Search results for "Chitinase"

Structural mannoproteins released by β-elimination fromCandida albicanscell walls

1994

Abstract Mild alkaline solutions (β-elimination), after removing the non-covalently bonded wall materials by hot SDS, released 13% and 26% of remaining wall proteins from mycelial and yeast cells of Candida albicans, respectively. When the β-elimination was carried out after digestion of the walls with chitinase, four-fold more proteinaceous materials were released from mycelium and a similar amount in yeast walls. The solubilized materials were shown to be highly polydisperse, and endo-glycosidase H reduced their polydispersity and molecular masses, revealing different electrophoretic patterns in yeast and mycelial cell walls. The solubilized mycelial proteins carried N-glycosidic sugar ch…

Chitin: A Structural Biopolysaccharide with Multiple Applications

2014

Chitin is a naturally occurring fibre-forming polymer that plays a protective role in many lower eukaryotes similar to that of cellulose in plants. Chemically it is a long-chain unbranched polysaccharide made of N-acetylglucosamine residues linked through β-1,4 covalent bonds; it is the second most abundant organic compound in nature, after cellulose. Taking into account the role played by chitin in different biological structures (i.e. fungal cell walls, insect peritrophic matrix, insect and crustacean cuticles, eggshells from nematodes, cyst wall of protozoa), its metabolism (biosynthesis and degradation) is essential for different morphogenetic events. Absent in vertebrates and plants, c…

Inmunidad Innata en Esclerosis Múltiple Progresiva: Papel de la Chitinase 3-like 1

2020

Introducción: La esclerosis múltiple (EM) es una enfermedad heterogénea en la que coexisten dos fenómenos: la inflamación, que se manifiesta en forma de brotes, y la progresión sostenida de la discapacidad. Es necesario identificar biomarcadores que permitan detectar la neurodegeneración subyacente a la progresión desde el inicio, pues se pone de manifiesto de forma tardía. La Chitinase 3-like 1 (CHI3L1) se ha relacionado con la progresión en la EM, pero su papel en la patogenia de la enfermedad es todavía desconocido. Objetivos: En este trabajo, estudiamos los niveles de CHI3L1 y de la Cadena ligera de Neurofilamento (NfL) en el líquido cefalorraquídeo (LCR) de pacientes con EM y analizamo…

YKL-40 as a predictor of mortality after acute coronary syndrome

2020

Hydrolytic enzyme activity of Paenibacillus sp. strain B2 and effects of the antagonistic bacterium on cell integrity of two soil-borne pathogenic fu…

2000

Paenibacillus sp. strain B2, isolated from the mycorrhizosphere of Sorghum bicolor and having an antagonistic activity towards soil-borne fungal pathogens, possessed extracellular cellulolytic, proteolytic, chitinolytic and pectinolytic enzyme activities. The eventual role of these lytic enzymes in cellular interactions between Paenibacillus sp. strain B2 and Phytophthora parasitica and Fusariumoxysporum was investigated by electron microscopy and molecular cytology. Electron microscopic observations showed that the presence of Paenibacillus sp. strain B2 resulted in disorganisation of cell walls and/or cell contents of P. parasitica and F. oxysporum. However, when P. parasitica was treated…