Search results for "Cellbiologi"

showing 4 items of 4 documents

New In-Depth Analytical Approach of the Porcine Seminal Plasma Proteome Reveals Potential Fertility Biomarkers

2018

A complete characterization of the proteome of seminal plasma (SP) is an essential step to understand how SP influences sperm function and fertility after artificial insemination (AI). The purpose of this study was to identify which among characterized proteins in boar SP were differently expressed among AI boars with significantly different fertility outcomes. A total of 872 SP proteins, 390 of them belonging specifically to Sus Scrofa taxonomy, were identified (Experiment 1) by using a novel proteomic approach that combined size exclusion chromatography and solid-phase extraction as prefractionation steps prior to Nano LC-ESI-MS/MS analysis. The SP proteomes of 26 boars showing significan…

Male0301 basic medicineSpectrometry Mass Electrospray IonizationLitter SizeProteomeBOARSwinemedicine.medical_treatmentmedia_common.quotation_subjectGene ExpressionMedical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology) Molecular Biology Microbiology Biochemistry or Biopharmacy)FertilityBiochemistryAndrology03 medical and health sciencesSemenmedicineAnimalsseminal plasma; proteome; fertility; pigMedicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi) molekylärbiologi mikrobiologi biokemi eller biofarmaci)FurinInsemination Artificialmedia_commonbiologyGene Expression ProfilingArtificial inseminationSolid Phase ExtractionMs analysisMolecular Sequence AnnotationGeneral ChemistrySpermatozoaSpermSemen AnalysisFertilityGene Ontology030104 developmental biologyProteomeChromatography GelSperm Motilitybiology.proteinFemaleBiomarkersJournal of Proteome Research
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Kel1 is a phosphorylation-regulated noise suppressor of the pheromone signaling pathway.

2021

Abstract Mechanisms have evolved that allow cells to detect signals and generate an appropriate response. The accuracy of these responses relies on the ability of cells to discriminate between signal and noise. How cells filter noise in signaling pathways is not well understood. We have analyzed noise suppression in the yeast pheromone signaling pathway. By combining synthetic genetic array screening, mass spectrometry and single-cell time-resolved microscopy, we discovered that the poorly characterized protein Kel1 serves as a major noise suppressor of the pathway. At the molecular level, Kel1 suppresses spontaneous activation of the pheromone response by inhibiting membrane recruitment of…

Programmed cell deathSaccharomyces cerevisiae ProteinsChemistryCellbiologiCell BiologySaccharomyces cerevisiaeSynthetic genetic arrayGeneral Biochemistry Genetics and Molecular BiologyPheromonesCell biologylaw.inventionlawFus3SuppressorPhosphorylationPheromoneSignal transductionMitogen-Activated Protein KinasesPhosphorylationNoiseSte5Adaptor Proteins Signal TransducingCyclin-Dependent Kinase Inhibitor ProteinsSignal TransductionCell reports
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Multi-laboratory experiment PME11 for the standardization of phosphoproteome analysis

2022

6 p.-2 fig.-2 tab.

ProteomicsenrichmentStandardizationProteomeComputer scienceCellbiologiMass-spectrometryBiophysics610 Medicine & healthComputational biologyBioinformatik och systembiologiProteòmicaProteomics:Chemical Phenomena::Biochemical Phenomena::Phosphorylation [PHENOMENA AND PROCESSES]BiochemistryExperimentFosforilació:Natural Science Disciplines::Biological Science Disciplines::Biochemistry::Proteomics [DISCIPLINES AND OCCUPATIONS]:Investigative Techniques::Epidemiologic Methods::Epidemiologic Research Design::Reproducibility of Results [ANALYTICAL DIAGNOSTIC AND THERAPEUTIC TECHNIQUES AND EQUIPMENT]Inter-laboratoryPhosphorylationquality controlEpidemiologia610 Medicine & healthBioinformatics and Systems BiologyFosfoproteïnes:fenómenos químicos::fenómenos bioquímicos::fosforilación [FENÓMENOS Y PROCESOS]:disciplinas de las ciencias naturales::disciplinas de las ciencias biológicas::bioquímica::proteómica [DISCIPLINAS Y OCUPACIONES]PhosphoproteomicsBiochemistry and Molecular BiologyReproducibility of ResultsCell BiologyReference Standards:técnicas de investigación::métodos epidemiológicos::diseño de la investigación epidemiológica::reproducibilidad de los resultados [TÉCNICAS Y EQUIPOS ANALÍTICOS DIAGNÓSTICOS Y TERAPÉUTICOS]NormalitzacióPhosphoproteinsStandardizationReference samplePhosphoproteomeProteomeLaboratory experimentLaboratoriesBiokemi och molekylärbiologi
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Vesiclepedia:A Compendium for Extracellular Vesicles with Continuous Community Annotation

2012

Vesiclepedia is a community-annotated compendium of molecular data on extracellular vesicles.

human proteinpedia; exosomal proteins; delivery-system; breast-milk; url decay; cells; microvesicles; update; rna; biogenesisQH301-705.5Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology) Molecular Biology Microbiology Biochemistry or Biopharmacy)610 Medicine & healthApoptosisComputational biology1100 General Agricultural and Biological SciencesBiologyVesiclepediaExosomesExosomeGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciencesAnnotation0302 clinical medicineSDG 3 - Good Health and Well-being1300 General Biochemistry Genetics and Molecular Biology2400 General Immunology and MicrobiologyCommunity PageExoCartaBiology (General)Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi) molekylärbiologi mikrobiologi biokemi eller biofarmaci)BiologyBiología y Biomedicina030304 developmental biologyUncategorized0303 health sciencesGeneral Immunology and MicrobiologyGeneral NeuroscienceMicrovesicleResearch2800 General NeuroscienceExtracellular vesicle11359 Institute for Regenerative Medicine (IREM)Extracellular vesiclesMicrovesiclesCompendiumCell biologyData sharingDatabases as Topic030220 oncology & carcinogenesisGeneral Agricultural and Biological SciencesExtracellular Space
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