Search results for "Chlorophyll fluorescence"

Retrieving and Validating Leaf and Canopy Chlorophyll Content at Moderate Resolution: A Multiscale Analysis with the Sentinel-3 OLCI Sensor

2021

ESA’s Eighth Earth Explorer mission “FLuorescence EXplorer” (FLEX) will be dedicated to the global monitoring of the chlorophyll fluorescence emitted by vegetation. In order to properly interpret the measured fluorescence signal, essential vegetation variables need to be retrieved concomitantly. FLEX will fly in tandem formation with Sentinel-3 (S3), which conveys the Ocean and Land Color Instrument (OLCI) that is designed to characterize the atmosphere and the terrestrial vegetation at a spatial resolution of 300 m. In support of FLEX’s preparatory activities, this paper presents a first validation exercise of OLCI vegetation products against in situ data coming from the 2018 FLEXSense cam…

2021

Abstract Remote sensing-based measurements of solar-induced chlorophyll fluorescence (SIF) are useful for assessing plant functioning at different spatial and temporal scales. SIF is the most direct measure of photosynthesis and is therefore considered important to advance capacity for the monitoring of gross primary production (GPP) while it has also been suggested that its yield facilitates the early detection of vegetation stress. However, due to the influence of different confounding effects, the apparent SIF signal measured at canopy level differs from the fluorescence emitted at leaf level, which makes its physiological interpretation challenging. One of these effects is the scatterin…

Bidirectional sun-induced chlorophyll fluorescence emission is influenced by leaf structure and light scattering properties : a bottom-up approach

2015

Abstract Sun-induced chlorophyll fluorescence (SIF) at leaf level is emitted in both upward and downward directions in the red and far-red part of the spectrum (650–850 nm) when a leaf is illuminated from the upper leaf surface. Hence, total SIF is represented by the sum of the upward and downward emission components. Nevertheless, the downward component of leaf SIF is often not considered despite that downward fluorescence yield (↓FY) can amount up to 40% of the total fluorescence yield (FYtot). Downward SIF is mainly emitted in the far-red, since this part of fluoresced light is highly scattered within leaves, unlike red Chl fluorescence, which is mostly reabsorbed. While total FY can be …

The scattering and re-absorption of red and near-infrared chlorophyll fluorescence in the models Fluspect and SCOPE

2019

Scattering and re-absorption have been recognized as relevant aspects for the interpretation of solar induced chlorophyll fluorescence (SIF) in vegetation remote sensing. In an earlier study [Yang and Van der Tol, RSE 215, 97–108, 2018] we addressed the problem of scattering and re-absorption of near-infrared fluorescence in the vegetation canopy. In this study we analyse within-leaf re-absorption of both red and near-infrared fluorescence using the radiative transfer model Fluspect. The leaf scattering determines the ratio of backward to total leaf fluorescence emission Fb/(Fb + Ff). Fluspect reproduces this ratio with an RMSE of less than 0.1, and explains the observed dependence of the s…

Remote sensing of sunlight-induced chlorophyll fluorescence and reflectance of Scots pine in the boreal forest during spring recovery

2005

A measurement campaign to assess the feasibility of remote sensing of sunlight-induced chlorophyll fluorescence (ChlF) from a coniferous canopy was conducted in a boreal forest study site (Finland). A Passive Multi-wavelength Fluorescence Detector (PMFD) sensor, developed in the LURE laboratory, was used to obtain simultaneous measurements of ChlF in the oxygen absorption bands, at 687 and 760 nm, and a reflectance index, the PRI (Physiological Reflectance Index), for a month during spring recovery. When these data were compared with active fluorescence measurements performed on needles they revealed the same trend. During sunny days fluorescence and reflectance signals were found to be str…

The 2013 FLEX—US Airborne Campaign at the Parker Tract Loblolly Pine Plantation in North Carolina, USA

2017

The first European Space Agency (ESA) and NASA collaboration in an airborne campaign to support ESA’s FLuorescence EXplorer (FLEX) mission was conducted in North Carolina, USA during September–October 2013 (FLEX-US 2013) at the Parker Tract Loblolly Pine (LP) Plantation (Plymouth, NC, USA). This campaign combined two unique airborne instrument packages to obtain simultaneous observations of solar-induced fluorescence (SIF), LiDAR-based canopy structural information, visible through shortwave infrared (VSWIR) reflectance spectra, and surface temperature, to advance vegetation studies of carbon cycle dynamics and ecosystem health. We obtained statistically significant results for fluorescence…

Global sensitivity analysis of the SCOPE model : what drives simulated canopy - leaving sun - induced fluorescence?

2015

This study provides insight into the key variables that drive sun-induced chlorophyll fluorescence (SIF) emanating from vegetation canopies, based on a global sensitivity analysis (GSA) of the Soil-Canopy Observation of Photosynthesis and Energy (SCOPE) balance model. An updated version of the SCOPE model was used here (v1.53) which contains novel leaf physiological modules for determination of the steady state fluorescence yield: a photosynthesis model coupled with (a) submodels having empirically derived relationships, identified as TB12 for unstressed and TB12-D for drought conditions and (b) a mechanistic (MD12) submodel based on theoretical relationships. By inspecting Sobol's total or…

Red and far-red sun-induced chlorophyll fluorescence as a measure of plant photosynthesis

2015

Remote estimation of Sun-induced chlorophyll fluorescence emitted by terrestrial vegetation can provide an unparalleled opportunity to track spatiotemporal variations of photosynthetic efficiency. Here we provide the first direct experimental evidence that the two peaks of the chlorophyll fluorescence spectrum can be accurately mapped from high-resolution radiance spectra and that the signal is linked to variations in actual photosynthetic efficiency. Red and far red fluorescence measured using a novel airborne imaging spectrometer over a grass carpet treated with an herbicide known to inhibit photosynthesis was significantly higher than the corresponding signal from an equivalent untreated…

Effects of SO2 fumigations on photosynthetic CO2 gas exchange, chlorophyll a fluorescence emission and antioxidant enzymes in the lichens Evernia pru…

1999

The effects of elevated gaseous SO2 concentrations in the lichens Evernia prunastri (L.) Ach. and Ramalina farinacea (L.) Ach. were investigated by means of gas exchange, modulated chlorophyll fluorescence analysis and antioxidant enzyme assays. The response to SO2 of the studied species differed markedly. Net photosynthetic rates were more adversely affected in E. prunastri than in R. farinacea. In addition, processes dependent on thylakoid membrane integrity such as PSII-mediated electron flow and nonphotochemical quenching were reduced to a greater extent by exposure to SO2 in E. prunastri. Moreover, the ability to reoxidize the quinone pool was lower in this species. Finally, the activi…

FluorMODleaf: A new leaf fluorescence emission model based on the PROSPECT model

2010

International audience; A new model of chlorophyll a fluorescence emission by plant leaves, FluorMODleaf, is presented. It is an extension of PROSPECT, a widely used leaf optical properties model that regards the leaf as a pile of N absorbing and diffusing elementary plates. In FluorMODleaf, fluorescence emission of an infinitesimal layer of thickness dx is integrated over the entire elementary plate. The fluorescence source function is based on the excitation spectrum of diluted isolated thylakoids and on the emission spectra of isolated photosystems, PSI and PSII, which are the main pigment–protein complexes involved in the initial stages of photosynthesis. Scattering within the leaf is p…