Search results for "Cholesterol"
showing 10 items of 1211 documents
Familial hypercholesterolæmia in children and adolescents: Gaining decades of life by optimizing detection and treatment
2015
Contains fulltext : 155263.pdf (Publisher’s version ) (Open Access) Familial hypercholesterolaemia (FH) is a common genetic cause of premature coronary heart disease (CHD). Globally, one baby is born with FH every minute. If diagnosed and treated early in childhood, individuals with FH can have normal life expectancy. This consensus paper aims to improve awareness of the need for early detection and management of FH children. Familial hypercholesterolaemia is diagnosed either on phenotypic criteria, i.e. an elevated low-density lipoprotein cholesterol (LDL-C) level plus a family history of elevated LDL-C, premature coronary artery disease and/or genetic diagnosis, or positive genetic testin…
Multicomponent Exercise Training Combined with Nutritional Counselling Improves Physical Function, Biochemical and Anthropometric Profiles in Obese C…
2020
Aerobics or strength exercise plus diet interventions have been shown to counteract childhood obesity. However, little is known with regard to periodized multicomponent exercise interventions combined with nutritional counselling, which might be less demanding but more enjoyable and respectful of children and adolescents&rsquo
Effects of Strength Training and Nutritional Counseling on Metabolic Health Indicators in Aging Women
2005
Purpose: Effects of strength training (ST) and nutritional counseling (NC) on metabolic health indicators were examined in 50 aging women. Methods: Subjects performed ST for 21 weeks. NC was given to obtain sufficient energy and protein intake, and recommended intake of fat and fiber. Results: NC increased intake of protein and polyunsaturated fat by 4.5% and 10.7% and decreased intake of saturated fat by 18.3%. Serum concentrations of total cholesterol (TC), LDL-cholesterol (LDL-C), total and HDL-cholesterol (HDL-C) ratio and triacylglycerols (TAG) decreased, and serum HDL-C increased in all subjects after ST. Respectively, systolic and diastolic blood pressure and serum insulin concentrat…
Nurse‐led counseling for coronary artery disease patients: A 1‐year follow‐up study
2021
Nurse-led counseling and systematic follow-up has been shown to reduce cardiovascular risk factor levels. The study aim was to investigate if cardiovascular risk factor levels could be reduced in patients with coronary artery disease with a nurse-led intervention and to report patients’ evaluations of nurse-led counseling. The study design was a real-life longitudinal follow-up counseling intervention. Data were collected from November 2017 to May 2020. The nurse-led intervention and patients’ follow-up time was one year. Of the 78 patients recruited, 74 completed the study. The most significant findings were in the levels of total cholesterol, low-density lipoprotein cholesterol and trigly…
CD95 death-inducing signaling complex formation and internalization occur in lipid rafts of type I and type II cells
2004
We investigated the membrane localization of CD95 in type I and type II cells, which differ in their ability to recruit and activate caspase-8. We found that CD95 was preferentially located in lipid rafts of type I cells, while it was present both in raft and non-raft plasma membrane sub-domains of type II cells. After stimulation, CD95 located in phospholipid-rich plasma membrane was recruited to lipid rafts in both types of cells. Similarly, CD95 cross-linking resulted in caspase-independent translocation of FADD/MORT1 and caspase-8 to the lipid rafts, which was prevented by a death domain-defective receptor. CD95 internalization was then rapid in type I and delayed in type II cells and s…
Improving analysis of apolar organic compounds by the use of a capillary titania-based column: Application to the direct determination of faecal ster…
2010
This article reports a new procedure for the direct determination of faecal sterols coprostanol and cholesterol in wastewater samples as tracers of human sewage contamination. The method combines in-tube solid-phase microextraction (IT-SPME) for analyte enrichment and capillary liquid chromatography (LC) for separation with diode array detection for identification and quantification. A titania-based polymeric capillary column and a conventional octadecyl silica (ODS) capillary column were evaluated and compared for their ability to separate the analytes. The titania-based column allowed the separation of the analytes in much shorter chromatographic times and with better chromatographic prof…
Microwave-assisted saponification of animal greases for cholesterol determination
1998
Abstract An alternative method for the hydrolysis of cholesterol esters in animal grease samples has been developed. The method consists of the microwave-assisted treatment of 4 g of animal fat or oil with 40 ml of an ethanolic KOH solution inside a 115 ml closed reactor in which samples were irradiated for 2.5 min at 50% power level of an exit power of 700 W. After extraction with petroleum ether and dissolution of the unsaponifiables in toluene, total cholesterol was determined spectrophotometrically by using p-anisidine as a test reagent of the enzymatic reaction of cholesterol through a bienzymic reactor in which cholesterol oxidase and horseradish peroxidase were noncovalently immobili…
Simultaneous quantification of serum phytosterols and cholesterol precursors using a simple gas chromatographic method
2012
Determination of the main phytosterols (Ps, β-sitosterol and campesterol) and cholesterol precursors (desmosterol and lathosterol) in human serum using a simple GC-FID method has been validated. Direct saponification, without lipid extraction, sterols extraction, and further derivatization was applied to samples prior to GC analysis. To evaluate the method, a pool of serum samples from eight healthy women was used. Good linearity (r>0.99) was found in the assay range: β-sitosterol (0.99–17.82 µg/mL), campesterol (0.14–10.8 µg/mL), desmosterol (0.17–2.6 µg/mL), and lathosterol (0.6–5.97 µg/mL). Limits of detection (ng/mL) were: 86 (β-sitosterol), 42 (campesterol), 4 (desmosterol), and 44 (la…
Flow injection determination of free and total cholesterol in animal greases using enzymes in non-aqueous media
1998
A non-covalently coimmobilized bienzymic reactor of horseradish peroxidase (HRP) and cholesterol oxidase (COD), operating in a continuous organic flowing stream of 1 × 10–3 M p-anisidine in buffer-saturated (pH 7.0) toluene, has been employed for cholesterol determination in animal greases, such as pig, beef, and chicken fat, and codfish liver oil. The method provides a good linear relationship up to 1.8 × 10–3 M cholesterol and average recoveries of 99.5%, a high sensitivity, with a detection limit of 1 × 10–6 M of cholesterol and a good precision (an interday RSD of 1.8% for the determination of total cholesterol in a codfish oil sample). The method permits the direct spectrophotometric d…
Personalized management of dyslipidemias in patients with diabetes-it is time for a new approach (2022)
2022
AbstractDyslipidemia in patients with type 2 diabetes (DMT2) is one of the worst controlled worldwide, with only about 1/4 of patients being on the low-density lipoprotein cholesterol (LDL-C) target. There are many reasons of this, including physicians’ inertia, including diabetologists and cardiologists, therapy nonadherence, but also underusage and underdosing of lipid lowering drugs due to unsuitable cardiovascular (CV) risk stratification. In the last several years there is a big debate on the risk stratification of DMT2 patients, with the strong indications that all patients with diabetes should be at least at high cardiovascular disease (CVD) risk. Moreover, we have finally lipid lowe…