Search results for "Chlorophyll"
showing 10 items of 453 documents
Preparation of Native and Recombinant Light-Harvesting Chlorophyll-<I>a/b </I>Complex
2004
Procedures to isolate native light-harvesting chlorophyll-a/b complex (LHCIIb) and to reconstitute recombinant LHCIIb are described. Separation of trimeric from monomeric forms and free pigment by sucrose density-gradient ultracentrifugation can be applied to both native and reconstituted complexes. The preparations are characterized by their pigment composition, protein pattern, and spectral properties.
Comparison of MODIS and Landsat-8 retrievals of Chlorophyll-a and water temperature over Lake Titicaca
2016
Chlorophyll-a concentration ([Chl-a]) and Lake Surface Temperature (LST) were retrieved in Lake Titicaca (Peru-Bolivia) using MODIS and Landsat-8 images. The lake was chosen as a case-study for evaluating the feasibility of Landsat-8 images for [Chl-a] and LST monitoring in oligotrophic and mesotrophic water bodies. The big size of the lake and its spatial and temporal variability, allowed the comparison of MODIS and Landsat-8 products for a wide range of [Chl-a] and LST. The atmospheric correction of the images was facilitated by the very high altitude of the lake. MODIS images were processed with standard ocean color algorithms whereas for Landsat-8, specific algorithms were tested and va…
The use of zooplankton metrics to determine the trophic status and ecological potential: An approach in a large Mediterranean watershed.
2021
In the European Water Framework Directive, zooplankton was not included as a Biological Quality Element despite its important place in the aquatic trophic web. In the present study on zooplankton abundances and biomasses, we used several metrics to test their ability to detect differences among trophic statuses and ecological potential levels, and collected a large sum of data in more than 60 reservoirs at Ebro watershed, on more than 300 sampling occasions over 10 years. Our results indicate that most zooplankton metrics are correlated to environmental variables that determine reservoirs’ trophic states, especially chlorophyll a and total phosphorus. The metrics with better sensitivity to …
Periphyton growth and diatom community structure in a cooling water pond
1982
Periphyton (Aufwuchs) accumulation was measured on artificial substrates in a pond in central Finland which receives warm cooling-water effluent from a power plant. The growth of periphyton was generally more rapid on the substrates during the first two weeks of colonization near the inflow of the warm water effluent than in the middle of the pond. The maximum accumulation of periphyton was in spring and autumn (dry weight maximum at warm effluent was in spring 3.5 mg DW cm−2,2.65 mg AFDW cm−2; chlorophyll a maximum 3.96 µg cm−2 was found in autumn at pond-middle station). During mid-winter months the growth was strongly limited by solar radiation, but the growth was also slow at both stati…
A multi-temporal study of chlorophyll-a concentration in the Albufera lagoon of Valencia, Spain, using Thematic Mapper data
1990
Abstract A multi-temporal study of chlorophyll-a concentration, has been made by means of Landsat-5 Thematic Mapper (TM) band 1 (0·45-0·52μm) in the Albufera of Valencia, a hypertrophic lagoon of shallow depth. The relationship between this parameter and satellite data was set up from in situ measurements and concurrent satellite data obtained in a campaign from 22 July 1985. This relation has been applied to images of different dates by using a simple procedure of correction, which is based on the use of selected surfaces whose ground reflectance is assumed to be constant with time. Good results were obtained and chlorophyll-a concentrations have been determined with an error of 20 per cen…
Phytoplankton primary production in a eutrophic cooling water pond
1984
The seasonal variation of phytoplankton photosynthesis was measured with 14C-method in a warmed ice-free pond in central Finland. Simultaneously with in situ measurements the photosynthesis was also measured in an incubator with different water temperatures and constant light (ca. 16 W m−2). The total annual photosynthesis was 57.2 C m−2 a−1. The portion of the winter and spring production of the annual photosynthesis was 18.4%, that of the autumn production ws 17.4%. Thus 64.3% of the total annual phytoplankton photosynthesis occurred in the three summer months. The range of the daily integrated photosynthesis per unit area was 1.9—563 mg C m−2d−1. The photosynthetic rate per unit chloroph…
Towards the quantitative and physically-based interpretation of solar-induced vegetation fluorescence retrieved from global imaging
2021
Due to emerging high spectral resolution, remote sensing techniques and ongoing developments to retrieve the spectrally resolved vegetation fluorescence spectrum from several scales, the light reactions of photosynthesis are receiving a boost of attention for the monitoring of the Earth's carbon balance. Sensor-retrieved vegetation fluorescence (from leaf, tower, airborne or satellite scale) originating from the excited antenna chlorophyll a molecule has become a new quantitative biophysical vegetation parameter retrievable from space using global imaging techniques. However, to retrieve the actual quantum efficiencies, and hence a true photosynthetic status of the observed vegetation, all …
Circular and linear dichroism of aggregates of chlorophyll a and chlorophyll b in 3-methylpentane and paraffin oil.
1996
A circular (CD) and linear dichroism (LD) study of the water adducts of the green plant chlorophylls a (Chl a) and b (Chl b) in hydrocarbon solvents 3-methylpentane and paraffin oil is presented. A strong red shift of the Qy-absorption band from 663 to 746 nm (1678 cm−1) is observed as the water adduct of Chl a is formed. The Chl a-water adduct shows a strong, nonconservative CD signal, which is characterized by a positive peak at 748 nm and two negative peaks at 720 and 771 nm. The maximum CD (AL - AR) is only one order of magnitude smaller than the isotropic absorption maximum. We propose that this exceptionally strong signal is the so-called psi-type CD. The LD spectrum was measured in a…
Early Steps in the Assembly of Light-harvesting Chlorophyll a/b Complex
2004
The light-harvesting chlorophyll a/b complex (LHCIIb) spontaneously assembles from its pigment and protein components in detergent solution. The formation of functional LHCIIb can be detected in time-resolved experiments by monitoring the establishment of excitation energy transfer from protein-bound chlorophyll b to chlorophyll a. To detect the possible initial steps of chlorophyll binding that may not yet give rise to chlorophyll b-to-a energy transfer, we have monitored LHCIIb assembly by measuring excitation energy transfer from a fluorescent dye, covalently bound to the protein, to the chlorophylls. In order to exclude interference of the dye with protein folding or pigment binding, th…
Energy transfer and pigment composition in three chlorophyll b-containing light-harvesting complexes isolated from Mantoniella squamata (Prasinophyce…
1986
Light-harvesting Chl a/b protein complexes were isolated from the higher plant Sinapis alba, the green alga Chlorella fusca, and the prasinophycean alga Mantoniella squamata by mild gel electrophoresis. The energy transfer from chlorophyll b and the accessory xanthophyll was measured by means of fluoresence spectroscopy at 77 K. The pigment composition of the isolated antenna complexes was determined by high performance liquid chromatography in order to calculate the number of light absorbing molecules per chlorophyll a in the different light-harvesting complexes. These results were complemented by the quantitation of the pigments in total thylakoids as well as in the different electrophore…