecular-weight thiol tripeptide glutathione (GSH) is recruited into the nucleus early in the cell proliferation cycle. To determine whether a similar process occurs in plants, we studied cell proliferation in Arabidopsis thaliana. We show that GSH co-localizes with nuclear DNA during the proliferation of A. thaliana cells in culture. Moreover, GSH localization in the nucleus was observed in dividing pericycle cells of the lateral root meristem. There was pronounced accumulation of GSH in the nucleus at points in the growth cycle at which a high percentage of the…

Zur Ultrastruktur des Neurofibroms

1970

Elektronenmikroskopische Untersuchung eines Neurofibroms von einem 72 jahrigen Mann mit Morbus Recklinghausen. Der Tumor besteht aus verzweigten Schwannschen Zellen, die in ein kollagenfasriges Zwischengewebe eingelagert sind. Die Schwannschen Zellen sind von einer Basalmembran und einen Reticulinfasernetz umgeben. Wahrscheinlich sind die Schwannschen Zellen eines Neurofibroms befahigt fibrillare Zwischensubstanz zu bilden.

26th Wilhelm Bernhard Workshop on the Cell Nucleus 20-24 May 2019

2019

Primary Neural Precursors and Intermitotic Nuclear Migration in the Ventricular Zone of Adult Canaries

1998

New neurons continue to be born in the ventricular zone (VZ) of the lateral ventricles in the brain of adult birds. On the basis of serial section reconstruction and electron microscopy, we determined that the VZ of the adult canary brain is composed of three main cell types (A, B, and E). Type A cells were never found in contact with the ventricle and had microtubule-rich processes typical of young migrating neurons. Type B cells were organized as a pseudostratified epithelium, all contacted the ventricle, and most had a characteristic single cilium. Type E cells, also in contact with ventricle, were ultrastructurally similar to the mammalian multiciliated ependymal cells. After six inject…

A nuclear glutathione cycle within the cell cycle

2010

The complex antioxidant network of plant and animal cells has the thiol tripeptide GSH at its centre to buffer ROS (reactive oxygen species) and facilitate cellular redox signalling which controls growth, development and defence. GSH is found in nearly every compartment of the cell, including the nucleus. Transport between the different intracellular compartments is pivotal to the regulation of cell proliferation. GSH co-localizes with nuclear DNA at the early stages of proliferation in plant and animal cells. Moreover, GSH recruitment and sequestration in the nucleus during the G1- and S-phases of the cell cycle has a profound impact on cellular redox homoeostasis and on gene expression. F…

The ultrastructure of the thread-hairs on the cerci of the cockroach Periplaneta americana L.: The intermoult phase

1976

The structure of the thread-hairs on the cerci of the cockroach Periplaneta american during the intermoult phase has been investigated by scanning and transmission electron microscopy. The present study demonstrates that to each thread-hair belong one sensory cell, two glial cells, and three enveloping cells. The data indicate that: (a) the inner glial cells, which may have a trophic function, sends fingerlike processes into the cytoplasm of the senory cell body; (b) the internal enveloping cell forms the dendritic sheath; (c) the middle enveloping cell corresponds to the trichogen cell and the external enveloping cell to the tormogen cell; (d) the outer dendritic segment terminates in a ca…

The muscleblind gene participates in the organization of Z-bands and epidermal attachments of Drosophila muscles and is regulated by Dmef2.

1998

We report the embryonic phenotype of muscleblind (mbl), a recently described Drosophila gene involved in terminal differentiation of adult ommatidia. mbl is a nuclear protein expressed late in the embryo in pharyngeal, visceral, and somatic muscles, the ventral nerve cord, and the larval photoreceptor system. All three mbl alleles studied exhibit a lethal phenotype and die as stage 17 embryos or first instar larvae. These larvae are partially paralyzed, show a characteristically contracted abdomen, and lack striation of muscles. Our analysis of the somatic musculature shows that the pattern of muscles is established correctly, and they form morphologically normal synapses. Ultrastructural a…

Combination of molecular data support the existence of three main lineages in the phylogeny of aphids (Hemiptera: Aphididae) and the basal position o…

2010

Abstract The first molecular studies on the phylogeny of aphids (Hemiptera: Aphididae) bumped into a striking lack of phylogenetic structure for taxa levels higher than tribe, probably as a consequence of the rapid adaptive radiation that this group of insects went through during the Late Cretaceous. Here we present a new attempt to infer the relationships between major aphid taxa by the separate and combined analysis of two nuclear sequences (the long-wavelength opsin gene and the elongation factor 1α gene) and two mitochondrial sequences (the genes encoding the subunit 6 of the F-ATPase and the subunit II of the cytochrome oxidase). Our results confirm previous results with the grouping o…

Phylogeny of long-tailed tits and allies inferred from mitochondrial and nuclear markers (Aves: Passeriformes, Aegithalidae)

2010

Abstract In this paper we provide a molecular phylogeny based on three mitochondrial and three nuclear markers for all long-tailed tit species of the genus Aegithalos including several doubtful subspecies (17 taxa) plus three close allies of SE Asian Leptopoecile and North American Psaltriparus . Genus Aegithalos is divided into three major clades, two of them showing only minor differentiation. Separation of two mitchondrial haploytpe clusters in the N Palearctic Long-tailed Tit, Ae. caudatus , was dated back to the Late Pleistocene, however, descendants from both lineages underwent a rapid post-Pleistocene range expansion and largely mixed over the entire distribution area. The Chinese po…

p53 is involved in regulation of the DNA repair gene O6-methylguanine-DNA methyltransferase (MGMT) by DNA damaging agents

1998

The DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) is inducible by genotoxic stress. MGMT induction results from transcriptional activation of the MGMT gene which is a specific response to DNA damage. A possible factor involved in triggering MGMT induction might be p53, because both p53 and MGMT are activated by DNA breaks. To study the effect of p53 on induction of the MGMT gene, we compared the presence of functional wild-type (wt) and mutant p53 with MGMT expression level in various mouse fibroblasts and rat hepatoma cell lines upon genotoxic treatment. Cells which responded to ionizing radiation (IR) by MGMT induction displayed functional p53, whereas in cells not expr…