Search results for "Channels"

Mechanical and electrophysiological effects of cromakalim on the human urinary bladder.

1994

The effects of cromakalim on spontaneous and induced mechanical activity of human detrusor muscle were investigated in vitro. Cromakalim produces a concentration-related decrease of spontaneous as well as carbachol- and K(+)-evoked contractions. This is the first study to utilize the patch clamp technique to elucidate the mechanism of action of cromakalim on human detrusor cells. Cromakalim hyperpolarizes the detrusor cells by increasing the net outward current which is most likely carried by potassium ions. In the human urinary bladder, this effect is mediated by a glibenclamide-sensitive potassium channel, as glibenclamide is able to diminish the relaxant effect of cromakalim and to preve…

Extracellular site of action of phenylalkylamines on L-type calcium current in rat ventricular myocytes.

1995

The effects of the phenylalkylamines verapamil, gallopamil, and devapamil on L-type calcium currents (ICa) were studied in ventricular myocytes from rat hearts using the whole-cell patch-clamp technique. In particular, the question was addressed, whether the pharmacological binding sites for these drugs were located at the inner and/or at the outer surface of the cell membrane. Therefore, tertiary verapamil, gallopamil, and devapamil and their corresponding quaternary derivatives were applied either from the outside or the inside of the cell membrane. Extracellular application of verapamil, gallopamil and devapamil (each at 3 microM) reduced ICa to 16.1 +/- 8.6%, 11 +/- 8.9%, and 9.3 +/- 6%…

Activation of TRPC6 calcium channels by diacylglycerol (DAG)-containing arachidonic acid: A comparative study with DAG-containing docosahexaenoic acid

2006

We synthesized a diacylglycerol (DAG)-containing arachidonic acid, i.e., 1-stearoyl-2-arachidonyl-sn-glycerol (SAG), and studied its implication in the modulation of canonical transient receptor potential sub-type 6 (TRPC6) channels in stably-transfected HEK-293 cells. SAG induced the influx of Ca(2+), and also of other bivalent cations like Ba(2+) and Sr(2+), in these cells. SAG-evoked Ca(2+) influx was not due to its metabolites as inhibitors of DAG-lipase (RHC80267) and DAG-kinase (R50922) failed to inhibit the response of the same. To emphasise that SAG exerts its action via its DAG configuration, but not due to the presence of stearic acid at sn-1 position, we synthesized 1-palmitoyl-2…

Separation of presynaptic Cav2 and Cav1 channel function in synaptic vesicle exo- and endocytosis by the membrane anchored Ca2+ pump PMCA

2021

Significance Synaptic vesicle (SV) release from presynaptic terminals requires nanometer precise control of action potential (AP)–triggered calcium influx through voltage-gated calcium channels (VGCCs). SV recycling also depends on calcium signals, though in different spatiotemporal domains. Mechanisms for separate control of SV release and recycling by AP-triggered calcium influx remain elusive. Here, we demonstrate largely independent regulation of release and recycling by two different populations of VGCCs (Cav2, Cav1), identify Cav1 as one of potentially multiple calcium entry routes for endocytosis regulation, and show functional separation of simultaneous calcium signals in the nanome…

DNA-binding activities of compounds acting as enzyme inhibitors, ion channel blockers and receptor binders.

2021

The DNA-binding activities of compounds used as remedies can display DNA-protection, but also damaging effects in biological systems. The current review compiles literature data on DNA-binding activities of drugs widely used as remedies with different therapeutic indications. The compounds are classified according their mechanism of action: enzyme inhibitors, ion channel inhibitors, inhibitors of viral RNA replication and HIV protease and receptor agonists. DNA binding was reported for such widely used drugs as paracetamol, aspirin, metformin, statins and many others. The capability of the drug to bind DNA is sometimes coupled to genotoxic effects, but in some cases - to genome protection. …

Water erosion prediction by stochastic and empirical models in the Mediterranean: A case study in Northern Sicily (Italy)

2012

The present thesis aimed to explore the methodological advantages as well as limitations in applying different modelling approaches to predict water soil erosion in Mediterranean environments. The research was accomplished in the central northern part of Sicily (Italy), considering this region to be representative of Mediterranean environmental conditions. In this region soil degradation problems, due to water erosion are becoming more and more serious. Consequently, defining models being able to predict erosion susceptibility and to discriminate environmental factors causing erosion is important to protect soil resources. The prediction of the spatial distribution of soil erosion processes…

Regional effects of monetary policy in the U.S.: An empirical re-assessment

2020

Abstract This paper provides an empirical re-assessment of the regional effects of monetary policy in the U.S. We use the narrative series of Romer and Romer (2004) as a measure of monetary policy shocks and impulse response functions estimated directly from a single equation spatial model. We find that monetary policy tightening leads to a persistent decrease in regional real personal income and employment, with asymmetric effects across regions that are magnified by spatial spillovers. The magnitude of the effects depends on the period under analysis and on the direction of the monetary policy shock. We also provide evidence of the existence of the interest rate and the housing market cha…

P267Myocardial electrophysiological effects mediated by KATP channels: controversial aspects about their involvement in the protection by chronic exe…

2018

Computation of flow velocity in rough channels

2006

Accurate estimate of flow-velocity profile is of crucial importance both for scientific purposes and for solving numerous engineering problems that include, among others, sediment transport, contaminant transport, flow resistance evaluation. This paper presents a new empirical equation to represent the vertical velocity profile. The proposed equation is essentially a modified form of the well-known logarithm law of the wall and contains three parameters having a clear physical meaning. The applicability of the equation and its accuracy assessment for different hydraulic conditions, including non-uniform conditions, is verified by using experimental data obtained by different sources. The va…

Steering dynamical systems with finite plans and limited path length

2007

Complex dynamical systems can be steered by using symbolic input plans. These plans must have a finite descriptive length, and can be expressed by means of words chosen in an alphabet of symbols. In this way, such plans can be sent through a limited capacity channel to a remote system, where they are decoded in suitable control actions. The choice of this symbols is essential to efficiently encode steering plans. To this aim, in this paper, we state the problem of finding symbols maximizing the interval of points reachable by the system along paths with constrained length. We focus on the problem with two symbols, and compare the results with those produced by plans not accounting for the l…