Search results for "Citologia"

Identificazione e caratterizzazione di molecole biologicamente attive con attività antimicrobica antibiofilm e antitumorale in Posidonia oceanica e P…

2022

Differential occurrence of S100A7 in breast cancer tissues: A proteomic-based investigation

2012

Purpose The present study reports for the first time a large-scale proteomic screening of the occurrence, subcellular localization and relative quantification of the S100A7 protein among a group of 100 patients, clinically grouped for the diagnosis of infiltrating ductal carcinoma (IDC). Experimental design To this purpose, the methods of differential proteomics, Western blotting, and immunohistochemistry were used. Results The identity of two isoforms of the protein was assessed by mass spectrometry and immunologically confirmed. Moreover, we proved by immunocytochemical applications the exclusive localization of the protein within the neoplastic cells. The correlation of S100A7 expression…

Antibacterial and antitumoral activities of new organotin(IV)-Schiff bases derivatives

2018

This preliminary report shows eight complexes of triorganotin(IV): Ph3SnOH and (CH3)3SnOH with four chelating imines on new synthesis. Of these ligands, two are salen-like (four coordination sites, two imidic, two phenoxidic) [1], one is a tetradentate pyrrole derivative [2] while the fourth, a vita- min B6 derivative, is pentadentate [3]. Ligands have been characterized by means of FT-IR, UV-Vis, Fluorescence, 1H- and 13C-NMR, LC-MS ESI triple quadrupole; complexes by means FT-IR, 1H- and 119Sn-NMR, LC-MS ESI, using the isotopic distribution pattern as a discriminant [4]. Geometry and nature of coordination complexes have been also evaluated using the 119Sn chemical shifts. Solid-state syn…

Strategie di difesa attivate in risposta a stress, in embrioni di Paracentrotus lividus

2012

Sea urchin embryos are able to activate different defense strategies against stress. This model system allows to investigate numerous phenomena in multipotent cells, which interact among themselves, in their natural position, bypassing the disadvantages of isolated cells, deprived of their normal network. Cadmium (Cd) treatment triggers the accumulation of metal in embryonic cells and the activation of defense systems depending on concentration and exposure time, through the synthesis of heat shock proteins and/or the initiation of apoptosis. Here we show that Paracentrotus lividus embryos exposed to subacute/sublethal concentrations of Cd adopt autophagy as an additional stratagem to safeg…

Developmental defects induced by gadolinium ions in sea urchin embryos of phylogenetically distant species

2015

Gadolinium (Gd) is nowadays an emergent environmental pollutant: it is a metal of the lanthanide series of the elements whose chelates are commonly employed as contrast agents for magnetic resonance imaging and subsequently released into the aquatic environment. Sea urchin embryos are highly sensitive to several kinds of stressors and able to activate different defense strategies. The aim of this study was to analyze the consequences of embryo exposure to sublethal Gd concentrations. We compared the effects of Gd on the development of four phylogenetically distant sea urchin species: two Mediterranean species, Paracentrotus lividus and Arbacia lixula, and two species living in the East coas…

Toxicity induced by Gadolinium ions on sea urchin embryos: comparison among phylogenetically distant species and focus on stress response and skeleto…

2016

Pharmaceuticals are a class of emerging environmental contaminants. Gadolinium (Gd) is a lanthanide metal whose chelates are employed as contrast agents for magnetic resonance imaging, and subsequently released into the aquatic environment. We investigated the effects of exposure to sublethal Gd concentrations on the development of four phylogenetically and geographically distant sea urchin species: two Mediterranean, Paracentrotus lividus and Arbacia lixula, and two from Australia, Heliocidaris tuberculata and Centrostephanus rodgersii. Sensitivity to Gd greatly varied, with EC50 ranging from 56 nM to 132 µM across the four species. Measures of the Gd and Ca content inside embryos showed a…

Developmental abnormalities induced by Gadolinium causes a time-dependent miss-expression of regulative and structural genes in P. lividus sea urchin…

2015

Gadolinium (Gd) is a metal of the lanthanide series of the elements whose chelates are commonly used as contrast agents for magnetic resonance imaging. Its release into the aquatic milieu has posed serious concerns regarding its noxious effects, and therefore Gd is now considered an emerging environmental pollutant. The sea urchin embryo is an excellent model used in both toxicological and developmental research. We analysed the consequences of embryo exposure to sublethal concentrations of Gd on embryo development, focusing on skeletogenesis and developmental symmetry. We observed a strong inhibition of skeleton growth, frequently displayed by an asymmetrical pattern. Continuous exposure t…

Cellular and molecular bases of biomineralization in sea urchin embryos

2013

Sea urchin embryos construct their skeleton following a precise gene-regulated time- and space-dependent programme, in concert with factors promoting cell adhesion and differentiation. The biomineral is deposited in a privileged extracellular space produced by the fused filopodia processes of the primary mesenchyme cells, the only cells producing a set of necessary matrix proteins. More than ten years ago we showed for the first time that signals from ectoderm cells promoted the expression of one of the major skeleton matrix genes by the primary mesenchyme cells. Since then, many of the crucial steps of this complex activation cascade, from ectoderm cells to embryonic spicules, have been el…

Autophagy as defence strategy against cadmium stress in Paracentrotus lividus embryos.

2009

Sea urchin embryo is a developmental model that offers an excellent opportunity to investigate the possible adaptive response of cells exposed to different stress during differentiation. These embryos are able to respond to many stress by synthesizing a set of highly conserved proteins, the hsps and/or by activation of apoptosis. The exposure to cadmium trigger the accumulation of metal in embryo cells and the activation of both defence mechanisms depending on concentration and exposure time [1-4]. Recent experimental evidences demonstrate that by autophagy, a highly regulated mechanism that enhances cell survival under various environmental and cellular stress, the breakdown and recycling …

A possible role of autophagy for the execution of apoptosis in cadmium-exposed sea urchin embryos

2012

The sea urchin embryo is a suitable model system that offers an excellent opportunity to investigate different defence strategies activated in stress conditions. We previously showed that cadmium treatment provokes the accumulation of metal in dose- and time-dependent manner in embryonic cells and the activation of defence systems, such as the synthesis of HSPs and/or the initiation of apoptosis. Analysing autophagy, by neutral red, acridine orange and LC3-detection, we demonstrated that Cd-exposed embryos adopt this process as an additional stratagem to safeguard the developmental program. We observed that embryos treated with subletal Cd concentration activate a massive autophagic respons…