Search results for "Citologia"
showing 10 items of 517 documents
p38 MAPK in cadmium-treated MDA-MB231 breast cancer cells
2008
Correlation among pAKT, pERK1/2 and DNA fragmentation index in human cumulus cells to determine oocyte competence
2018
The aim was to correlate specific biological aspects of cumulus cells isolated from individual cumulus-oocyte complexes (COCs) with the clinical outcome of the related embryos.
In Vitro Cytotoxic Effect of Aqueous Extracts from Leaves and Rhizomes of the Seagrass Posidonia oceanica (L.) Delile on HepG2 Liver Cancer Cells: Fo…
2023
Aqueous extracts from Posidonia oceanica’s green and brown (beached) leaves and rhizomes were prepared, submitted to phenolic compound and proteomic analysis, and examined for their potential cytotoxic effect on HepG2 liver cancer cells in culture. The chosen endpoints related to survival and death were cell viability and locomotory behavior, cell-cycle analysis, apoptosis and autophagy, mitochondrial membrane polarization, and cell redox state. Here, we show that 24 h exposure to both green-leaf- and rhizome-derived extracts decreased tumor cell number in a dose–response manner, with a mean half maximal inhibitory concentration (IC50) estimated at 83 and 11.5 μg of dry extract/mL, respecti…
DNA extraction from phytoseiid specimens preserved on a glass slide and molecular diagnostic
2010
This study focuses on the diagnostic of seven species of mite belonging to the family of Phytoseiidae; very important biocontrol agents of phytophagous mites. However, they are morphologically very similar and specific diagnostic is very difficult in fact using the traditional identification methods it is uncertain to identify a species especially when Authors use different characters, very often not stable in the time. In the 60’s M.me Athias-Henriot took into consideration the insemination apparatus that is considered a stable character in the time. According to her, the shape of this apparatus could be considered a major criterion for genus distinction. The aim of the present study is to…
Situazione dell’acquacoltura in Sicilia e prospettive per l’Italia e l’Europa
2018
Proteomic analysis of extracellular vesicles shed in vitro by MDA MB 231 breast carcinoma cells
2010
Cross talk between tumor cells and connective tissue plays a key role in tumor progression. The communication is due to the release of signalling molecules from both tumor cells and surrounding stromal cells. Several secreted proteins lack the N-terminal signal peptides and, therefore, they are secreted by alternative unconventional processes such as secretion mechanism mediated by vesicle shedding in the extracellular matrix. Actually, a certain number of proteins, playing roles in some aspects of tumor progression, have been found in shed vesicles. For example, EMMPRIN, carried out in vesicles shed by tumor cells, stimulates matrix metalloproteinase (MMP) production in stromal fibroblasts…
Biological and Proteomic Characterization of the Anti-Cancer Potency of Aqueous Extracts from Cell-Free Coelomic Fluid of Arbacia lixula Sea Urchin i…
2022
Echinoderms are an acknowledged source of bioactive compounds exerting various beneficial effects on human health. Here, we examined the potential in vitro anti-hepatocarcinoma effects of aqueous extracts of the cell-free coelomic fluid obtained from the sea urchin Arbacia lixula using the HepG2 cell line as a model system. This was accomplished by employing a combination of colorimetric, microscopic and flow cytometric assays to determine cell viability, cell cycle distribution, the possible onset of apoptosis, the accumulation rate of acidic vesicular organelles, mitochondrial polarization, cell redox state and cell locomotory ability. The obtained data show that exposed HepG2 cells under…
Termodinamica e Funzioni Mentali Complesse
2010
Maria Carmela Roccheri
2012
Research focus Control of autophagy during both physiological development and in stress conditions in the sea urchin embryo. Model system Sea urchin embryo and larvae in development. Education and career 1971, biology degree, University of Palermo, Italy; advisor: G. Giudice. 1972–1973, fellowship from the Italian Ministry of Public Education, Institute of Comparative Anatomy, University of Palermo. 1974–1980, associate scientist at the Institute of Comparative Anatomy, University of Palermo. 1981–2005, associate professor of cell biology, Department of Cell and Development Biology, University of Palermo. 2000–2005, member of the scientific committee for PhD in “Biotechnology applied to pre…
Vanadium Toxicity Monitored by Fertilization Outcomes and Metal Related Proteolytic Activities in Paracentrotus lividus Embryos
2022
Metal pharmaceutical residues often represent emerging toxic pollutants of the aquatic environment, as wastewater treatment plants do not sufficiently remove these compounds. Recently, vanadium (V) derivatives have been considered as potential therapeutic factors in several diseases, however, only limited information is available about their impact on aquatic environments. This study used sea urchin embryos (Paracentrotus lividus) to test V toxicity, as it is known they are sensitive to V doses from environmentally relevant to very cytotoxic levels (50 nM; 100 nM; 500 nM; 1 µM; 50 µM; 100 µM; 500 µM; and 1 mM). We used two approaches: The fertilization test (FT) and …