Search results for "Citologia"

TARGETED THERAPIES FOR INFLAMMATORY BOWEL DISEASES AND COLORECTAL CANCER: AN INCREASING NEED FOR MICROBIOTA-INTESTINAL MUTUALISM

2015

The involvement of intestinal microbiota and dysbiosis in the pathogenesis for inflammatory bowel disease (IBD) and colorectal cancer (CRC) is a well-established factto be taken into real consideration when developing targeted therapies. This review aims to depict how advances in our understanding of the role of intestinal flora in the pathogenesis of IBD and CRC are shaping up the therapeutic protocols of their management. It is demonstrated of their management. It is demostrated that there is a circadian regulation of colocyte gene expression in response to microbiota. Dysbiosis leading to a decrease in microbiome biodiversity is also described in IBD patients in IBD patients whereby thic…

A BRIEF ANATOMO-SURGICAL DISSECTION GUIDE TO HUMAN NECK: RESULTS OF THE COLLABORATION BETWEEN THE UNIVERSITY OF PALERMO AND THE UNIVERSITY OF MALTA

2018

The aim of this article is to show methods for dissection of the neck. In the summer of 2017 a group of students of the University of Palermo that have already passed the exam of Human Anatomy took a 4 weeks dissection course at the University of Malta. The students were provided with a dissection kit, video recording equipment and cameras for taking pictures. They dissected the skin, the subcutaneous tissue, the muscular bundles, the muscles, the vascular and nervous bundles, the nerves, the larynx, the trachea and the esophagus. This paper presents the results of the dissection course and a small and simple guide to young students and medical doctors who want to learn the bases of neck di…

LA MALATTIA DIVERTICOLARE

2013

La malattia diverticolare è una patologia piuttosto frequente nella popolazione occidentale. E' stimato che il 30-40% dei pazienti sottoposti ad indagini strumentali sull'apparato digerente presentino diverticoli al colon. Questi, nel 75% dei casi sono del tutto asintomatici. la diverticolosi appare strettamente connessa allo stile di vita ed alle abitudini alimentari tipiche dei paesi occidentali. a seguito di questa preferenza geografica, gli autori anglosassoni definiscono questa condizione patologica "a disease of western civilization". La diverticolosi insorge solitamente nei soggetti adulti, il trend della malattia aumenta proporzionalmente con l'età; pur tuttavia, negli ultimi anni a…

Nutrition, malnutrition and dietary interventions in inflammatory bowel disease

2014

Inflammatory Bowel Disease (IBD), which includes both Crohn's disease (CD) and Ulcerative Colitis (UC), is a chronic idiopathic inflammatory disorder affecting the gastrointestinal tract. Diet, as a source of luminal antigens, is thought to be an important factor in the pathogenesis of IBD. often the nutritional status of patients is significantly compromised, particularly in CD. several factors, including drug-nutrient interactions, disease location, symptoms, and dietary restriction can lead to protein energy malnutrition and specific nutritional deficiencies. solid evidence regarding the accountability of certain dietary components in the etiology of IBD are lacking. With regard to malnu…

Simple and fast orotracheal intubation procedure in rat

2016

Introduction: Endotracheal intubation in the rat is difficult because of extremely small size of anatomical structures (oral cavity, epiglottis and vocal cords), small inlet for an endotracheal tube and the lack of proper techniacal instruments. Matherial and Methods: In this study we used seventy rats weighthing 400-500 g. The equipment needed for intubation was an operating table, a longish of cotton, a cotton tip, orotracheal tube, neonatal laryngoscope KTR4, small animal ventilator, and isoflurane for inhalation anaesthesia. Premedication was carried out by medetomidine hydrochloride 1 mg/mL; then, thanks to a closed glass chamber, a mixture of oxygen and isoflurane was administered. By…

RECURRENT RENAL CARCINOMA MIMICKING A GOITRE: A CASE REPORT

2013

Although the thyroid is a high vascularised gland, it is not common terget of metastases from extraglandular cancer. We reported a case of a 70 year-old woman who underwnt total thyroidectomy for multinodular goitre. In the patient's clinical hystory a nephrectomy was carried out 2 years before due to unspecified causes. The histopathological examination of the thyiroid showed a pattern compatible with clear cell renal carcinoma metastasis. The patient's relatives revealed, when questioned again, that the nephrectomy was due to the presence of a clear renal cell carcinoma keep concealed to the patients. Thanks to a timely intervention, the mass was removed and a better survival was guarante…

Parkinson's Disease and Cancer

2009

Epidemiological evidence suggests a reduced incidence of many common types of cancers in individuals with Parkinson's disease (PD). Parkinson's disease and cancer are two diseases that result from an excessive signaling by one of two forces driving cells to opposite directions. PD results from the excessive death of dopaminergic neurons in the substantia nigra pars compacta (SNc) in the brain, while uncontrolled growth is the key property of cancer. Parkinson's disease is a complex disorder, probably due in most of the cases to the interaction of environment and genes. Many genes responsible for familial forms of PD are supposed to have a supportive role in regulating or maintaining the cel…

A METHOD FOR STUDYING GENETICS OF THE PREDATORY MITE PHYTOSEIULUS PERSIMILIS

2008

Two DNA extraction and purification procedures for single mite analysis, organic (phenol-chloroform) method and the Chelex® -100 DNA extraction procedure, have been compared concerning extraction efficiency, DNA purity and DNA suitability for amplification. Phenol-chloroform extraction takes a relatively long period of time (5 days) and involves the use of toxic and hazardous chemical reagents but it produces consistent results. Chelex is a chelating resin that has a high affinity for polyvalent metal ions, Singer-Sam et al. postulated that boiling a sample in the presence of chelex prevents the degradation of DNA. Both method were evaluated to extract DNA from single frozen mite or individ…

Asimmetric skeleton patterns induced by gadolinium ions in sea urchin embryos: focus on mechanisms regulating skeletogenesis and comparison among phy…

2014

Extracellular vesicles shedded by mouse mesoangioblasts have paracrine effects on different cell types

2017

Introduction Mouse mesoangioblasts are vessel associated progenitor stem cells endowed with the ability of multipotent mesoderm differentiation. We have already demonstrated that these stem cells, as all the other stem cells, are able to release in the extracellular milieu membrane vesicles (EV) containing biological active molecules, such as FGF2, MMP2/91 and Hsp702. Today takes hold the idea that the vesicles can replace stem cells opening a new scenario in regenerative medicine3. To this aim, we investigated the possible paracrine interaction of mesoangioblast EV on different cell types and their effects. Results and conclusions We have showed that mesoangioblast EV interact with human e…