Search results for "Cellodextrin"

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Cellobiose fermentation by Saccharomyces cerevisiae: Comparative analysis of intra versus extracellular sugar hydrolysis

2018

Abstract A prevalent procedure for the production of second generation bioethanol makes use of engineered yeast strains capable to hydrolyze cellobiose either in the cytosol or extracellularly. These two approaches have been compared in this study. For intracellular cellobiose hydrolysis, we initially tested three recombinant Saccharomyces cerevisiae strains that produced different cytosolic β-glucosidases and the cellodextrin transporter Po_CdtC from Penicillium oxalicum . The strain coexpressing Po_CdtC and the β-glucosidase from Neurospora crassa (NcBgl) showed the highest cellobiase activity but its growth in cellobiose was limited by sugar intake. A search of alternative cellobiose per…

0301 basic medicinebiologyBeta-glucosidaseSaccharomyces cerevisiaeBioengineeringCellobiosebiology.organism_classificationApplied Microbiology and BiotechnologyBiochemistryYeast03 medical and health scienceschemistry.chemical_compoundHydrolysis030104 developmental biologychemistryBiochemistryCellodextrinFermentationTrichoderma reeseiProcess Biochemistry
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Elicitor and resistance-inducing activities of -1,4 cellodextrins in grapevine, comparison with -1,3 glucans and -1,4 oligogalacturonides

2007

Cellodextrins (CD), water-soluble derivatives of cellulose composed of beta-1,4 glucoside residues, have been shown to induce a variety of defence responses in grapevine (Vitis vinifera L.) cells. The larger oligomers of CD rapidly induced transient generation of H2O2 and elevation in free cytosolic calcium, followed by a differential expression of genes encoding key enzymes of the phenylpropanoid pathway and pathogenesis-related (PR) proteins as well as stimulation of chitinase and beta-1,3 glucanase activities. Most of these defence reactions were also induced by linear beta-1,3 glucans (betaGlu) and alpha-1,4 oligogalacturonides (OGA) of different degree of polymerization (DP), but the i…

0106 biological sciencesPhysiology[SDV]Life Sciences [q-bio]Plant ScienceBiology01 natural sciences03 medical and health sciencesGene expressionBotanyGRAPEVINE[SDV.BV]Life Sciences [q-bio]/Vegetal Biology030304 developmental biologychemistry.chemical_classification0303 health sciencesPhenylpropanoidINDUCED RESISTANCEOligosaccharideGlucanaseElicitor[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacyCytosolEnzymechemistryBiochemistryChitinasebiology.proteinCELLODEXTRINSDEFENCE RESPONSES010606 plant biology & botany
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